Life Sciences Commons^™

In Vivo Dose-Response Of Insects To Hz-2v Infection, John P. Burand, Christopher P. Rallis

John Burand

Background Hz-2V infection of female Helicoverpa zea moths is manifested as insects that are either sterile "agonadal" individuals with malformed reproductive tissues or fertile asymptomatic carriers which are capable of transmitting virus on to their progeny. Virus infected progeny arising from eggs laid by asymptomatic carrier females may themselves be either sterile agonadals or asymptomatic carriers. Results By injecting virus into female moths, a correlation was established between virus doses administered to the females and the levels of resulting asymptomatic and sterile progeny. Conclusions The results of these experiments indicate that high virus doses produced a higher level of agonadal …

West River Ag Center Crops And Soils Research Annual Progress Report, 2004, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2004 progress report of the West River Crops and Soils Research Projects, South Dakota Agricultural Experiment Station. This document includes reports on: weather and climate, wheat and grain variety trials, management and tillage, and weed and pest control.

Expression Of The Tobacco Β- 1,3-Glucanase Gene, Pr-2d, Following Induction Of Sar With Peronospora Tabacina, Deanna L. Funnell, Christopher B. Lawrence, Jeffrey F. Pedersen, Christopher L. Schardl

Department of Plant Pathology: Faculty Publications

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic P-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to …

Distance Between Pollen Donor And Recipient Influences Fruiting Success In Slickspot Peppergrass, Lepidium Papilliferum, Ian C. Robertson, Amy C. Ulappa

Biology Faculty Publications and Presentations

Plant populations are often spatially structured owing to limited dispersal of pollen and seed. Mating between neighboring individuals in such populations often leads to reduced reproductive performance relative to matings between distant individuals. This response, which may be a result of inbreeding depression or prezygotic mating barriers, was investigated for slickspot peppergrass, Lepidium papilliferum L. (Brassicaceae), a rare insect-pollinated mustard endemic to sagebrush–steppe habitat in southwestern Idaho. Through hand pollination experiments we found that individual plants receiving pollen from distant sources (75–100 m and 6.5–20 km away) had significantly higher percent fruit sets than those relying on pollen from neighboring …

Atrazine Runoff In The Blue River Basin: Geomorphology, Rainfall, And Agronomic Practices, Kundan Dhakal

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Atrazine concentrations in the Big Blue River Basin (BRB) in Nebraska and Kansas periodically exceed the U.S. EPA Maximum Contaminant Level (MCL) of 3 µg L^-1. The present study is focused on watershed variables influencing surface runoff of atrazine. The assessment includes the impact of corn and sorghum planting progress (indicating atrazine application), rainfall, antecedent soil water content, and soil restrictive layer on stream-measured weekly atrazine load in independent BRB subwatersheds for 1997 - 2004. Maximum atrazine loading occurred after most of the corn had been planted but during sorghum planting from mid-May to early June, immediately following …

In Vitro Propagation, Regeneration, Attempted Tetraploid Induction, And Agrobacterium-Mediated Transformation Of Euphorbia Pulchurrima ‘Winter Rose’™, Kimberly Ann Pickens

Doctoral Dissertations

Poinsettia, Euphorbia pulchurrima, is the number one potted flowering plant in the United States. ‘Winter Rose’™ is a very popular cultivar with more than one million plants sold each year. To further improve this cultivar, particularly for larger flower heads and free branching, this research aimed at establishing some in vitro systems for application of biotechnology to poinsettia genetic improvement.

A protocol was established for in vitro axillary bud proliferation using greenhouse grown terminal buds. Buds were placed on Murashige-Skoog (MS) basal medium supplemented with benzlyaminopurine (BA). Explants produced the greatest number of axillary buds on medium containing between 2.2-8.8 …

Arkansas Soybean Performance Tests 2004, D. G. Dombek, D. K. Ahrent, R. D. Bond, I. L. Eldridge

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Pineywoods Native Plant Center News, December 2004, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Newsletters

No abstract provided.

Mutations In Pmr5 Result In Powdery Mildew Resistance And Altered Cell Wall Composition, John P. Vogel, Ted K. Raab, Chris R. Somerville, Shauna C. Somerville

Ted K. Raab

Powdery mildews and other obligate biotrophic pathogens are highly adapted to their hosts and often show limited host ranges. One facet of such host specialization is likely to be penetration of the host cell wall, a major barrier to infection. A mutation in the pmr5 gene rendered Arabidopsis resistant to the powdery mildew species Erysiphe cichoracearum and Erysiphe orontii, but not to the unrelated pathogens Pseudomonas syringae or Peronospora parasitica. PMR5 belongs to a large family of plant-specific genes of unknown function. pmr5-mediated resistance did not require signaling through either the salicylic acid or jasmonic acid/ ethylene defense pathways, suggesting …

Center For Grassland Studies Newsletter, Winter 2004, Volume 10, No. 1

Center for Grassland Studies: Newsletters

Matching Beef Cattle Genetics to Feed Resources by Jim Gosey, Department of Animal Science, UNL

