Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Arabidopsis thaliana (3)
- Arabidopsis (2)
- Fertilizer (2)
- Actin (1)
- Amino acid biosynthesis (1)
-
- Bacterial wilt (1)
- Biocontrol agent (1)
- Biosolid (1)
- Carbohydrates (1)
- Carrots (1)
- Contaminants of emerging concern (1)
- Conventional tillage (1)
- DAHP synthase (1)
- DHD-SHD (1)
- DHQS (1)
- Environmental stress (1)
- Extraction (1)
- Field stress (1)
- Forward genetic screen (1)
- Germination (1)
- Group Contribution (1)
- In vitro motility assay (1)
- Interrill erosion (1)
- Lignin (1)
- Limiting (1)
- Live cell imaging (1)
- Meloidgyne incognita (1)
- Metabolism (1)
- Metabolomics (1)
- Myosin (1)
Articles 1 - 13 of 13
Full-Text Articles in Life Sciences
Expression Of Carbohydrates Biosynthetic Genes In Developing Soybean Seeds, Jayden Rosen, Karen A. Hudson
Expression Of Carbohydrates Biosynthetic Genes In Developing Soybean Seeds, Jayden Rosen, Karen A. Hudson
The Summer Undergraduate Research Fellowship (SURF) Symposium
An essential part of livestock diets is soybean meal, which is a major source of protein, but which also consists of antinutritional carbohydrates. Antinutritional carbohydrates such as raffinose and stachyose lead to irritation to the gut for monogastric livestock as well as unhealthy weight gain. A major objective of soybean genetics is to reduce these antinutritional carbohydrates within the seed and increase the levels of good carbohydrates. This will lead to healthier livestock and better meat quality. To select genes potentially responsible for variation in carbohydrate levels in seeds, the expression of genes encoding several biosynthetic enzymes was measured during …
Another Route For Amino Acid Production?: Reverse Genetic Probing For A Functional Cytosolic Shikimate Pathway In Plants, Gabrielle C. Buck, Joseph Lynch, Natalia Dudareva
Another Route For Amino Acid Production?: Reverse Genetic Probing For A Functional Cytosolic Shikimate Pathway In Plants, Gabrielle C. Buck, Joseph Lynch, Natalia Dudareva
The Summer Undergraduate Research Fellowship (SURF) Symposium
The shikimate pathway is a metabolic pathway that produces the three aromatic amino acids—phenylalanine, tryptophan, and tyrosine—which are essential to human diets and necessary for many plant functions. Consequently, the shikimate pathway is commonly targeted for antibiotic and herbicide strategies as well as genetic engineering in several fields. This pathway is known to be localized in the plastids, or double membrane-bound organelles, of plant cells; however, there is enzymatic evidence of another shikimate pathway in the cell fluid, or cytosol. To determine whether a complete cytosolic shikimate pathway exists, we used a modified gene for the first enzyme of the …
Increasing Maize Tolerance To Drought And Flood With Seed Coating Treatments, Jacob E. Bennett, Achint Sanghi, R. P. Kingsly Ambrose
Increasing Maize Tolerance To Drought And Flood With Seed Coating Treatments, Jacob E. Bennett, Achint Sanghi, R. P. Kingsly Ambrose
The Summer Undergraduate Research Fellowship (SURF) Symposium
The lack of irrigation in regions prone to drought, and flooding due to high rainfall or lack of drainage affects seed viability and the subsequent germination and crop establishment. Seed treatment in the form of coatings shows promise as an effective method to preserve the viability of corn (Zea mays) seeds in drought and flood conditions. Chemical formulations may help improve the seed corn vigor under these stressed conditions. This study examined the efficacy of β-aminobutyric acid [BABA] and N-isopropylacrylamide [NIPA] in inducing drought resistance, as well as the ability of lanolin and linseed oil to provide flood …
In Vitro Motility Of Actin Filaments Powered By Plant Myosins Xi, Cesar A. Quintana-Cataño, Christopher J. Staiger, Weiwei Zhang
In Vitro Motility Of Actin Filaments Powered By Plant Myosins Xi, Cesar A. Quintana-Cataño, Christopher J. Staiger, Weiwei Zhang
The Summer Undergraduate Research Fellowship (SURF) Symposium
The actomyosin network is thought to support fundamental processes of plant development and cell expansion such as polarized elongation of root hairs and the diffuse growth of epidermal and mesophyll cells. Inhibition of myosins via pharmacological treatments represents one of the key approaches for understanding of their roles in different cellular processes. However, the use of the standard plant myosin inhibitor, 2,3-butanedionemonoxime (BDM), is questioned as it requires a high concentration and may not be as specific as desired. By testing drugs that inhibit animal and yeast myosins V, the Staiger laboratory previously found pentabromopseudilin (PBP) as a potential inhibitor …
