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Plant Sciences

University of Kentucky

Molecular biology

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Full-Text Articles in Life Sciences

Opportunities In Molecular Biology: Enhancement Of The Nutritional Value Of Forages, K. R. Hancock, M. J. Ulyatt Nov 2021

Opportunities In Molecular Biology: Enhancement Of The Nutritional Value Of Forages, K. R. Hancock, M. J. Ulyatt

IGC Proceedings (1997-2023)

Pasture plants, while producing a cost-effective source of feed for grazing ruminants, can frequently be less than optimal in meeting the animals’ nutrient requirements. Over the past half century, there has been a major effort to improve the quantity and nutritional quality of pasture plants using conventional plant breeding. Although considerable progress has been made in improving the quality and agronomic characteristics of our major pasture plants by this means, breeding can only be applied to plants capable of sexual crosses. This poses severe limitations both in terms of speed of progress and in the number of genes available for …


Reference Gene Selection For Transcriptional Profiling In Cryptocercus Punctulatus, An Evolutionary Link Between Isoptera And Blattodea, Zhen Li, Xiangrui Li, Qingwen Zhang, Ling Yuan, Xuguo Zhou Dec 2020

Reference Gene Selection For Transcriptional Profiling In Cryptocercus Punctulatus, An Evolutionary Link Between Isoptera And Blattodea, Zhen Li, Xiangrui Li, Qingwen Zhang, Ling Yuan, Xuguo Zhou

Plant and Soil Sciences Faculty Publications

The subsocial life style and wood-feeding capability of Cryptocercus gives us an evolutionary key to unlock some outstanding questions in biology. With the advent of the Genomics Era, there is an unprecedented opportunity to address the evolution of eusociality and the acquisition of lignocellulases at the genetic level. However, to quantify gene expression, an appropriate normalization strategy is warranted to control for the non-specific variations among samples across different experimental conditions. To search for the internal references, 10 housekeeping genes from a gut transcriptome of a wood-feeding cockroach, Cryptocercus punctulatus, were selected as the candidates for the RT-qPCR analysis. The …