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Articles 1 - 30 of 48
Full-Text Articles in Life Sciences
Development Of An In Vitro Regeneration System In Sorghum [Sorghum Bicolor (L.) Moench] Using Root Transverse Thin Cell Layers (Ttcls), P. Baskaran, B. Raja Rajeswari, N. Jayabalan
Development Of An In Vitro Regeneration System In Sorghum [Sorghum Bicolor (L.) Moench] Using Root Transverse Thin Cell Layers (Ttcls), P. Baskaran, B. Raja Rajeswari, N. Jayabalan
Turkish Journal of Botany
An efficient and reproducible plant regeneration system was developed from cells or tissues of agronomically important Indian sorghum genotypes including 2 commercial cultivars (NSH27 & K8) of Sorghum bicolor (L.) Moench. Callus induction and plant regeneration were achieved on transverse thin cell layers (tTCL) of roots from aseptically germinated 7-day-old seedlings. Callus response depended on the genotype, the concentrations and composition of growth substances and number of in vitro regeneration cycles undergone by the donor plant. Murashige and Skoog (MS) medium supplemented with 4.5-18.1 µM 2,4-dichlorophenoxy acetic acid (2,4-D), 5.4-21.5 µM naphthalene acetic acid (NAA), 5.7-22.8 µM indole acetic acid …
Morphological, Anatomical And Palynological Investigation On Sonchus Erzincanicus Matthews (Asteraceae), Ali̇ Kandemi̇r, Serdal Makbul, Zafer Türkmen, Murat Yilmaz
Morphological, Anatomical And Palynological Investigation On Sonchus Erzincanicus Matthews (Asteraceae), Ali̇ Kandemi̇r, Serdal Makbul, Zafer Türkmen, Murat Yilmaz
Turkish Journal of Botany
In this study, Sonchus erzincanicus Matthews (Asteraceae) collected from Erzincan (Turkey) has been investigated by its leaf and stem anatomical features, pollen grain characteristics and ecological features. It is an endemic perennial herbs growing in slightly basic with low organic content soil. Stem cortex seen transparently is composed of parenchyma cells. The leaves are bifacial and have stomata cells that are anomocytic. The pollen type of the species is trizonocolporate. All anatomical studies including transverse sections of the stems and leaves and surface section of leaves are illustrated for the firs time.
Prehistoric And Medieval Plant Remains From Two Sites On The Euphrates, South-Eastern Turkey, Emel Oybak Dönmez
Prehistoric And Medieval Plant Remains From Two Sites On The Euphrates, South-Eastern Turkey, Emel Oybak Dönmez
Turkish Journal of Botany
The results of the archaeobotanical examination of 2 sites, namely Mezraa Höyük and Gre Virike, on the Euphrates in the Karkamış (Carchemish) area, near Birecik (Şanlıurfa) in south-east Turkey are given. The cultural levels that yielded carbonised plant remains date from the Early Bronze Age (3000-2000 BC), Middle Bronze Age (2000-1500 BC) and Medieval period (11^{th} - 13^{th} centuries AD). The plant assortment in the Bronze Age did not differ essentially from that during the Medieval period. The major crop plant of the farmers was Hordeum L. (barley, hulled). Other cereal crops included Triticum dicoccum Schübl. (emmer wheat), T. monococcum …
Contribution To The Lichen Flora Of Gürgen Dağı (Çanakkale), Gülşah Çobanoğlu, Orhan Sevgi̇
Contribution To The Lichen Flora Of Gürgen Dağı (Çanakkale), Gülşah Çobanoğlu, Orhan Sevgi̇
Turkish Journal of Botany
A list of 75 lichen taxa is reported from Gürgendağı Mountain located to the north of Kazdağı (Ida Mountain), in Çanakkale province. Among the identified taxa, 41 taxa are epiphytic, 26 saxicolous and 8 terricolous. All of the taxa are new records for the area. Nineteen taxa are new for the province.
New Records Of Tricholomataceae And Cortinarius (Pers.) Gray From Turkey, Ertuğrul Sesli̇
New Records Of Tricholomataceae And Cortinarius (Pers.) Gray From Turkey, Ertuğrul Sesli̇
Turkish Journal of Botany
Lyophyllum multiforme (Peck) H.E.Bigelow, Cortinarius caesiocanescens M.M.Moser and C. corrosus Fr. were collected for the first time from Turkey.
