Life Sciences Commons^™

Protein Prenylation In The Moss Physcomitrium Patens, Zayna H. Qaissi, Anam F. Ahmed, Katherine V. Brown, Mark P. Running

Undergraduate Arts and Research Showcase

Protein prenylation is the addition of a 15 or 20 carbon lipid to a cysteine near carboxyl terminus of target proteins. Prenylation increases hydrophobicity, which facilitates membrane associations and protein-protein interaction. Protein prenylation is generally conserved among eukaryotes, and mutations in genes that carry out prenylation are lethal in animals and yeast. In plants prenylation mutations are not always lethal, but they do affect development, disease resistance, biofuel production, and drought response, among other processes of agricultural interest. To understand the evolutionary and developmental implications of plant protein prenylation, we used the sequenced, annotated, and translated genome of the moss …

Identification Of The Biological Function Of Rab-Ggt Β-Subunits By Reverse Techniques, Briana L. Seibert, Hyun Jin Jung, Mark P. Running

Undergraduate Arts and Research Showcase

Protein prenylation is a post-translational process where lipids are added to carboxyl end groups, which allows proteins to function properly in the eukaryotic cell. The job of prenylation is to help in targeting certain proteins to specific membrane along with promoting protein-protein interactions. We use reverse genetics techniques to understand the function of prenylation in plant development by examining the phenotypic changes caused by specific gene disruption. One of the excellent model organisms Physcomitrella patens (moss) is used due to its simple structure, limited tissue and cells, sequenced genome, and its high gene targeting efficiency.

Rab geranylgeranyl transferase-II (Rab-GGT) is …

The Study Of Ppal And Its Role In The Development Of Physcomitrella Patens, Susana Perez-Martinez, Christine Chen, Mark P. Running

Undergraduate Arts and Research Showcase

Protein Prenylation is the addition of lipids to select proteins that play a key role during the development of plants. There are three enzymes that play a role in protein prenylation: protein farnesyltransferase (PFT), protein geranylgeranyl-transferase-I (PGGT), and Rab geranylgeranyltransferase (Rab-GGT). However, there is a protein called PPAL that has a similar alpha subunit of PFT, PGGT, and RAB-GGT but its biochemical function is unknown. Physconmitrella patens, a type of moss, was chosen to explore the role of PpPPAL in the development process. There are two copies of PPAL found in moss. PPAL1 and PPAL2. To study the role …