Thermodynamic Analysis Of Phenylpropanoid Pathway In Arabidopsis Thanliana, Patrick J. Ioerger, Rohit Jaini, John A. Morgan
Thermodynamic Analysis Of Phenylpropanoid Pathway In Arabidopsis Thanliana, Patrick J. Ioerger, Rohit Jaini, John A. Morgan
The Summer Undergraduate Research Fellowship (SURF) Symposium
Biofuels represent a renewable alternative to traditional fossil fuels. As dependence on fossil fuels rise so does the importance of improving the production of alternative fuels. Lignin poses one obstacle in the development of such alternative fuels. Its presence strengthens cell walls and hinders degradation of polysaccharides into monosaccharides, increasing cost and time while decreasing efficiency of the process. Lignin is composed of three monolignols, each of which is produced through the Phenylpropanoid pathway; a series of chemical reactions. This work aims to determine which reactions in the pathway are least thermodynamically favorable and thus most limiting. From metabolic mapping …
You Don’T Have To Go Home But You Carrot Stay Here: Root-Knot Nematode Biological Control In Carrots, Casey R. Martin, Sahar Abdelrazek, Lori A. Hoagland
You Don’T Have To Go Home But You Carrot Stay Here: Root-Knot Nematode Biological Control In Carrots, Casey R. Martin, Sahar Abdelrazek, Lori A. Hoagland
The Summer Undergraduate Research Fellowship (SURF) Symposium
Root-knot nematodes cause tens of billions of dollars in damages annually in agricultural production. The enormous economic losses brought about by nematodes, combined with the downsides to current methods for handling them, have led to an increased focus in understanding natural plant defense mechanisms and finding alternative methods to lessen the damage done by root-knot nematodes. Endophyte biocontrol agents such as Trichoderma harzianum have shown promise in reducing nematode damage. Recent studies have demonstrated that soil management practices and plant genotype can influence endophyte community structure, but it is unclear whether such differences influence susceptibility to nematode damage. This study …
Long-Term Tillage System Impacts On Soil Erodibility, Julianne R. Chechanover, Dennis C. Flanagan
Long-Term Tillage System Impacts On Soil Erodibility, Julianne R. Chechanover, Dennis C. Flanagan
The Summer Undergraduate Research Fellowship (SURF) Symposium
Conservation tillage practices, such as no-till agriculture, have the potential of reducing the erodibility of a soil compared to conventional agricultural practices. This research sought to determine whether long-term agricultural practices affect the baseline erodibility properties of a soil. Two soils from Throckmorton-Purdue Agricultural Center in Tippecanoe County, Indiana were used during this experiment. One soil was treated with a long-term conventional tillage (fall chisel, spring disk) system and the other soil was treated with a long-term no-till system. The soils’ interrill erodibility, and rill erodibility and critical hydraulic shear stress were measured under a rainfall simulator using soil boxes …
Detecting Genomic Regions Responsible For Resistance In Arabidopsis, Valeria Cancino, Anjali Iyer-Pascuzzi, Rucha Karve
Detecting Genomic Regions Responsible For Resistance In Arabidopsis, Valeria Cancino, Anjali Iyer-Pascuzzi, Rucha Karve
The Summer Undergraduate Research Fellowship (SURF) Symposium
Ralstonia solanacearum is a soil-borne plant root colonizing pathogen and the casual agent of bacterial wilt (BW) disease. BW leads to severe yield loss in a wide variety of agricultural commodity crops, such as tomato, banana, and pepper. In this study, we look at the plant-pathogen interaction between Ralstonia solanacearum and various ecotypes of Arabidopsis thaliana with the goal of finding resistant ecotypes. To identify resistant ecotypes, seeds are first sterilized and left to soak in the dark. Then the seeds are plated on agar media, transferred to a growth chamber, and allowed to grow for 5 days. On day …
Using Stable Isotopes To Quantify Nitrogen Fates In Container Plants, Sam Raimann, Greg Michalski, Michael V. Mickelbart
Using Stable Isotopes To Quantify Nitrogen Fates In Container Plants, Sam Raimann, Greg Michalski, Michael V. Mickelbart
The Summer Undergraduate Research Fellowship (SURF) Symposium
