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Functional Characterization Of Petiolule-Like Pulvinus (Plp) Gene In Abscission Zone Development In Medicago Truncatula And Its Application To Genetic Improvement Of Alfalfa, Juan Du, Shaoyun Lu, Maofeng Chai, Chuanen Zhou, Liang Sun, Yuhong Tang, Jaydeep Kolape, Zhaozhu Wen, Marjan Behzadirad, Tianxiu Zhong, Juan Sun, Yunwei Zhang, Zeng-Yu Wang Aug 2020

Functional Characterization Of Petiolule-Like Pulvinus (Plp) Gene In Abscission Zone Development In Medicago Truncatula And Its Application To Genetic Improvement Of Alfalfa, Juan Du, Shaoyun Lu, Maofeng Chai, Chuanen Zhou, Liang Sun, Yuhong Tang, Jaydeep Kolape, Zhaozhu Wen, Marjan Behzadirad, Tianxiu Zhong, Juan Sun, Yunwei Zhang, Zeng-Yu Wang

Department of Agronomy and Horticulture: Faculty Publications

Alfalfa (Medicago sativa L.) is one of the most important forage crops throughout the world. Maximizing leaf retention during the haymaking process is critical for achieving superior hay quality and maintaining biomass yield. Leaf abscission process affects leaf retention. Previous studies have largely focused on the molecular mechanisms of floral organ, pedicel and seed abscission but scarcely touched on leaf and petiole abscission. This study focuses on leaf and petiole abscission in the model legume Medicago truncatula and its closely related commercial species alfalfa. By analysing the petiolule-like pulvinus (plp) mutant in M. truncatula at phenotypic level (breakstrength and shaking …


Functional Characterization Of Petiolule-Like Pulvinus (Plp) Gene In Abscission Zone Development In Medicago Truncatula And Its Application To Genetic Improvement Of Alfalfa, Juan Du, Shaoyun Lu, Maofeng Chai, Chuanen Zhou, Liang Sun, Yuhong Tang, Jin Nakashima, Jaydeep Kolape, Zhaozhu Wen, Marjan Behzadirad, Tianxiu Zhong, Juan Sun, Yunwei Zhang, Zeng-Yu Wang Jan 2020

Functional Characterization Of Petiolule-Like Pulvinus (Plp) Gene In Abscission Zone Development In Medicago Truncatula And Its Application To Genetic Improvement Of Alfalfa, Juan Du, Shaoyun Lu, Maofeng Chai, Chuanen Zhou, Liang Sun, Yuhong Tang, Jin Nakashima, Jaydeep Kolape, Zhaozhu Wen, Marjan Behzadirad, Tianxiu Zhong, Juan Sun, Yunwei Zhang, Zeng-Yu Wang

Nebraska Center for Biotechnology: Faculty and Staff Publications

Alfalfa (Medicago sativa L.) is one of the most important forage crops throughout the world. Maximizing leaf retention during the haymaking process is critical for achieving superior hay quality and maintaining biomass yield. Leaf abscission process affects leaf retention. Previous studies have largely focused on the molecular mechanisms of floral organ, pedicel and seed abscission but scarcely touched on leaf and petiole abscission. This study focuses on leaf and petiole abscission in the model legume Medicago truncatula and its closely related commercial species alfalfa. By analysing the petiolule-like pulvinus (plp) mutant in M. truncatula at phenotypic level (breakstrength and …


Establishment Of Perennial Legumes With An Annual Warm-Season Grass As A Companion Crop, Martina N. La Vallie May 2019

Establishment Of Perennial Legumes With An Annual Warm-Season Grass As A Companion Crop, Martina N. La Vallie

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The yields of perennial forage legumes are often hindered during the establishment year due to slow germination rates and weed competition. This study was conducted to determine if sorghum-sudangrass (Sorghum bicolor x S. bicolor var. sudanese) is a compatible annual companion crop for increased forage production, weed suppression, and legume establishment. In 2016, sorghum-sudangrass was paired with alfalfa (Medicago sativa L. ‘Ranger’), birdsfoot trefoil (Lotus corniculatus L.), Illinois bundleflower [Desmanthus illinoensis (Michx.) MacMill. ex B.L. Rob. & Fernald], purple prairie clover (Dalea purpurea Vent.), red clover (Trifolium pratense L.), and roundhead lespedeza (Lespedeza capitata Michx.). We studied effects of a …