Ecological Monitoring to Assist Producers with Grassland Production Forecasts by George Green, School of Natural Resources, UNL

Nebraska Ranch Practicum Enters Sixth Year by Jerry Volesky, Don Adams, Richard Clark, Dillon Feuz, Patrick Reece, Brent Plugge, Bud Stolzenburg and Troy Walz, UNL

Bull Riders for Biodiversity? – And Other Heresies for the Northern Great Plains by Tyler Sutton, for The Conservation Alliance of the Great Plains

2004 Nebraska Grazing Conference to be Aug. 10-11

The Nebraska Turfgrass Conference: 42 Years of Educating Nebraska Turfgrass Managers …

Agronomic Responses Of Corn Hybrids From Different Eras To Deficit And Adequate Levels Of Water And Nitrogen, Patrick M. O'Neill, J.F. Shanahan, James S. Schepers, Bob Caldwell

Department of Agronomy and Horticulture: Faculty Publications

Maintaining current high yields of corn (Zea mays L.) grown in the USA poses an environmental threat due to continued overuse of water and N inputs. To reduce overreliance on inputs, future corn breeding efforts should focus on improving tolerance of corn to water and N stresses, utilizing appropriate tolerance mechanisms. The objective of this study was to identify appropriate mechanisms by characterizing agronomic responses of 12 hybrids from three different eras (‘B73 x Mo17’ from 1970s and three early 1990s and eight late 1990s Pioneer brand hybrids) to varying water and N supply. This was done by growing …

Southeast South Dakota Experiment Farm Annual Progress Report, 2004, Agricultural Experiment Station

Agricultural Experiment Station and Research Farm Annual Reports

This report contains 30 crop and livestock research and demonstration summaries of projects conducted at Southeast Research Farm in 2004. This year’s cattle report describes a project that compares feeding dried distillers grains to other oilseed supplements for wintering cows on ground corn stalks. Our crop reports show results of the many weed control projects that were conducted here as well as variety trial results and breeder evaluations for oat, corn, soybean, and forages. Several soil fertility research projects focused on strip/zone till, amending soils with gypsum, nutrient management associated with livestock manure, fertilizer placement, and other topics. Insects, soybean …

Land Rehabilitation And Maintenance Equipment Sources, Heidi Howard, Shannon Mcdowell, Amanda Breon, John A. Guretzky, Bradley Tadlock

Department of Agronomy and Horticulture: Faculty Publications

Military training can often be destructive to the environment. Environmental impacts associated with heavy training include soil compaction, soil erosion, siltation of waterways and wetlands, increased threat of flooding, loss of wildlife habitat, declining biodiversity, and invasion by noxious weeds. Planning and implementing land rehabilitation projects generally involves some soil movement, establishment of vegetation, stabilization and hardening of sites, etc. Procurement of appropriate heavy equipment and other specialized equipment is sometimes a difficult task that involves obtaining multiple vendors, especially when procuring equipment for rental, lease, or purchase. This report and associated database were created to help land managers easily …

Sources Of Plant Materials For Land Rehabilitation, Heidi Howard, John A. Guretzky, Brandy Koch, Shannon Mcdowell, Steven Warren, Gwyn L. Howard

Department of Agronomy and Horticulture: Faculty Publications

Military training often results in environmental degradation that has negative impacts on future training missions. The U.S. Army Construction Engineering Research Laboratory (CERL) conceptualized the Army’s Integrated Training Area Management (ITAM) program in response to the concerns expressed by military land managers and trainers over the declining condition of training and testing lands. ITAM has evolved to include land condition and trend analysis (LCTA), environmental awareness (EA), training requirements integration (TRI), and land rehabilitation and maintenance (LRAM). The need for LRAM has increased dramatically over the past decade. Among the many difficulties faced in planning and implementing a land rehabilitation …

Forage News [2004-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forages at KCA in Owensboro
• Kentucky Alfalfa Conference – 25^TH Anniversary
• Groups Form National Alfalfa Coalition
• Eleven Seasons of Pasture Productivity and Heifer Performance Under Rotational Grazing: Data From WICST.
• Hay Storage Update
• Upcoming Events

Arkansas Corn And Grain Sorghum Performance Tests 2004, D. G. Dombek, D. K. Ahrent, R. D. Bond, I. L. Eldridge

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

The Prairie Naturalist Volume 36, No.4 December 2004

The Prairie Naturalist

ROADSIDE BIAS IN POINT COUNT SURVEYS AT ARROWWOOD NATIONAL WILDLIFE REFUGE, NORTH DAKOTA ▪ J. S. Dieni and P. Scherr

HISTORY OF GREATER SAGE-GROUSE IN THE DAKOTAS: DISTRIBUTION AND POPULATION TRENDS ▪ . J. T. Smith, L. D. Flake, K. F. Higgins, and G. D. Kobriger

