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Full-Text Articles in Life Sciences
Validation Of A Diagnostic Marker For Primocane-Fruiting In Blackberry, Isabella Vaughn, Alexander Silva, Carmen Johns, Lacy Nelson, Margaret Worthington
Validation Of A Diagnostic Marker For Primocane-Fruiting In Blackberry, Isabella Vaughn, Alexander Silva, Carmen Johns, Lacy Nelson, Margaret Worthington
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Typical blackberries (Rubus subgenus Rubus) have perennial crowns and roots and biennial canes. The first-year canes (primocanes) are usually vegetative, while second-year canes (floricanes) produce fruit. Primocane-fruiting blackberries produce fruit on first-year canes and are desirable to growers because they potentially allow for a longer harvest season in temperate regions and enable production in tropical areas where no natural chill hours are accumulated. The development of molecular markers for desirable traits can potentially increase efficiency in blackberry breeding. However, to date, there are no diagnostic molecular markers for economically important traits in blackberries. Primocane-fruiting is recessively inherited, and tetraploid blackberries …
Efficiency Of The Nuclease I-Scei In Excising Selectable Marker Genes From The Plant Genome, Elliot E. Pruett, Soumen Nandy, Vibha Srivastava
Efficiency Of The Nuclease I-Scei In Excising Selectable Marker Genes From The Plant Genome, Elliot E. Pruett, Soumen Nandy, Vibha Srivastava
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Gene stacking is a method used in biotechnology by which multiple genes can be placed at a single genomic site, thereby simplifying plant breeding. In this approach, DNA nucleases are used for excising selectable marker genes (SMG), which are the unneeded components of transgenic plants. The goal of this project is to evaluate the effectiveness of the nuclease I-SceI in excising DNA in plants. Specifically, this study tests heat-inducible I-SceI through the use of a heat-shock promoter (HS) in order to control SMG excision by heat application. The DNA plasmid containing a visual marker gene flanked by I-SceI target sites …