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Reduction Of Lignin In Tobacco Through The Expression Of An Antisense Caffeic Acid O-Methyltransferase, Ti̇jen Talas Oğraş, Kemal Kazan, Nermi̇n Gözükirmizi
Reduction Of Lignin In Tobacco Through The Expression Of An Antisense Caffeic Acid O-Methyltransferase, Ti̇jen Talas Oğraş, Kemal Kazan, Nermi̇n Gözükirmizi
Turkish Journal of Botany
Caffeic acid O-methyltransferase (COMT) is a methylation enzyme involved in the early stages of lignin biosynthesis. To examine the effect of antisense expression of a heterologous COMT gene on lignification, an antisense construct of a cDNA encoding a caffeic acid O-methyltransferase ( pOMT8) from Stylosanthes humilis Humb., Bonpl. & Kunth Fabaceae was transferred into tobacco via Agrobacterium tumefaciens. A large number of kanamycin-resistant shoots were recovered after transformation, and randomly selected putative transgenics were analysed for stable integration of the transgene into their genomes. The analyses demonstrated that antisense expression of pOMT8 resulted in reductions in total COMT activity, which …