Effects Of Aphid (Homoptera) Abundance And Surrounding Vegetation On The Encounter Rate Of Coccinellidae (Coleoptera), Chrysopidae (Neuroptera), And Nabidae (Hemiptera) In Alfalfa, James C. Kriz, Stephen D. Danielson, James R. Brandle, Erin E. Blankenship, Geoff M. Henebry Jul 2006

Effects Of Aphid (Homoptera) Abundance And Surrounding Vegetation On The Encounter Rate Of Coccinellidae (Coleoptera), Chrysopidae (Neuroptera), And Nabidae (Hemiptera) In Alfalfa, James C. Kriz, Stephen D. Danielson, James R. Brandle, Erin E. Blankenship, Geoff M. Henebry

Department of Entomology: Faculty Publications

Predaceous insect encounter rate was measured in 21 southeast Nebraska alfalfa fields through weekly sweep net sampling during 2002-03. The most frequently encountered predaceous insect families were Coccinellidae (Coleoptera), Nabidae (Hemiptera), and Chrysopidae (Neuroptera). The study used multiple regression analysis to examine the effect of aphid abundance and the surrounding vegetative patch composition on predaceous insect abundance. In 2002, Hippodamia parenthesis Say was encountered more frequently in fields with lower aphid abundances, and Hippodamia convergens Guerin-Meneville, Coccinella septempunctata L., and Nabis americoferus Carayon were encountered more frequently in fields with higher aphid abundances. In 2003, Coleomegilla maculata DeGeer was encountered …


Enhanced Bioremediation Of Soil Containing 2,4-Dinitrotoluene By A Genetically Modified Sinorhizobium Meliloti, Sisir K. Dutta, Gail P. Hollowell, Fawzy M. Hashem, L. David Kuykendall Jan 2003

Enhanced Bioremediation Of Soil Containing 2,4-Dinitrotoluene By A Genetically Modified Sinorhizobium Meliloti, Sisir K. Dutta, Gail P. Hollowell, Fawzy M. Hashem, L. David Kuykendall

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The symbiotic nitrogen-fixing soil bacterium, Sinorhizobium meliloti, is well known for its ability to interact with the leguminous plant Medicago sativa L. It has, however, not been reported that this species possesses the capability to degrade toxic nitroaromatic compounds, such as 2,4-dinitrotoluene (DNT) which is commonly associated with the degradation of the explosive trinitrotoluene (TNT). In this study, the pJS1 DNT-biodegradative plasmid was genetically transferred to S. meliloti strain USDA 1936, which was confirmed by plasmid profile analysis. Several standard analytical and chemical tests including high performance liquid chromatography (HPLC), nitrite (NO2) release assays, rhizosphere population and plant greenhouse …


Pesticide Use On Crops In Nebraska - 1987, Maurice Baker, Nancy Peterson, Shripat T. Kamble Aug 1990

Pesticide Use On Crops In Nebraska - 1987, Maurice Baker, Nancy Peterson, Shripat T. Kamble

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

This is the third and most comprehensive study of pesticide use on crops in Nebraska. The first was completed in 1978 and the second one in 1982. The first study indicated that approximately 25 million pounds (11.34 million kg) of active ingredients were used on the major crops in Nebraska. This increased to approximately 30.2 million pounds (13.7 million kg) in 1982. The USDA requires accurate information to meet their responsibilities. Thus, this survey was undertaken to determine: 1) the use of pesticides on crops, pasture and rangeland and 2) to identify pest management practices.


Alfalfa Insect Management Studies 1971-77, G. R. Manglitz, W. R. Kehr, D. L. Keith, J. M. Mueke, J. B. Campbell, R. L. Ogden, T. P. Miller Aug 1980

Alfalfa Insect Management Studies 1971-77, G. R. Manglitz, W. R. Kehr, D. L. Keith, J. M. Mueke, J. B. Campbell, R. L. Ogden, T. P. Miller

