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Wheat Streak Mosaic Virus Cylindrical Inclusion Body Protein, M. K. Brakke, E. M. Ball, Y. H. Hsu, W. G. Langenberg
Wheat Streak Mosaic Virus Cylindrical Inclusion Body Protein, M. K. Brakke, E. M. Ball, Y. H. Hsu, W. G. Langenberg
Department of Plant Pathology: Faculty Publications
A protein of apparent molecular weight 66000 was purified from wheat plants infected with wheat streak mosaic virus. Antiserum to this protein, labelled with gold, specifically stained cylindrical inclusions in ultrathin sections of virus-infected cells. Antiserum to the Mr 66000 protein did not react with capsid protein in Western blots, nor did antiserum to capsid protein react with the Mr 66000 protein. Both antiserum reacted with homologous antigens. The concentration of the Mr 66000 protein in extracts from infected leaves was about 100 µg per g of leaves, which is higher than the usual concentration of virions.
Concentration Of Maize Chlorotic Mottle Virus Increased In Mixed Infections With Maize Dwarf Mosaic Virus, Strain B, Karen-Beth Goldberg, Myron K. Brakke
Concentration Of Maize Chlorotic Mottle Virus Increased In Mixed Infections With Maize Dwarf Mosaic Virus, Strain B, Karen-Beth Goldberg, Myron K. Brakke
Department of Plant Pathology: Faculty Publications
The concentration of maize chlorotic mottle virus (MCMV) was up to 5.4 times higher in plants infected with both MCMV and maize dwarf mosaic virus. strain R (MDMV-BE. rhan in plants infected with MCMY only. The concentration of MDMV-B was the same in doubly and singly infected plants. Plants infected with both viruses had a reduced level of chlorophyIl and a lower than normal ratio of chloroplast to cytoplasmic rRNA. Purified MCMV had an extinction coefficient of 6.7 cm2 mg-1 at 260 nm, an absorption maximum at 258 nm, minimum at 240 nm, and 25% RNA.