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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Plant Sciences

South Dakota State University

Theses/Dissertations

2017

CRISPR/Cas9

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Silencing Seed Dormancy Genes To Mitigate Risk Of Transgene Flow To Weedy Rice, Alexander Wireko Kena Jan 2017

Silencing Seed Dormancy Genes To Mitigate Risk Of Transgene Flow To Weedy Rice, Alexander Wireko Kena

Electronic Theses and Dissertations

The flow of fitness-enhancing transgenes from genetically modified crops into wild/weed relatives may cause serious ecological and economic consequences. Seed dormancy (SD) is a key adaptive trait that distributes germination over time, resulting in weed persistence in agroecosystems. Thus, silencing major genes controlling SD would reduce the adaptive fitness of weeds. SD-enhancing genes cloned from weedy rice include SD7-1, SD7-2, SD12a, SD12b, and SD12c. The goal of this study was to develop a transgenic mitigation (TM) strategy using SD gene-silencing structures as mitigating factors to reduce the risk of transgene flow to wild/weed populations. TM vector constructs consisted of the …