Saponaria Bargyliana Gombault (Caryophyllaceae): A New Record For Turkey And Analysis Of Its Morphological Characters With Related Species, Bi̇rol Mutlu
Turkish Journal of Botany
Previously, Saponaria bargyliana Gombault was known only from its type locality, the north of Syria in the Nosaïris mountains, which was published by Gombault in 1962. During a field trip in June 2002 to Erzin (Hatay) district, the species was collected for the second time from a new locality far from its locus classicus. Thus, this species was described as a new record for the flora of Turkey. The description of this species was expanded and its geographical distribution, habitat, flowering time and conservation status are discussed. Quantitative and qualitative analysis of Saponaria bargyliana and closely related species is discussed. …
On The Taxonomy Of Plantaginaceae Juss. Sensu Lato: Evidence From Sem Of The Seed Coat, Azza Ahmed Shehata, Mohamed Hesham A. Loutfy
On The Taxonomy Of Plantaginaceae Juss. Sensu Lato: Evidence From Sem Of The Seed Coat, Azza Ahmed Shehata, Mohamed Hesham A. Loutfy
Turkish Journal of Botany
SEM of the seed coat was studied in 41 taxa of the Plantaginaceae sensu lato. The study included 31 taxa of Plantago L. and 10 taxa that were formerly assigned to the Scrophulariaceae, representing the genera Antirrhinum L., Digitalis L., Linaria Mill. and Veronica L. The obtained data were analysed by the NT sys-PC program package using the UPGMA clustering method. The study showed that Veronica possessed certain affinities to Plantago species, thus giving extra support to earlier views that Plantaginaceae sensu stricto and Scrophulariaceae sensu lato are allied through Veronica. The study also favoured the paraphyly of Plantago subgenus …
Macrofungi From Andırın (Kahramanmaraş) District, Abdullah Kaya
Macrofungi From Andırın (Kahramanmaraş) District, Abdullah Kaya
Turkish Journal of Botany
Macrofungi specimens were collected from Andırın (Kahramanmaraş) district in 1998-2003. As a result of field and laboratory studies, 131 taxa belonging to 35 families in Ascomycetes and Basidiomycetes were identified. Six of them, Hemimycena pithya (Fr.) Dörfelt, Macrocystidia cucumis (Pers.) Joss., Mycena maculata P.Karst., Tricholoma magnivelare (Peck) Redhead (Tricholomataceae), Entoloma lepidissimum (Svrcek) Noordel. (Entolomataceae) and Pluteus pouzarianus Singer var. albus Bonnard (Pluteaceae), are new records for Turkey.
Colonial And Morphological Characteristics Of Some Microfungal Species Isolated From Agricultural Soils In Eskişehir Province (Turkey), Semra İlhan, Rasi̇me Demi̇rel, Ahmet Asan, Cengi̇z Bayçu, Engi̇n Kinaci
Colonial And Morphological Characteristics Of Some Microfungal Species Isolated From Agricultural Soils In Eskişehir Province (Turkey), Semra İlhan, Rasi̇me Demi̇rel, Ahmet Asan, Cengi̇z Bayçu, Engi̇n Kinaci
Turkish Journal of Botany
Aspergillus crustosus Raper & Fennell, Eupenicillium egyptiacum (J.F.H.Beyma) Stolk & D.B.Scott, Paecilomyces ramosus Samson & H.C.Evans, and Penicillium novae-zeelandiae J.F.H.Beyma were examined for their colonial and morphological properties via visual, light and scanning electron microscopy. These species isolated from soil in different regions of Eskişehir are recorded for the first time in Turkey.
