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Plant Sciences

Dartmouth College

2013

Polymerase chain reaction

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Full-Text Articles in Life Sciences

Dirigent Domain-Containing Protein Is Part Of The Machinery Required For Formation Of The Lignin-Based Casparian Strip In The Root, Prashant S. Hosmani, Takehiro Kamiya, John Danku, Sadaf Naseer, Niko Geldner, Mary Lou Guerinot, David Salt Aug 2013

Dirigent Domain-Containing Protein Is Part Of The Machinery Required For Formation Of The Lignin-Based Casparian Strip In The Root, Prashant S. Hosmani, Takehiro Kamiya, John Danku, Sadaf Naseer, Niko Geldner, Mary Lou Guerinot, David Salt

Dartmouth Scholarship

The endodermis acts as a "second skin" in plant roots by providing the cellular control necessary for the selective entry of water and solutes into the vascular system. To enable such control, Casparian strips span the cell wall of adjacent endodermal cells to form a tight junction that blocks extracellular diffusion across the endodermis. This junction is composed of lignin that is polymerized by oxidative coupling of monolignols through the action of a NADPH oxidase and peroxidases. Casparian strip domain proteins (CASPs) correctly position this biosynthetic machinery by forming a protein scaffold in the plasma membrane at the site where …


Identification Of Cytokinin-Responsive Genes Using Microarray Meta-Analysis And Rna-Seq In Arabidopsis, Apurva Bhargava, Ivory Clabaugh, Jenn P. To, Bridey B. Maxwell, Yi-Hsuan Chiang, G. Eric Schaller, Ann Loraine, Joseph J. Kieber May 2013

Identification Of Cytokinin-Responsive Genes Using Microarray Meta-Analysis And Rna-Seq In Arabidopsis, Apurva Bhargava, Ivory Clabaugh, Jenn P. To, Bridey B. Maxwell, Yi-Hsuan Chiang, G. Eric Schaller, Ann Loraine, Joseph J. Kieber

Dartmouth Scholarship

Cytokinins are N6-substituted adenine derivatives that play diverse roles in plant growth and development. We sought to define a robust set of genes regulated by cytokinin as well as to query the response of genes not represented on microarrays. To this end, we performed a meta-analysis of microarray data from a variety of cytokinin-treated samples and used RNA-seq to examine cytokinin-regulated gene expression in Arabidopsis (Arabidopsis thaliana). Microarray meta-analysis using 13 microarray experiments combined with empirically defined filtering criteria identified a set of 226 genes differentially regulated by cytokinin, a subset of which has previously been …