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Comparative Studies On The Competence Of Axillary Shoot Regeneration On Unsliced And Longitudinally Sliced Cotyledon Nodes Of Vigna Unguiculata, Muhammad Aasim, Sancar Fati̇h Özcan, Khalid Mahmood Khawar, Sebahatti̇n Özcan
Comparative Studies On The Competence Of Axillary Shoot Regeneration On Unsliced And Longitudinally Sliced Cotyledon Nodes Of Vigna Unguiculata, Muhammad Aasim, Sancar Fati̇h Özcan, Khalid Mahmood Khawar, Sebahatti̇n Özcan
Turkish Journal of Botany
Vigna unguiculata is an important food legume crop in the semiarid tropics. It suffers from a host of agricultural constraints including damage due to diseases and pests. Judicious application of biotechnological methods can lead to considerable improvement in this important crop. Shoot regeneration from unsliced and longitudinally sliced cotyledon node explants obtained from 3-day-old to 5-day-old seedlings grown in vitro was achieved on Murashige and Skoog (MS) medium containing 1.11, 2.22, 3.33, and 4.44 \muM benzylaminopurine (BA) supplemented with 1.0 mg/L polyvinylpyrrolidone and 500 mg/L bacteriostatic Augmentin. Callus induction was recorded in all culture media on both explants. The shoot …
In Vitro Plant Regeneration Via Petiole Callus Of Viola Patrinii And Genetic Fidelity Assessment Using Rapd Markers, Gururaj Chalageri, Uddagiri Venkanna Babu
In Vitro Plant Regeneration Via Petiole Callus Of Viola Patrinii And Genetic Fidelity Assessment Using Rapd Markers, Gururaj Chalageri, Uddagiri Venkanna Babu
Turkish Journal of Botany
Viola patrinii DC. (Violaceae) petiole explants were used for inducing calli. Significant callus proliferation was observed on MS medium supplemented with 16.12 \muM NAA and 13.33 \muM BA. Shoot regeneration was achieved upon transferring the green friable calli to MS medium with a 2-fold dilution of potassium dihydrogen phosphate supplemented with 23.25 \muM Kn and 2.68 \muM NAA. Maximum shoot regeneration was achieved in 4 weeks. Multiple shoots were separated and further cultured in half-strength MS medium supplemented with 9.85 \muM IBA with 2% sucrose. Profuse root development was observed after 20-25 days of culturing. Regenerated plants were successfully transferred …