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Full-Text Articles in Life Sciences

18s-Nemabase: Curated 18s Rrna Database Of Nematode Sequences, Kaitlin Gattoni, Eli M. S. Gendron, Rebeca Sandoval- Ruiz, Abigail Borgemeier, J. Parr Mcqueen, Rachel M. Shepherd, Dieter Slos, Tom Powers, Dorota L. Porazinska Aug 2022

18s-Nemabase: Curated 18s Rrna Database Of Nematode Sequences, Kaitlin Gattoni, Eli M. S. Gendron, Rebeca Sandoval- Ruiz, Abigail Borgemeier, J. Parr Mcqueen, Rachel M. Shepherd, Dieter Slos, Tom Powers, Dorota L. Porazinska

Department of Plant Pathology: Faculty Publications

Nematodes are the most abundant and diverse animals on the planet but lack representation in biodiversity research. This presents a problem for studying nematode diversity, particularly when molecular tools (i.e., barcoding and metabarcoding) rely on well-populated and curated reference databases, which are absent for nematodes. To improve molecular identification and the assessment of nematode diversity, we created and curated an 18S rRNA database specific to nematodes (18S-NemaBase) using sequences sourced from the most recent publicly available 18S rRNA SILVA v138 database. As part of the curation process, taxonomic strings were standardized to contain a fixed number of taxonomic ranks relevant …


18s-Nemabase: Curated 18s Rrna Database Of Nematode Sequences, Kaitlin Gattoni, Eli M. S. Gendron, Rebeca Sandoval- Ruiz, Abigail Borgemeier, J. Parr Mcqueen, Rachel M. Shepherd, Dieter Slos, Tom Powers, Dorota L. Porazinska Aug 2022

18s-Nemabase: Curated 18s Rrna Database Of Nematode Sequences, Kaitlin Gattoni, Eli M. S. Gendron, Rebeca Sandoval- Ruiz, Abigail Borgemeier, J. Parr Mcqueen, Rachel M. Shepherd, Dieter Slos, Tom Powers, Dorota L. Porazinska

Department of Plant Pathology: Faculty Publications

Nematodes are the most abundant and diverse animals on the planet but lack representation in biodiversity research. This presents a problem for studying nematode diversity, particularly when molecular tools (i.e., barcoding and metabarcoding) rely on well-populated and curated reference databases, which are absent for nematodes. To improve molecular identification and the assessment of nematode diversity, we created and curated an 18S rRNA database specific to nematodes (18S-NemaBase) using sequences sourced from the most recent publicly available 18S rRNA SILVA v138 database. As part of the curation process, taxonomic strings were standardized to contain a fixed number of taxonomic ranks relevant …


Western Prairie Fringed Orchid Management, Ecology, And Decline At Mormon Island, Andrew J. Caven Mar 2022

Western Prairie Fringed Orchid Management, Ecology, And Decline At Mormon Island, Andrew J. Caven

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The western prairie fringed orchid (Platanthera praeclara Sheviak & M. L. Bowles; WPFO) was first detected in a vegetative state on Mormon Island in 1978 and identification was confirmed following a mass flowering event in 1982. From a high count of ~60 plants the WPFO slowly declined and has not been observed since 2000 despite flowering season surveys conducted in 15 of the last 20 years. We explore the natural history of the WPFO in the contexts of Mormon Island to establish potential causes for its apparent disappearance and evaluate the possibility it persists in some capacity. Our investigation …


Gaia Contributions To Agroecology By James Lovelock (1919-2022), Steve Gliessman, Charles A. Francis Jan 2022

Gaia Contributions To Agroecology By James Lovelock (1919-2022), Steve Gliessman, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

In writing about the history of agroecology we too often ignore the valuable contributions of British scientist James Lovelock who recently died on his 103rd birthday. A prolific inventor and influential theorist, Lovelock is best known for the Gaia hypothesis first proposed during his innovative work in the 1960s with the United States National Aeronautics and Space Administration. He suggested that ‘the biosphere has a regulatory effect on the Earth’s environment that acts to sustain life’ as written in Gaia: A New Look at Life on Earth (Lovelock 1979). Lovelock further proposed that humans have strongly impacted the planet’s capacity …