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Full-Text Articles in Life Sciences
Using Single Cell Type Proteomics To Identify Al-Induced Proteomes In Outer Layer Cells And Interior Tissues In The Apical Meristem/Cell Division Regions Of Tomato Root-Tips, Jesse Potts, Hui Li, Yaoguo Qin, Xingbo Wu, Dafeng Hui, Kamal Al Nasr, Suping Zhou, Yang Yong, Tara Fish, Jiping Liu, Theodore W. Thannhauser
Using Single Cell Type Proteomics To Identify Al-Induced Proteomes In Outer Layer Cells And Interior Tissues In The Apical Meristem/Cell Division Regions Of Tomato Root-Tips, Jesse Potts, Hui Li, Yaoguo Qin, Xingbo Wu, Dafeng Hui, Kamal Al Nasr, Suping Zhou, Yang Yong, Tara Fish, Jiping Liu, Theodore W. Thannhauser
Biology Faculty Research
Aluminum (Al) toxicity primarily targets the root tips, inhibiting root growth and function and leading to crop yield losses on acidic soils. Previously we reported using laser capture microdissection (LCM) proteomics to identify Al-induced proteins in the outer layer cells in the transitional zone of tomato root-tips. This study aims to further characterize Al-induced proteomic dynamics from the outer to interior tissues, thus providing a panoramic view reflecting Al resistance in the root tip as a whole in tomatoes. Three types of cells were isolated via LCM from the basal 350–400 μm (below cell elongation regions) of root tips using …