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Plant Sciences

Turkish Journal of Botany

2018

Flow cytometry

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Full-Text Articles in Life Sciences

Primula Veris Plants Derived From In Vitro Cultures And From Seeds: Genetic Stability, Morphology, And Seed Characteristics, Iwona Jedrzejczyk, Maria Morozowska, Renata Nowinska, Andrzej Jagodzinski Jan 2018

Primula Veris Plants Derived From In Vitro Cultures And From Seeds: Genetic Stability, Morphology, And Seed Characteristics, Iwona Jedrzejczyk, Maria Morozowska, Renata Nowinska, Andrzej Jagodzinski

Turkish Journal of Botany

Flow cytometry measurement confirmed the genetic stability of Primula veris L. (cowslip) plants obtained during the micropropagation process. The mean 2C DNA content was 0.97 pg/2C and P. veris can be classified in the group of plants of very small genomes. Comparative morphological analysis of cowslip plants regenerated in vitro and derived from seeds was carried out in three subsequent years under common garden conditions, which limited the effects of environmental factors. The origin of specimens significantly influenced most of the morphological traits examined. Plants that originated from seeds were characterized by more intensive development rate and higher total seed …


Comparison Of The Genome Size, Endoreduplication, And Issr Marker Polymorphism In Eight Lotus (Fabaceae) Species, Erik Ducar, Monika Rewers, Iwona Jedrzejczyk, Pavol Martonfi, Elwira Sliwinska Jan 2018

Comparison Of The Genome Size, Endoreduplication, And Issr Marker Polymorphism In Eight Lotus (Fabaceae) Species, Erik Ducar, Monika Rewers, Iwona Jedrzejczyk, Pavol Martonfi, Elwira Sliwinska

Turkish Journal of Botany

Several species within the genus Lotus are important forage crops, and many are endangered or rare. Despite the high genetic diversity of the genus, identification of Lotus species is problematic because of the limited number of reliable morphological markers. In search of a quick, inexpensive, and steady method for species identification, genome size and cell cycle/endoreduplication intensity of 14 accessions belonging to eight Lotus species were estimated by flow cytometry. ISSR-PCR was also applied to find sensitive molecular markers for genetic diversity estimation. Genome size estimation revealed that Lotus species possess very small genomes and this characteristic enables the identification …