Morphological And Palynological Investigation On Silene Gigantea L. Var. Gigantea And Silene Behen L. (Caryophyllaceae) Distributed In Western Anatolia And Northern Cyprus, Kemal Yildiz
Turkish Journal of Botany
A comparative investigation was carried out on the morphology and palynology of Silene gigantea L. var. gigantea and Silene behen L. species of the family Caryophyllaceae distributed in Western Anatolia and Northern Cyprus. An examination conducted on S. gigantea var. gigantea revealed that the calyx length in Western Anatolian specimens was shorter than that of those from Northern Cyprus, that the basal petal blades of the Western Anatolian specimens did not have any ligula while those from Northern Cyprus did, and that the petal blades belonging to the specimens from Northern Cyprus demonstrated less clefting as compared to those from …
Floristic Diversity And Phytogeography Of The Gebel Elba National Park, South-East Egypt, Monier M. Abd El-Ghani, Kadry N. Abdel-Khalik
Floristic Diversity And Phytogeography Of The Gebel Elba National Park, South-East Egypt, Monier M. Abd El-Ghani, Kadry N. Abdel-Khalik
Turkish Journal of Botany
The floristic composition and phytogeographical analysis of the Gebel Elba National Park in the south-east corner of Egypt were studied using multivariate analysis techniques. Its flora was poorly documented; therefore, 5 recent expeditions between 1998 and 2004 were carried out, which resulted in the collection of 179 species that belong to 51 families. Six major wadis (sites) were investigated to cover adequately the territory of the Park (35,600 km2) and to attain as complete an inventory of its vascular flora as possible. The floristic composition and species diversity among the wadis showed variations in species richness, yet W. Yahameib was …
Flora Of Bozburun Mountain And Its Environs (Antalya-Isparta-Burdur, Turkey), Hüseyi̇n Faki̇r
Flora Of Bozburun Mountain And Its Environs (Antalya-Isparta-Burdur, Turkey), Hüseyi̇n Faki̇r
Turkish Journal of Botany
This paper describes a floristic study of Bozburun Mountain and its environs. The study was conducted between 1998 and 2002, during which time 2419 vascular plant samples were collected. After the identification of the specimens, the total flora was determined to consist of 645 taxa belonging to 341 genera and 86 families. The endemism rate of the 645 taxa was 16.1% (n = 104 taxa). The phytogeographical spectrum of the species was as follows: Mediterranean 207 (32.1%), Irano-Turanian 51 (7.9%) and Euro-Siberian elements 33 (5.1%). Of the species we sampled, 311 (48.2%) were widespread and the phytogeographical regions of 10 …
Macrofungi And Their Distribution In Karaman Province, Turkey, Hasan Hüseyi̇n Doğan, Celaleddi̇n Öztürk
Macrofungi And Their Distribution In Karaman Province, Turkey, Hasan Hüseyi̇n Doğan, Celaleddi̇n Öztürk
Turkish Journal of Botany
Herein, macrofungi specimens that were collected from different locations in Karaman province, Turkey between 1999 and 2001 are reported. As a result of field and laboratory studies, 202 taxa belonging to 2 classes and 41 families were identified. Of them, 11 taxa belonged to Ascomycetes and 191 to Basidiomycetes. The most common families were Tricholomataceae, with 36 species; Bolbitiaceae, with 16 species; Agaricaceae and Cortinariaceae, with 15 species each; Coprinaceae, with 13 species; and Hymenochaetaceae, with 12 species.
New Records Of The Dictyocha Antarctica Lohmann, Dictyocha Crux Ehrenberg And Nitzschia Rectilonga Takano Species From The Sea Of Marmara, Ni̇yazi̇ Deni̇z, Seyfetti̇n Taş, Tufan Koray
New Records Of The Dictyocha Antarctica Lohmann, Dictyocha Crux Ehrenberg And Nitzschia Rectilonga Takano Species From The Sea Of Marmara, Ni̇yazi̇ Deni̇z, Seyfetti̇n Taş, Tufan Koray
Turkish Journal of Botany
The silicoflagellate species Dictyocha antarctica Lohmann, Dictyocha crux Ehrenb. and the diatom species Nitzschia rectilonga Takano are reported for the first time from the coastal waters of the north-eastern Sea of Marmara (lat. 40º 45.80' N - 40º 58.00' N, long. 28º 36.00' E - 29º 13.60' E) and also from the coastal waters of Turkey. Descriptions of these species are given in this paper.
Isolation Of Trichomes From Wheat And Other Species Of Flowering Plants, Zaure G. Aytasheva, Elizaveta D. Bogdanova, Aitkali M. Kalimagambetov, Sergey V. Chekalin, Fatima A. Polimbetova
Isolation Of Trichomes From Wheat And Other Species Of Flowering Plants, Zaure G. Aytasheva, Elizaveta D. Bogdanova, Aitkali M. Kalimagambetov, Sergey V. Chekalin, Fatima A. Polimbetova
Turkish Journal of Botany
Plant hairiness or pubescence as a specific phenotypical feature related to dehydration tolerance and resistance to leaf vermin is considered in this paper. A method for the isolation of trichomes, earlier developed for Arabidopsis Heynh., was found to be appropriate for the separation of similar polarised cells from pubescent wheat, Triticum aestivum L., lines, as well as some other higher plant species. This procedure thus paves the way for the study of the molecular organisation of trichomes in wheat and other mono- and dicotyledoneous plants.
