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Full-Text Articles in Life Sciences
Purification And Characterization Of Syringacin 4-A, A Bacteriocin From Pseudomonas Syringae 4-A, William L. Haag, Anne K. Vidaver
Purification And Characterization Of Syringacin 4-A, A Bacteriocin From Pseudomonas Syringae 4-A, William L. Haag, Anne K. Vidaver
Department of Plant Pathology: Faculty Publications
Syringacin 4-A, a bacteriocin produced by Pseudomonas syrinagae 4-A, was obtained by induction with ultraviolet irradiation or mitomycin C. Approximately 1,000-fold purification of the bacteriocin was achieved by manganous chloride precipitation, differential centrifugation, and chromatography on hydroxyapatite columns. The purified syngacin was homogeneous on hydroxyapatite columns and sucrose density gradients; it also sedimented as a single entity in the analytical ultracentrifuge. The buoyant density of purified syringacin in cesium chloride was 1.294 g/ml. The sedimentation coefficient was calculated as 120S, and the diffusion coefficient was 6.49 x 10-8 cm2/s. The molecular weight was calculated as …
Influence Of Molecular Weights Of Bacteriophage Φ6 Double-Stranded Ribonucleic Acids On Interferon Induction, W.J. Kleinschmidt, James L. Van Etten, A. K. Vidaver
Influence Of Molecular Weights Of Bacteriophage Φ6 Double-Stranded Ribonucleic Acids On Interferon Induction, W.J. Kleinschmidt, James L. Van Etten, A. K. Vidaver
Department of Plant Pathology: Faculty Publications
The interferon-inducing capabilities of the three molecular segments of bacteriophage φ6 double-stranded ribonucleic acid increased with increasing molecular weight.
Base Composition And Hybridization Studies Of The Three Double-Stranded Rna Segments Of Bacteriophage Φ6, James L. Van Etten, A. K. Vidaver, R. K. Koski, J. P. Burnett
Base Composition And Hybridization Studies Of The Three Double-Stranded Rna Segments Of Bacteriophage Φ6, James L. Van Etten, A. K. Vidaver, R. K. Koski, J. P. Burnett
Department of Plant Pathology: Faculty Publications
The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage φ6 were isolated and shown to have similar melting temperatures and base compositions. RNA: RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of the large dsRNA segment. The two smaller RNA segments reanneal rapidly even at low temperatures; in contrast, the large dsRNA reannealed only at higher temperatures. Evidence is also presented which suggests that the dsRNAs may contain a short single-stranded RNA tail.
Physiological And Morphological Correlation Of Rhizopus Stolonifer Spore Germination, James L. Van Etten, Lee Bulla Jr., Grant St. Julian
Physiological And Morphological Correlation Of Rhizopus Stolonifer Spore Germination, James L. Van Etten, Lee Bulla Jr., Grant St. Julian
Department of Plant Pathology: Faculty Publications
Sporangiospores of Rhizopus stolonifer were examined at various stages of germination by scanning electron and phase-contrast microscopy. These observations were correlated with changes in spore dry weight, spore volume, respiration, and syntheses of ribonucleic acid, deoxyribonucleic acid, and protein during germination.
Survival Mechanisms Of Phytopathogenic Bacteria, M. L. Schuster, D. P. Coyne
Survival Mechanisms Of Phytopathogenic Bacteria, M. L. Schuster, D. P. Coyne
Department of Plant Pathology: Faculty Publications
Natural habitats usually do not provide bacteria the continuity of agricultural crops. With continuous culture. perpetuation of pathogen is no problem. Although agricul tural practices provide some discontinuity between crops. it is less than that in nature. Uniformity of crop germ plasm also favors inoculum buildup and perhaps perpetuation of the pathogens.
Corynebacterium Nebraskense, A New, Orange-Pigmented Phytopathogenic Species, Anne K. Vidaver, Manley Mandel
Corynebacterium Nebraskense, A New, Orange-Pigmented Phytopathogenic Species, Anne K. Vidaver, Manley Mandel
Department of Plant Pathology: Faculty Publications
A new species of Corynebacterium isolated from field corn is described. The isolates form a homogeneous group that is recognized as a new species, Corynebacterium nebraskense. The organism is characterized by orange-pig-mented colonies, inability to grow on 0.005% triphenyltetrazolium chloride agar, specific bacteriophage sensitivity, and a guanine plus cytosine content of 73.5 mol%. These and other characters differentiate this organism from other described phytopathogenic corynebacteria. The type strain of C. nebraskense is Fur-1 (= ATCC 27822 = NCPPB 2578).