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Three tests in southwestern Nebraska during 1971 and 1972 evaluated insecticides against the army cutworm. Adult alfalfa weevils did not damage new second growth alfalfa in a small plot study during a 3-year period (1973-1975) at Gothenburg, NE. However, excellent control of larval alfalfa weevils was obtained. These results indicated a need to establish economic threshold levels for the alfalfa weevil in Nebraska to prevent unnecessary use of insecticides. Four tests to control the alfalfa weevil with registered insecticides verified the efficacy of these materials under Nebraska conditions. A series of tests conducted during 1975 at the Mead Field Laboratory …


Sulfur Fertilization Of Alfalfa And Corn On The Sandy Soils Of Nebraska, G. W. Rehm Jul 1978

Sulfur Fertilization Of Alfalfa And Corn On The Sandy Soils Of Nebraska, G. W. Rehm

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Objectives of these studies were: 1. To determine the effect of various S fertilizers and rates of S application on the yield of both alfalfa and corn grown on sandy soils. 2. To determine the residual effects of a single application of S fertilizer on the production of alfalfa. 3. To measure the influence of S fertilizers and the rate of S applied on the S content of corn and alfalfa tissue and S uptake by these two crops. 4. To develop a soil testing procedure which accurately predicts the requirement for the application of S fertilizers to alfalfa and …


Performance Of Certified Seed Lots Of Dawson Alfalfa, W. R. Kehr, G. R. Manglitz Aug 1976

Performance Of Certified Seed Lots Of Dawson Alfalfa, W. R. Kehr, G. R. Manglitz

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Breeder, foundation, and certified seed lots of Dawson alfalfa, Medicago sativa L., were tested to determine stability in performance during three generations of seed increase under certification. Two field experiments were conducted, a seeded forage yield test and a space-planted test. Greenhouse experiments included separate tests for resistance to pea aphids, spotted alfalfa aphids, and bacterial wilt. Results obtained on certified Dawson alfalfa seed classes in field and greenhouse experiments were in agreement with the original variety description.


Management Of Four Alfalfa Varieties To Control Damage From Potato Leafhoppers, W. R. Kehr, R. L. Ogden, J. D. Kindler Dec 1975

Management Of Four Alfalfa Varieties To Control Damage From Potato Leafhoppers, W. R. Kehr, R. L. Ogden, J. D. Kindler

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

The main purpose of this study was to obtain information on forage yield and quality of four alfalfa (Medicago sativa L.) varieties that differed in level of resistance to potato leafhopper (Empoasca fabae Harris) yellowing, when cut at three stages of growth in the second and third cuttings in field plots, with and without insecticide application. The stages of growth were bud, 1/10, and full bloom. Other purposes included study of the same varieties in field cages manually infested at 20, 40, and 60 adult leafhoppers/square yard, and in supplemental cuttings of field plots under high natural infestation …


Appraisal For Combined Pea Aphid And Spotted Alfalfa Aphid Resistance In Alfalfa, W. L. Howe, W. R. Kehr, C. O. Calkins Feb 1965

Appraisal For Combined Pea Aphid And Spotted Alfalfa Aphid Resistance In Alfalfa, W. L. Howe, W. R. Kehr, C. O. Calkins

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Sources of combined resistance to spotted alfalfa and pea aphids were found in Ranger alfalfa and its parental strains, foreign plant introductions, clones and their progenies. Ranger alfalfa appeared to be a good source for selection of plants with resistance to both aphids.


Sulfur Fertilizers For Alfalfa Production In Nebraska, R. L. Fox, A. D. Flowerday, F. W. Hosterman, H. F. Rhoades, R. A. Olson Jan 1964

Sulfur Fertilizers For Alfalfa Production In Nebraska, R. L. Fox, A. D. Flowerday, F. W. Hosterman, H. F. Rhoades, R. A. Olson

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

In order to elaborate all aspects of the sulfur problem in alfalfa production, a series of field, greenhouse and laboratory experiments was initiated to determine: 1. The influence of sulfur fertilization on the yield of alfalfa in Nebraska. 2. The influence of sulfur fertilization on the protein, vitamin A, and sulfur contents of alfalfa. 3. The sulfur supplying capacities of various soils under field and greenhouse conditions. 4. The sulfur content of precipitation at widely scattered points in Nebraska. 5. Soil properties associated with sulfur deficiency. 6. Optimum methods for estimating the sulfur status of soils by soil testing. The …


Performance Of Alfalfas Under Five Management Systems, W. R. Kehr, E. C. Conard, M. A. Alexander, F. G. Owen Jul 1963

Performance Of Alfalfas Under Five Management Systems, W. R. Kehr, E. C. Conard, M. A. Alexander, F. G. Owen

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

This bulletin reports stands, forage yields, and other agronomic data on alfalfas of diverse origin or type tested under five management systems at Lincoln, Nebraska. Narrow-crowned and broad-crowned types were tested. The management systems involved non-irrigated alfalfa-bromegrass tests (a) continuously grazed with steers, (b) cut for hay, and (c) rotationally grazed with sheep; an irrigated alfalfa-bromegrass test rotationally grazed with dairy cattle; and irrigated tests of alfalfas in pure stands cut for hay.