Folk Medicines In Merzifon (Amasya, Turkey), Nurten Ezer, Öykü Mumcu Arisan
Folk Medicines In Merzifon (Amasya, Turkey), Nurten Ezer, Öykü Mumcu Arisan
Turkish Journal of Botany
Natural sources used as folk medicines in Merzifon were investigated in this study. It was determined that 35 plant and 4 animal species together with 3 animal and 1 inorganic sources are being used as various preparations of folk medicines in Merzifon area. All the collected medicinal plants were identified and voucher specimens were deposited in the Herbarium of Hacettepe University, Faculty of Pharmacy (HUEF). Local names, part(s) used, methods of preparation and traditional usages of folk medicines are given in this paper. Traditional herbal medicines were mostly used for gastrointestinal system diseases, heart and circulatory system disorders. When our …
Lichens Of Gemlik, İznik, Mudanya And Orhangazi Districts In Bursa Province (Turkey), Seyhan Oran, Şule Öztürk
Lichens Of Gemlik, İznik, Mudanya And Orhangazi Districts In Bursa Province (Turkey), Seyhan Oran, Şule Öztürk
Turkish Journal of Botany
: One hundred and eighty-one lichen taxa are reported from 88 localities in Gemlik, İznik, Mudanya and Orhangazi districts, north of Bursa, Turkey. Three species are new records for Turkey: Arthonia dispersa (Schrad.) Nyl., Lecanora sambuci (Pers.) Nyl. and Opegrapha herbarum Mont.. Sixty-two species are new records for Bursa province.
Microfungi Identified From The Flora Of Ordu Province In Turkey, Şanli Kabaktepe, Zeli̇ha Bahçeci̇oğlu
Microfungi Identified From The Flora Of Ordu Province In Turkey, Şanli Kabaktepe, Zeli̇ha Bahçeci̇oğlu
Turkish Journal of Botany
In this study, 101 species of microfungi and their hosts, which were found in Ordu Province are described. The research was carried out between 2002 and 2004. Approximately 1465 vascular plant specimens, plants infected with microfungi or not, were collected from the area. At the end of the study of the host plants, 35 families, 114 genera, and 138 host plant species (151 taxa) were established. The 101 species of microfungi were observed on the collected host specimens. These species belong to 23 genera and 13 families; 24 species belong to Ascomycota, 73 to Basidiomycota, and 4 to Mitosporic Fungi …
Macrofungi Of Bekilli (Denizli) District, Saci̇t Köse, Kudret Gezer, İsa Gökler, Azi̇z Türkoğlu
Macrofungi Of Bekilli (Denizli) District, Saci̇t Köse, Kudret Gezer, İsa Gökler, Azi̇z Türkoğlu
Turkish Journal of Botany
The material of this study comprises macrofungi specimens collected from different localities in Bekilli (Denizli) in 1998 and 1999. As a result of field and laboratory studies, 61 taxa belonging to 2 classes (Ascomycetes and Basidiomycetes) and 28 families are reported.
Mms-Induced Cytomixis In Pollen Mother Cells Of Broad Bean (Vicia Faba L.), Tariq Ahmad Bhat, Sahba Parveen, Ainul Haq Khan
Mms-Induced Cytomixis In Pollen Mother Cells Of Broad Bean (Vicia Faba L.), Tariq Ahmad Bhat, Sahba Parveen, Ainul Haq Khan
Turkish Journal of Botany
Cytomixis was recorded during microsporogenesis in broad bean (Vicia faba L.), a member of the family Fabaceae. It is an important pulse crop used as vegetable, silage, forage and stock feed. The phenomenon of cytomixis was observed in various stages of meiosis in methyl methane sulphonate (MMS) treated populations of Vicia faba L. Cytomixis was observed to occur through various methods, i.e. by forming cytoplasmic channels, and direct fusion of pollen mother cells (PMCs), the former was more frequent than the latter. Both types were observed at different stages of microsporogenesis. The migration of nuclear content involved all the chromatin/chromosomes …
Check-List Of Additional Taxa To The Supplement Flora Of Turkey Iii, Neri̇man Özhatay, Şükran Kültür
Check-List Of Additional Taxa To The Supplement Flora Of Turkey Iii, Neri̇man Özhatay, Şükran Kültür
Turkish Journal of Botany
This checklist contains a total of 295 taxa published as additions to the Flora of Turkey. It includes details of taxa neither recorded by P.H.Davis' "Flora of Turkey and the East Aegean Islands" or its supplements, nor in the 2 previously published checklists. The records in this list are based on data given in nearly 195 papers published up to the end of 2004.
