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Ultrastructural Localization Of Solanidine In Potato Tubers, Shau-Ron Han

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Solanine, the green substance in potato, Solanum tuberosum L., tubers, is a toxic glycoalkaloid that is a potential human health hazard. To control the formation of this glycoalkaloid a greater understanding of its site of synthesis is needed. Labelling of solanidine, a direct precursor in the biosynthesis of solanine, with digitonin may indirectly locate the site of solanine synthesis in tubers. A study using ultrastructural cytochemical techniques was initiated to explore this possibility.

Sprouted tips and peridermal complex (periderm and cortex) tissue were fixed three different ways: (1) glutaraldehyde only, (2) osmium tetroxide and glutaraldehyde, and (3) glutaraldehyde-osmium tetroxide-digitonin mixture. …