Studies Of The Mechanisms And Sources Of Spotted Alfalfa Aphid Resistance In Ranger Alfalfa, W. L. Howe, W. R. Kehr, M. E. Mcknight, G. R. Manglitz Mar 1963

Studies Of The Mechanisms And Sources Of Spotted Alfalfa Aphid Resistance In Ranger Alfalfa, W. L. Howe, W. R. Kehr, M. E. Mcknight, G. R. Manglitz

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Mechanisms and sources of spotted alfalfa aphid resistance in Ranger alfalfa and its five parental strains were investigated during 1957-1960.


Relationship Of Self-Fertility, Pollen Abortion, And Micronuclei Number To Agronomic Performance In Alfalfa, P. P. Rotar, W. R. Kehr Feb 1963

Relationship Of Self-Fertility, Pollen Abortion, And Micronuclei Number To Agronomic Performance In Alfalfa, P. P. Rotar, W. R. Kehr

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

The purpose of this investigation was to study self-fertility, pollen abortion, and meiosis in a randomly selected population of Ranger alfalfa clones and their polycross progenies, and to relate such information to agronomic data obtained on the same materials.


Origin And Performance Of Ranger Alfalfa, W. R. Kehr May 1959

Origin And Performance Of Ranger Alfalfa, W. R. Kehr

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Ranger alfalfa was developed through the cooperative efforts of personnel of the United States Department of Agriculture and the Nebraska Agricultural Experiment Station and released for seed increase in 1940. It is a winter-hardy, bacterial wilt resistant variety synthesized from five basic strains selected from Cossack, Turkistan and Ladak. Breeding methods used in the development of this variety were described in detail. Research results obtained on the performance of the basic strains and on the seed lots, produced under certification procedures, in controlled wilt and cold tests and forage yield trials under field conditions at the Nebraska station were presented …


Hairy Vetch For Nebraska, T. H. Goodding Feb 1951

Hairy Vetch For Nebraska, T. H. Goodding

Nebraska Agricultural Experiment Station: Historical Circulars

Hairy vetch is a winter annual legume. It may be planted either in the spring or fall. Hairy vetch often succeeds on soils where sweet clover and alfalfa fail. It is more tolerant to acid (lime-deficient) soils than most leguminous crops.


Ec1551 Insect Control In The Production Of Alfalfa Seed, Ephriam Hixson Jun 1947

Ec1551 Insect Control In The Production Of Alfalfa Seed, Ephriam Hixson

University of Nebraska-Lincoln Extension: Historical Materials

Extension circular 1551 discusses insect control in the production of alfalfa seed.


Alfalfa Breeding, H. M. Tysdal, T. A. Kiesselbach, H. M. Westover Jun 1942

Alfalfa Breeding, H. M. Tysdal, T. A. Kiesselbach, H. M. Westover

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Interest in the principles of alfalfa breeding has increased greatly with the growing "forage consciousness" of the country at large and the more specific interest developed through cooperation in the Alfalfa Improvement Conference. As is true of many other forage crops, information on the breeding behavior of alfalfa is meager. During the past few years, however, the genetics and principles of reproduction of this crop have come to be better understood and it is the purpose of this paper to bring together the available information pertaining to the improvement of alfalfa and to add certain suggestions with respect to future …


Nebraska Pastures: Seeding And Management, A. L. Frolik, E. F. Frolik Feb 1941

Nebraska Pastures: Seeding And Management, A. L. Frolik, E. F. Frolik

Nebraska Agricultural Experiment Station: Historical Circulars

Pastures are an important source of feed in the livestock industry. Approximately 70 per cent of the income from Nebraska farms is from the sale of livestock and livestock products, and for this reason consideration should be given to the maintenance of good pastures. Poor pastures, like other poor crops, are expensive. Good grass cover is also an effective means of conserving the soil resources.