Currently, in the agriculture field, it is not yet known the accurate amount of Nitrogen in fertilizer that plants take up. This statistic, known as the Nitrogen Use Efficiency is currently known to be within the 30-50% range (Lea-Cox and Ross, 2001). This is very important figure to know and it is a figure that can be improved, and therefore much time, energy, and resources can be saved. This research project will use concepts involving stable isotopes to examine red maple plant material and the soilless media that the plants were grown in. Three different isotope-labelled fertilizer treatments will be …
Evaluating Contaminants Of Emerging Concern In Commercial Biosolid-Based Fertilizers, John Hemmerling, Michael L. Mashtare, Linda S. Lee
Evaluating Contaminants Of Emerging Concern In Commercial Biosolid-Based Fertilizers, John Hemmerling, Michael L. Mashtare, Linda S. Lee
The Summer Undergraduate Research Fellowship (SURF) Symposium
The production and popularity of commercially available biosolid-based fertilizers are increasing because of their economic, environmental, and plant nutrition benefits, particularly in urban and suburban areas. Because biosolid-based fertilizers are derived from waste water treatment plant residuals, we hypothesized that there is the potential for micropollutants to persist in these products. Their presence would be of particular concern due to their potential impact on human and ecological health and risk of bioaccumulation. This study involves quantifying contaminants of emerging concern in three biosolid-based fertilizers, and 2 non-biosolid-based fertilizers, a composted animal manure and an organic compost. Our extraction method employed …
Regulation Of Mor By Different Abiotic Stresses In Arabidopsis Thaliana, Luke Stepan, Rucha Karve, Anjali Iyer-Pascuzzi
Regulation Of Mor By Different Abiotic Stresses In Arabidopsis Thaliana, Luke Stepan, Rucha Karve, Anjali Iyer-Pascuzzi
The Summer Undergraduate Research Fellowship (SURF) Symposium
The climate is changing and as a consequence the environment is becoming hotter and drier. How different plants will react to these changes is unknown. Identification of genes involved in stress tolerance can help predict plant-environment interactions and lead to stress tolerant plants. The MOR gene (Modulator Of Root ROS, ROS = Reactive Oxygen Species) in the model plant Arabidopsis thaliana encodes a transcription factor that may regulate stress responses, as mor mutants are drought tolerant. We hypothesized that MOR expression changes in response to different abiotic stress stimuli. We tested MOR expression in response to salt (NaCl), abscisic acid …
Forward Genetic Screen Of Trichomes For Discovery Of Cytoskeleton-Based Mutants, Adam M. Fessenden, Samuel Belteton, Daniel B. Szymanski
Forward Genetic Screen Of Trichomes For Discovery Of Cytoskeleton-Based Mutants, Adam M. Fessenden, Samuel Belteton, Daniel B. Szymanski
The Summer Undergraduate Research Fellowship (SURF) Symposium
Understanding plant cell development and what genes influence cell growth can lead to breakthroughs in beneficial areas such as bioremediation, agricultural production, and biofuels. However, information on many of the genes that control plant cell growth is either unknown or severely limited. Further research to fully comprehend the genetic pathways within the cells will enable the genetic engineering of plants to further benefit society. One approach is the combined use of a forward genetic screen, sophisticated growth analysis, and gene identification. Using Arabidopsis thaliana trichomes, leaf hairs, as a model system, the mutagenized population of thousands of plants was screened …
Development Of A Metabolomic Method To Define The Phenylalanome In Arabidopsis Thaliana, Cole G. Wunderlich, Clint Chapple, Xu Li
Development Of A Metabolomic Method To Define The Phenylalanome In Arabidopsis Thaliana, Cole G. Wunderlich, Clint Chapple, Xu Li
The Summer Undergraduate Research Fellowship (SURF) Symposium
In the study of metabolomics, one of the greatest challenges can be accurately identifying compounds detected in biological extracts, especially when standards are not readily available. Current metabolomic methods are also limited in that they provide little to no information about a compound’s metabolic origin. In this study, we sought to address these issues by developing a novel metabolomic method that employs stable isotope feeding, LC-MS, Xcms, and an analytical software algorithm to study the ‘phenylalanome’ of Arabidopsis thaliana. Using this approach we were able to develop a method that, based on current results, is capable of detecting over …