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Full-Text Articles in Life Sciences
Identification And Characterization Of Isoflavone Reductase Family Members In Soybean, Negin Azizkhani
Identification And Characterization Of Isoflavone Reductase Family Members In Soybean, Negin Azizkhani
Electronic Thesis and Dissertation Repository
Soybean’s yield is threatened by Phytophthora sojae, a pathogen responsible for stem and root rot disease. Glyceollins, unique antimicrobial agents specific to soybeans in partially preventing P. sojae infection, are derived from the isoflavonoid branch of the general phenylpropanoid pathway. One pivotal enzyme exclusively involved in glyceollin synthesis in soybean is the isoflavone reductase (GmIFR), which catalyzes the 2'-hydroxydaidzein conversion to 2'-hydroxy-2,3-dihydrodaidzein as a precursor for glyceollin biosynthesis. To comprehensively identify all members of the GmIFR gene family within the soybean genome, keyword and blast protein searches were conducted, identifying 98 putative GmIFRs. Among these candidates, …
Inheritance, Differential Expression, And Candidate Gene Analyses For Avr2 In Phytophthora Sojae, Chelsea S. Ishmael
Inheritance, Differential Expression, And Candidate Gene Analyses For Avr2 In Phytophthora Sojae, Chelsea S. Ishmael
Electronic Thesis and Dissertation Repository
Phytophthora sojae is an oomycete responsible for seed, root and stem rot of soybean plants. Managing this disease relies on growing soybean cultivars with race-specific resistance (Rps) genes that deliver complete host immunity in the presence of corresponding pathogen avirulence (Avr) effector proteins. The aims of this study were to characterize virulence towards Rps2 among different P. sojae strains, track the inheritance of this trait, and attempt to identify an Avr2 gene. Fifteen P. sojae strains were tested for virulence towards Rps2 and crosses were performed between selected virulent and avirulent strains to follow the inheritance of virulence. …
Planting The Chalcone Reductase Family Tree: Identification And Characterization Of Chalcone Reductase Genes In Soybean, Caroline Julia Sepiol
Planting The Chalcone Reductase Family Tree: Identification And Characterization Of Chalcone Reductase Genes In Soybean, Caroline Julia Sepiol
Electronic Thesis and Dissertation Repository
Soybean (Glycine max [L.] Merr) is an important crop grown in Canada, generating $2.4 billion in sales. Though this number may be promising, soybean farmers lose about $50 million worth of yield annually due to root and stem rot disease caused by Phytophthora sojae. Many strategies have been developed to combat the infection; however, these methods are prohibitively expensive. A ‘cost effective’ approach to this problem is to select a trait naturally found in soybean that can increase resistance. One such trait is the increased production of root glyceollins. One of the key enzymes exclusively involved in glyceollin …
Inheritance Of Virulence In The Root Rot Pathogen Phytophthora Sojae, Sirjana Devi Shrestha
Inheritance Of Virulence In The Root Rot Pathogen Phytophthora Sojae, Sirjana Devi Shrestha
Electronic Thesis and Dissertation Repository
The oomycete Phytophthora sojae causes stem and root rot of soybean plants. The interaction of pathogen avirulence (Avr) and host resistance (R)-genes determine the disease outcome. The Avr3a mRNA transcript level is variable among P. sojae strains and determines virulence towards the R-gene Rps3a. To study the inheritance of virulence, genetic crosses and self-fertilizations were performed. A cross between P. sojae strains ACR10 and P7076 causes transgenerational gene silencing of Avr3a allele, and this effect is meiotically stable up to the F5 generation. However, test-crosses of F1 (Avr3aACR10/Avr3a …
Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma
Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma
Electronic Thesis and Dissertation Repository
Part of the resistance mechanism of soybean (Glycine max [L.] Merr.) to Phytopthora sojae Kauf. & Gerd. involves pre-formed root suberin. In order to investigate the role of suberin in this host-pathogen interaction, I characterized hairy roots, formed as a result of Agrobacterium rhizogenes (Riker et al.) Conn infection, as a model to be used as a reliable soybean transformation system. I established hairy root cultures and demonstrated that they were a result of A. rhizogenes infection. The anatomy and suberin deposition in soybean hairy roots was examined, and found to be very similar to that of wild-type roots. …