Teucrium Chasmophyticum Rech. F. (Lamiaceae): A New Record For The Flora Of Turkey, Ali̇ Arslan Dönmez
Teucrium Chasmophyticum Rech. F. (Lamiaceae): A New Record For The Flora Of Turkey, Ali̇ Arslan Dönmez
Turkish Journal of Botany
An interesting chasmophytic Teucrium specimen was collected from Siirt, SE Turkey. It was identified according to the Flora of Turkey as T. paederotoides but with some doubt about certain characters. It was identified as T. chasmophyticum without any doubt from Flora Iranica. Because of some indistinct points between these 2 taxa, the recently described taxon was re-evaluated. The type specimen of the taxon was examined at W herbarium and its revised description is given here. A photograph of the type specimen and the Turkish material taken from the field is supplied along with a map.
Net Planktonic Diatom (Bacillariophyceae) Composition Of Lake Abant (Bolu), Abuzer Çelekli̇, Okan Külköylüoğlu
Net Planktonic Diatom (Bacillariophyceae) Composition Of Lake Abant (Bolu), Abuzer Çelekli̇, Okan Külköylüoğlu
Turkish Journal of Botany
The diatom flora of Lake Abant was studied monthly for 2 years (June 2003-June 2005) from 3 littoral and 2 vertical stations. A total of 123 diatom taxa were identified. Some species (Asterionella formosa Hassall, Cymbella silesiaca Bleisch, C. cistula Kirchner, Fragilaria biceps (Kützing) Lange-Bertalot, F. dilata (Brebisson) Lange-Bertalot, Navicula radiosa Kützing, and Rhopalodia gibba Müller) showed frequent occurrence in each month at all stations. Numbers of diatoms were increased in certain months in autumn, i.e. November (83 taxa) and December (68 taxa) 2003, and August (65 taxa) and September (76 taxa) 2004.
Net Diatom (Bacillariophyceae) Flora Of Lake Gölköy (Bolu), Abuzer Çelekli̇
Net Diatom (Bacillariophyceae) Flora Of Lake Gölköy (Bolu), Abuzer Çelekli̇
Turkish Journal of Botany
The diatom flora of Lake Gölköy was studied monthly over 2 years (June 2003-June 2005) from 3 littoral and 2 vertical stations in Lake Gölköy (Bolu, Turkey). A total of 119 diatom taxa were identified, most of them belonging to Naviculaceae (48.7%) Kützing, Fragilariceae (16.8%) Hustedt, Surirellaceae (11.8%) Kützing, and Bacillariaceae (6.7%) Ehrenberg, from which many species (Asterionella formosa Hassall, Aulacoseria granulata (Ehrenberg) Simonsen, Cyclotella praetermis Lund, Cymbella cistula Kirchner, Fragilaria biceps (Kützing) Lange-Bertalot, F. crotonensis Kitton, F. dilata (Brebisson) Lange-Bertalot, Navicula radiosa Kützing, and Nitzschia sigmoidae (Nitzsch) Smith) were found each month at all stations. Species richness was especially …
Subalpine And Alpine Flora Of Altındere Valley (Maçka, Trabzon), Seyran Palabaş Uzun, Rahi̇m Anşi̇n
Subalpine And Alpine Flora Of Altındere Valley (Maçka, Trabzon), Seyran Palabaş Uzun, Rahi̇m Anşi̇n
Turkish Journal of Botany
The subalpine and alpine flora of Altındere Valley (Maçka, Trabzon) was investigated during 2000-2002. The study area is situated in the Colchis sector of the Euro-Siberian floristic area of the Holarctic region with regard to plant geography. In this area, 384 plant taxa belonging to 61 families and 190 genera were determined. The largest family is Asteraceae (39; 10%), and the second largest is Rosaceae (35; 9%). The rates of the taxa belonging to certain phytogeographical regions are as follows: 50.27% Euro-Siberian, 5.99% Irano-Turanian and 1.04% Mediterranean. Moreover, phytogeographically unknown taxa are 42.70%. Thirty-two endemic (8.3%) and 9 rare (2.3%) …
Foliar Response Of Ipomea Pes-Tigridis L. To Coal-Smoke Pollution, Rama Bharti Verma, -- Mahmooduzzafar, Tariq Omar Siddiqi, Muhammad Iqbal
Foliar Response Of Ipomea Pes-Tigridis L. To Coal-Smoke Pollution, Rama Bharti Verma, -- Mahmooduzzafar, Tariq Omar Siddiqi, Muhammad Iqbal
Turkish Journal of Botany
