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Effects Of Drought On The Survival Of Rhizobium Leguminosarum Biovar Trifolii And The Nodulation Of Subterranean Clover In An Acid Soil, Carmen Bueno
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Twenty-nine Rhizobium leguminosarum biovar trifolii strains were tested for acidity tolerance in acidified liquid medium. Only 41% of the strains grew at pH 4.1. One acid-tolerant strain, USDA 2160, and one acid-sensitive strain, 162-X-103 from Nitragin Co., were inoculated on seeds of 'Nungarin', 'Seaton Park' and 'Clare' subclover cultivars. The inoculated and pelleted seeds were sown in potted Cluff soil with pH 5.7. Three desiccation levels were imposed by delaying watering for 0, 15 or 30 days. Four gravimetric soil water contents (6.0, 6.6, 10.5 and 12.5%) were maintained under a greenhouse line-source sprinkler system for 7 weeks. The desiccation …