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Development Of Near-Isogenic Lines In A Parthenogenetically Reproduced Thrips Species, Frankliniella Occidentalis, Guangdi Yuan, Yanran Wan, Xiaoyu Li, Bingqing He, Youjun Zhang, Baoyun Xu, Shaoli Wang, Wen Xie, Xuguo Zhou, Qingjun Wu

Entomology Faculty Publications

Although near-isogenic lines (NILs) can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. Here, through multiple rounds of backcrossing and spinosad screening, we generated spinosad resistant NILs in the western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), with a haplo-diploid reproduction system. The resultant F. occidentalis NIL-R strain maintained a resistance ratio over 30,000-fold, which was comparable to its parental resistant strain, Spin-R. More importantly, F. occidentalis NIL-R shared 98.90% genetic similarity with its susceptible parental strain Ivf03. By developing this toolset, we are able to segregate individual resistance and facilitate the mechanistic study …