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Full-Text Articles in Life Sciences
Transferability Of Barley Retrotransposon Primers To Analyze Genetic Structure In Iranian Hypericum Perforatum L. Populations, Razea Asadkhani Mamaghani, Seyed Abolghasem Mohammadi, Saeid Aharizad
Transferability Of Barley Retrotransposon Primers To Analyze Genetic Structure In Iranian Hypericum Perforatum L. Populations, Razea Asadkhani Mamaghani, Seyed Abolghasem Mohammadi, Saeid Aharizad
Turkish Journal of Botany
Transferability of barley retrotransposon primers was investigated to analyze population structure in St. John's wort (Hypericum perforatum L.) based on inter-retrotransposon amplified polymorphism (IRAP). Seven long terminal repeat (LTR) retrotransposon primers derived from the barley genome were used to detect genetic polymorphism in eight Iranian populations and three cultivars (Helos, New Stem, and Topaz) of H. Perforatum based on IRAP analysis. Nine possible LTR primers/primer combinations successfully amplified fragments from the H. Perforatum genome. In total, 311 bands of 100-3000 base pairs were amplified, of which 244 were polymorphic. The number of polymorphic fragments ranged from 10 (Nikita/5'LTR-2) to 57 …
Estimating Genetic Diversity Among Selected Cotton Genotypes And The Identificationof Dna Markers Associated With Resistance To Cotton Leaf Curl Disease, Ammad Abbas, Muhammad Atif Iqbal, Mehboob-Ur Rahman, Andrew H. Paterson
Estimating Genetic Diversity Among Selected Cotton Genotypes And The Identificationof Dna Markers Associated With Resistance To Cotton Leaf Curl Disease, Ammad Abbas, Muhammad Atif Iqbal, Mehboob-Ur Rahman, Andrew H. Paterson
Turkish Journal of Botany
To the extent of our knowledge, applications of DNA markers in marker-assisted breeding of cotton are handicapped due to low genetic diversity in cotton germplasm. Cotton leaf curl disease, a disease of viral origin, has substantially depressed cotton production in Pakistan, and this disease is also an emerging threat to the neighboring cotton-growing countries like China and India. The present study was designed to identify DNA markers, predominately simple sequence repeats (SSRs), associated with tolerance and/or resistance to the disease. Based upon 2 years of disease-screening field experiments, a total of 10 cotton genotypes (five highly tolerant, four highly susceptible, …