Air pollutants emitted from Badarpur Thermal Power Plant, New Delhi, influenced the foliar features of a common weed, Ipomea pes-tigridis L., as observed at distances 0.5, 2, 6, 12 and 16 km from the emission source. Observations recorded at the pre-flowering, flowering and post-flowering stages of plant growth indicated that the stomatal pore size, number of stomata, and stomatal index on both surfaces of leaves decreased near the source of pollution. Compared with the control, chlorophyll a, b and carotenoid contents decreased significantly under pollution stress. Rate of photosynthesis and stomatal conductance dropped drastically, whereas CO_2 level in the mesophyll …
Microfungi Isolated From Plants In Kahramanmaraş Province, Turkey, Zeli̇ha Bahçeci̇oğlu, Şanli Kabaktepe, Bayram Yildiz
Microfungi Isolated From Plants In Kahramanmaraş Province, Turkey, Zeli̇ha Bahçeci̇oğlu, Şanli Kabaktepe, Bayram Yildiz
Turkish Journal of Botany
Herein, 146 microfungi species and their hosts recorded in Kahramanmaraş province are given with their localities. The research was carried out between 2000 and 2004. Approximately 1821 vascular plant specimens infected and uninfected with microfungi were collected from the area. In total, 146 microfungi species were identified on the collected host specimens. These species belong to 36 genera and 17 families; 43 species belong to Ascomycota, 94 to Basidiomycota, 1 to Oomycota, and 8 to Mitosporic Fungi (Deuteromycota). Five microfungi are given at the generic level, 16 species are new records for Turkey, and 4 new hosts were recorded for …
Seed Morphology And Seed Coat Sculpturing Of Epilobium L. Species (Onagraceae Juss.) From Iran, Reza Sheikh Akbari, Dina Azizian
Seed Morphology And Seed Coat Sculpturing Of Epilobium L. Species (Onagraceae Juss.) From Iran, Reza Sheikh Akbari, Dina Azizian
Turkish Journal of Botany
The seeds of 32 specimens belonging to 15 species from 2 sections of the genus Epilobium L. (Onagraceae Juss.) were studied by both a light and scanning electron microscope (SEM). Seed morphology of the examined specimens exhibits some variation in shape and size. Seed size ranges between 0.97 mm and 2.2 mm in length. They are ovate, oblong-ovate, and narrowly ovate (ovate-attenuate). The results of SEM investigation of seed coat sculpturing also revealed 5 distinct types of surface structure of the seeds, which are mainly correlated to external morphology of the species.
Myxomycetes Of Kestel Forest (Kadınhanı, Konya), Gönül Demi̇rel, Giyasetti̇n Kaşik, Celaleddi̇n Öztürk
Myxomycetes Of Kestel Forest (Kadınhanı, Konya), Gönül Demi̇rel, Giyasetti̇n Kaşik, Celaleddi̇n Öztürk
Turkish Journal of Botany
The samples of Myxomycetes recorded in this study were collected from Kestel forest (Kadınhanı, Konya) in 2003 and 2004. As a result of field and laboratory studies, 7 families, and 32 taxa belonging to 11 genera were determined. Eight of these were collected in their natural habitat while 24 were isolated in moist chamber culture.
Isolation Of Dna For Sequence Analysis From Herbarium Material Of Some Lichen Specimens, Sümer Aras, Demet Cansaran Duman
Isolation Of Dna For Sequence Analysis From Herbarium Material Of Some Lichen Specimens, Sümer Aras, Demet Cansaran Duman
Turkish Journal of Botany
An improved protocol for the isolation of DNA from herbarium material of some lichen specimens is described. The isolated DNA is suitable for PCR reactions for DNA sequence analysis. The hexadecyltrimethylammonium bromide (CTAB) based protocol defined in this study provides a number of advantages, mainly speed and reliability. In addition, different DNA extraction protocols were examined to determine the yield of DNA from the thallus of lichen specimens. The methods examined include a CTAB based protocol and a sodium dodecyl sulphate (SDS) based protocol. Although these procedures yielded DNA of suitable purity for PCR analysis, the improved protocol yielded DNA …