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Bacteriocin-Like Substances Produced By Rhizobium Japonicum And Other Slow-Growing Rhizobia, D. C. Gross, A. K. Vidaver
Bacteriocin-Like Substances Produced By Rhizobium Japonicum And Other Slow-Growing Rhizobia, D. C. Gross, A. K. Vidaver
Department of Plant Pathology: Faculty Publications
Bacteriocin-like substances were commonly produced by slow-growing Rhizobium japonicum and cowpea rhizobia on an L-arabinose medium. Antagonism between strains of R. japonicum was not detected in vitro; however, such strains were often sensitive to some bacteriocins produced by cowpea rhizobia. Inhibitory zones (2 to 8 mm from colony margins), produced by 58 of 66 R. japonicum test strains, were reproducibly detected with Corynebacterium nebraskense as an indicator. Quantitative production was not related to symbiotic properties of effective strains, since nine noninfective strains and one ineffective strain produced bacteriocin. Eight R. japonicum strains that did not produce bacteriocin nevertheless formed effective …
Simple Procedure For Disruption Of Fungal Spores, James L. Van Etten, Shelby Freer
Simple Procedure For Disruption Of Fungal Spores, James L. Van Etten, Shelby Freer
Department of Plant Pathology: Faculty Publications
A simple, inexpensive method for disrupting dormant fungal spores is described.
Purification Of Some Legume Carlaviruses, Venkateswarlu Veerisetty, Myron K. Brakke
Purification Of Some Legume Carlaviruses, Venkateswarlu Veerisetty, Myron K. Brakke
Department of Plant Pathology: Faculty Publications
Purification schemes were developed which yielded 0.7 to 1.0 mg of alfalfa latent virus (ALV) and pea streak virus (PSV) and 0.1 to 0.3 mg of red clover vein mosaic virus (RCVMV) per gram pea cullivar Lincoln plant tissue (excluding roots). The freezing of the tissue and the use of an appropriate extraction buffer were crucial. Virus from sap was precipitated by 6%(w/v) polyethylene glycol (PEG, MW 6,000) and concentrated by two cycles of differential centrifugation. Partially purified virus preparations had a single nucleoprotein component in rate-zonal sucrose and equilibrium cesium chloride density gradient centrifugation. The virus preparations did not …
Nitrogen Fixation (Acetylene Reduction) Associated With Roots Of Winter Wheat And Sorghum In Nebraska, Wayne L. Pedersen, K. Chakrabarty, R. V. Klucas, Anne K. Vidaver
Nitrogen Fixation (Acetylene Reduction) Associated With Roots Of Winter Wheat And Sorghum In Nebraska, Wayne L. Pedersen, K. Chakrabarty, R. V. Klucas, Anne K. Vidaver
Department of Plant Pathology: Faculty Publications
Root segments and root-soil cores (6.5-cm diameter) from fields and nurseries of winter wheat and sorghum were tested for N2 fixation by using the acetylene reduction assay. Wheat samples (~1,200) from 109 sites generally had low or no activity (0 to 3.1 nmol of C2H4 produced per h per g [dry weight] of root segments), even after 24 h of incubation. However, a commercial field of Scout 66, located in western Nebraska, exhibited appreciable activity (290 nmol of C2H4 produced per h per g [dry weight] of root segments). Of 400 sorghum lines …
Purification Of Some Legume Carlaviruses, Venkateswarlu Veerisetty, Myron K. Brakke
Purification Of Some Legume Carlaviruses, Venkateswarlu Veerisetty, Myron K. Brakke
Department of Plant Pathology: Faculty Publications
Purification schemes were developed which yielded 0.7 to 1.0 mg of alfalfa latent virus (ALV) and pea streak virus (PSV) and 0.1 to 0.3 mg of red clover vein mosaic virus (RCVMV) per gram pea cullivar Lincoln ) plant tissue (excluding roots). The freezing of the tissue and the use of an appropriate extraction buffer were crucial. Virus from sap was precipitated by 6%(w/v) polyethylene glycol (PEG, MW 6,000) and concentrated by two cycles of differential centrifugation. Partially purified virus preparations had a single nucleoprotein component in rate-zonal sucrose and equilibrium cesium chloride density gradient centrifugation. The virus preparations did …