Fifty Years Of Achievement In Agricultural Investigation, R. T. Prescott Mar 1939

Fifty Years Of Achievement In Agricultural Investigation, R. T. Prescott

Nebraska Agricultural Experiment Station: Historical Circulars

In Nebraska, a hustling frontier state in 1887, the legislature hesitated not at all in taking advantage of the provisions of the Hatch Act, and now that fifty years have elapsed since the Station was founded, seventy-five years since the Land Grant College Act was passed and the U. S. Department of Agriculture established, and almost twenty-five years since the Agricultural Extension Service was added, it seems worth while to present a general summary of achievement within the state. The main object will be to show some of the important things that have been learned through the investigations of the …


Wilt And Cold Resistance Of Self-Fertilized Lines Of Alfalfas, George L. Peltier, H. M. Tysdal Sep 1934

Wilt And Cold Resistance Of Self-Fertilized Lines Of Alfalfas, George L. Peltier, H. M. Tysdal

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

The relative reactions to cold and wilt of self-fertilized lines of alfalfa through to the fifth generation are reported. Some of the reasons for undertaking a breeding program are given as well as methods used in the controlled cold and wilt determinations. Preliminary studies showed that reinoculating healthy individuals which had already resisted one wilt infection test served to eliminate additional plants, but the percentage healthy was much higher after the reinoculation than after the first inoculation. The foundation materials from which selections were made consisted of selected plants from a number of old Nebraska fields and a large collection …


The Nature Of Resistance In Alfalfa To Wilt (Aplanobacter Insidiosum L. Mc.), George L. Peltier, F. R. Schroeder Oct 1932

The Nature Of Resistance In Alfalfa To Wilt (Aplanobacter Insidiosum L. Mc.), George L. Peltier, F. R. Schroeder

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

Alfalfa wilt was first discovered in 1924 in southern Wisconsin and northern Illinois. The causal organism, Aplanobacter insidiosum, was later described by McCulloch. Since that time wilt has been reported from most of the alfalfa-growing sections of the United States and in some states it has been responsible for a rapid decrease in the acreage of alfalfa. During the past three years a number of investigators have found that certain alfalfas are somewhat resistant to wilt, whereas others are very susceptible. At the Nebraska station the results to date indicate that all common and most variegated alfalfas are very …


The Relative Susceptibility Of Alfalfas To Wilt And Cold, George L. Peltier, H. M. Tysdal Dec 1930

The Relative Susceptibility Of Alfalfas To Wilt And Cold, George L. Peltier, H. M. Tysdal

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

The final solution of the alfalfa-wilt problem in Nebraska probably lies in the development of resistant sorts that are winterhardy as well as productive. The relative susceptibility to wilt of 40 lots from Turkestan, 10 from France, 6 of Grimm from as many states, and 16 domestic alfalfas are reported on at this time, together with hardiness tests of a few well-known varieties and strains and the more recent introductions from Turkestan and France.


Alfalfa Investigations, T. A. Kiesselbach, Arthur Anderson Dec 1926

Alfalfa Investigations, T. A. Kiesselbach, Arthur Anderson

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

The purpose of these alfalfa investigations has been to determine the relation of variety, source of seed, and cultural practice to the yield and quality of hay produced. Due to the high initial cost of seeding, the inconvenience and wastefulness of losing a stand prematurely, and the superiority of properly-made hay, an understanding of these relationships is very important.


Hardy Alfalfa For Nebraska, T. A. Kiesselbach, A. Anderson Aug 1925

Hardy Alfalfa For Nebraska, T. A. Kiesselbach, A. Anderson

Nebraska Agricultural Experiment Station: Historical Circulars

Comparative tests of varieties and regional strains of common alfalfa at the Nebraska Experiment Station have clearly indicated that winter hardiness is an important factor to be considered when purchasing alfalfa seed. Much of the seed sold in the United States has proved inferior in this regard for Nebraska conditions. The use of southern-grown domestic seed or seed imported from countries with a much milder climate than that of Nebraska should be avoided in this state. On the other hand, reliance may be placed on the seed of hardy varieties or hardy regional strains of common alfalfa grown in Nebraska …