Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Expression Of Holo And Apo Forms Of Spinach Acyl Carrier Protein-I In Leaves Of Transgenic Tobacco Plants, M. A. Post-Beittenmiller, Katherine M. Schmid, J. B. Ohlrogge
Expression Of Holo And Apo Forms Of Spinach Acyl Carrier Protein-I In Leaves Of Transgenic Tobacco Plants, M. A. Post-Beittenmiller, Katherine M. Schmid, J. B. Ohlrogge
Scholarship and Professional Work - LAS
Acyl carrier protein (ACP) is a chloroplast-localized cofactor of fatty acid synthesis, desaturation, and acyl transfer. We have transformed tobacco with a chimeric gene consisting of the tobacco ribulose-1,5-bisphosphate carboxylase promoter and transit peptide and the sequence encoding the mature spinach ACP-I. Spinach ACP-I was expressed in the transformed plants at levels twofold to threefold higher than the endogenous tobacco ACPs as determined by protein immunoblots and assays of ACP in leaf extracts. In addition to these elevated levels of the holo form, there were high levels of apoACP-I, a form lacking the 4'-phosphopantetheine prosthetic group and not previously detected …