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Full-Text Articles in Life Sciences

Utilizing Rnai Technology To Develop Novel Agricultural Pesticides, Ethan B. Zand Aug 2022

Utilizing Rnai Technology To Develop Novel Agricultural Pesticides, Ethan B. Zand

Undergraduate Student Research Internships Conference

A general description of why RNAi based pesticide technology is a breakthrough technology able to target only pests while not harming others. This presentation gives a summary of RNAi technology, the current drawbacks of conventional pesticides, and our research on how RNAi can be used against the two-spotted spidermite; a polyphagic pest that causes significant financial damage to Canadian and worldwide agriculture


My Summer Working With Two-Spotted Spider Mites, Renée A. Smith Aug 2022

My Summer Working With Two-Spotted Spider Mites, Renée A. Smith

Undergraduate Student Research Internships Conference

Two-spotted spider mites are a polyphagous pest, capable of eating a magnitude of food crops which causes a large problem for Canadian agriculture. Their ability to consume various crops stems from their ability to adapt to various chemical defence mechanisms. This ability allows them to acquire resistance to many commonly used pesticides. This has resulted in large infections in Canadian farms with few options to prevent the pests from affecting crop yields. Take a look at my project if you'd like to see how the Grbic lab is working to combat this issue using genetic engineering techniques!


Fungal Co-Pathogenicity On Panax Quinquefolius, Andrea Ong Aug 2022

Fungal Co-Pathogenicity On Panax Quinquefolius, Andrea Ong

Undergraduate Student Research Internships Conference

American ginseng (Panax quinquefolius) is a perennial crop commonly grown for its medicinal properties. Ginseng replant disease (GRD) is a condition that prevents the re-cultivation of ginseng in previous ginseng gardens and is normally associated with extensive root rot caused by the fungus Ilyonectria mors-panacis. My project aims to investigate the relationship common GRD fungal pathogens and their pathogenicity on 1 year old ginseng seedlings.


A Bacterial Microbiome Analysis Of Solarized Ginseng Garden Soils, Anka Colo Aug 2022

A Bacterial Microbiome Analysis Of Solarized Ginseng Garden Soils, Anka Colo

Undergraduate Student Research Internships Conference

American ginseng (Panax quinquefolius) is a highly valued perennial crop grown for its roots during a four-year cultivation cycle. American ginseng is subject to ginseng replant disease (GRD) in which severe root rot develops in newly planted ginseng grown in a former ginseng garden. A common strategy to mitigating GRD is not available and techniques such as fumigation, fungicides, and biocontrol are ineffective, banned, or are slowly being phased out. Alternatively, soil solarization is a pre-plant technique used to treat soil to reduce disease inoculum and alter soil microbiomes. In summer 2019, a six-week soil solarization experiment was …


Optimization Of Dna Extraction From Difficult Sandy-Loam Soils, Anka Colo Aug 2021

Optimization Of Dna Extraction From Difficult Sandy-Loam Soils, Anka Colo

Undergraduate Student Research Internships Conference

Ginseng is a high value perennial crop grown for its roots. A four-year cultivation cycle is required to obtain marketable ginseng roots, during which a condition called ginseng replant disease (GRD) develops. GRD is characterized by a severe root rot disease, and prevents the growth of newly planted ginseng in a former ginseng garden1. Microbiome analysis of ginseng garden soil will help our understanding of the formation of GRD and its severity. However, extracting DNA from the sandy-loam soils required for ginseng cultivation is difficult. Commercial kits are either too expensive or not applicable to the large volumes of soil …


Using Wgcna For Comprehensive Understanding Of Genes Involved In Suberin Biosynthesis From Rna-Seq Data Analysis, Stephanie Wu Aug 2021

Using Wgcna For Comprehensive Understanding Of Genes Involved In Suberin Biosynthesis From Rna-Seq Data Analysis, Stephanie Wu

Undergraduate Student Research Internships Conference

Suberin is a heteropolymer comprising a cell wall-bound poly(phenolic) domain (SPPD) covalently linked to a poly(aliphatic) domain (SPAD) that is deposited between the cell wall and plasma membrane (Woolfson 2018). Previous studies have measured phenolic and/or fatty acid compounds in suberized tissues, and for many of these the biosynthesis is known, However, restricting analyses to only those biomolecules that are known does not yield new information about other genes that may be involved. This experiment serves to identify suberin associated gene clusters within Modules, based on an analysis of their expression patterns using Weighted Gene Correlation Network Analysis (WGCNA). Once …