Life Sciences Commons^™

The Al-Induced Proteomes Of Epidermal And Outer Cortical Cells In Root Apex Of Cherry Tomato 'La 2710', Shaolan Yang, Hui Li, Sarabjit Bhatti, Suping Zhou, Yong Yang, Tara Fish, Theodore W. Thannhauser

Agricultural and Environmental Sciences Faculty Research

This paper reports a laser capture microdissection-tandem mass tag-quantitative proteomics analysis of Al-sensitive cells in root tips. Cherry tomato (Solanum lycopersicum var. cerasiforme ‘LA2710’) seedlings were treated under 15 μM Al³⁺ activity for 13 d. Root-tip longitudinal fresh frozen tissue sections of 10 μm thickness were prepared. The Al-sensitive root zone and cells were determined using histochemical analysis of root-tips and micro-sections. A procedure for collecting the Al-sensitive cells using laser capture microdissection-protein extraction-tandem mass tag-proteomics analysis was developed. Proteomics analysis of 18 μg protein/sample with three biological replicates per treatment condition identified 3879 quantifiable proteins each associated …