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Initiation Of Terpenoid Synthesis In Osmophores Of Stanhopea Anfracta (Orchidaceae): A Cytochemical Study, Kenneth J. Curry

Faculty Publications

The terpenoid component of the osmophore fragrance in Stanhopea species is composed of isoprene units synthesized via the mevalonic acid pathway. Localization of hydroxymethylglutaryl CoA synthase in the pathway was achieved using a technique which elicits the formation of an electron- dense precipitate of uranyl ferrocyanide at the point where an acetyl group from acetyl CoA is transferred to acetoacetyl CoA, releasing free CoA-SH. Applying this technique to cells of the osmophore of S. anfracta resulted in a precipitate between the inner and outer mitochondrial membranes, in the smooth endoplasmic reticulum, and, to a lesser extent, on the outer surface …