PATCH USE BY A MONOPHAGOUS HERBIVORE IN FRAGMENTED PRAIRIE LANDSCAPES ▪ . M. J. St. Pierre and S. D. Hendrix

SNOWSHOE HARE OCCURRENCE AND WINTER HABITAT ON THE MISSOURI COTEAU IN NORTH DAKOTA ▪ R. K. Murphy 243

HIGH ABUNDANCE OF NESTING LONG-EARED OWLS IN NORTH DAKOTA ▪ R. K. Murphy, L. J. Rosenfield, …

Levels And Patterns Of Genetic Diversity In The Rare And Endangered Cumberland Stitchwort, Minuartia Cumberlandensis (Caryophyllaceae), Charles Thomas Winder

Masters Theses

Sequences of a highly variable nuclear gene (G3pdh) were used to characterize genetic diversity within and among populations of the endangered rockhouse endemic, Minuartia cumberlandensis (Wofford and Kral) McNeill (Caryophyllaceae), and compared to a widespread and abundant related species, M. glabra (Michaux) McNeill. By reconstructing phylogenetic relationships among G3pdh variants (haplotypes) and observing the geographical distribution of those ordered variants, an attempt was made to gauge the effects of historical and contemporary population processes acting within the species, particularly those with potential implications for long-term conservation. Both M. cumberlandensis and M. glabra were found to have high overall …

Evaluation Of Botanical Oil Formulations For Management Of Powdery Mildew And Mites, Adriane Lorraine Cannon

Masters Theses

Experiments were conducted to evaluate the effects of newly created soybean oil formulations on powdery mildew (Erysiphe pulphra ), photosynthesis, phytotoxicity, and broad mites (Polyphagotarsonemus latus (Banks)) on dogwood ( Cornus florida (L.) 'Cloud Nine') trees, and to evaluate the amounts of formulations that are deposited and washed off of peach (Prunus persica, (L.)) leaves, dormant oak (Quercus phellos, (L.) ), and viburnum ( Viburnum x juddii) twigs after simulated rainfall. Oil treatments were applied using a back pack mist blower sprayed pre- and post- inoculation of powdery mildew to evaluate powdery mildew, …

Seasonal Abundance Of Mosquitoes (Diptera: Culicidae) At A High And Low Prevalence Site For La Crosse Encephalitis In Eastern Tennessee, Nathan David Caldwell

Masters Theses

La Crosse (LAC), a California (CAL) serogroup bunyavirus, is the most prevalent pediatric arboviral disease in the United States and accounts for virtually all encephalitis cases associated with CAL serogroup arboviruses. In Tennessee, USA, prior to the drastic increase of confirmed La Crosse (LAC) encephalitis cases in 1997, the sum of documented cases reported to the Tennessee Department of Health totaled 9. During subsequent years in Tennessee (1997 to 2003), an average of 12 cases has been reported annually. Approximately 62% of these reported cases have occurred in the months of July and August. The native, container-inhabiting mosquito Ochlerotatus triseriatus …

Compost As A Growth Substrate For Woody Ornamental Nursery Production, Jaime Crocker

Masters Theses & Specialist Projects

Peat is an expensive, environmentally nonrenewable component of media used to grow woody ornamental trees. Leaf compost from the city of Bowling Green is an inexpensive renewable resource that was substituted for peat in soil-less container media. Seeds of three commercially important tree species, Koelreuteria paniculata (goldenraintree), Quercus alba (white oak), and Magnolia grandiflora (southern magnolia) were grown in 473 mL bottomless milk containers for 150 days, then repotted to 3785 mL pots for 90 days. Media ratios were 1:0, 3:1, 1:1, 1:3, and 0:1 parts commercial media to leaf compost from the Western Kentucky University Agricultural Research Center in …

Phoradendron Leucarpum (Raf.) Reveal & M.C. Johnston, Loy R. Phillippe

Specimens by Name

No abstract provided.

Collaborative Research: Developing Methods To Study Age-Related Changes In The Physiology Of Forest Trees, Michael S. Greenwood

University of Maine Office of Research Administration: Grant Reports

Trees continue to increase in size and complexity over life spans that may last many centuries, and each year the growing regions of their shoots produce new foliage and reproductive structures. As they grow older and larger, forest trees appear to grow more slowly, produce thicker foliage and more reproductive structures. These changes are likely to be associated with age-related declines in the growth rates of forest stands, which occur before the mid-point of the lifespan of important timber species. Although similar patterns of age- and size-related change in the morphology and physiology of foliage have been described for numerous …

C-Terminal 23 Kda Polypeptide Of Soybean Gly M Bd 28 K Is A Potential Allergen, Ping Xiang, Eric J. Haas, Michael G. Zeece, John P. Markwell, Gautam Sarath

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Gly m Bd 28 K is a major soybean (Glycine max Merr.) glycoprotein allergen. It was originally identified as a 28 kDa polypeptide in soybean seed flour. However, the full-length protein is encoded by an open reading frame (ORF) of 473 amino acids, and contains a 23 kDa C-terminal polypeptide of as yet unknown allergenic and structural characteristics. IgE-binding (allergenic potential) of the Gly m Bd 28 K protein including the 23 kDa C-terminal portion as well as shorter fragments derived from the full-length ORF were evaluated using sera from soy-sensitive adults. All of these sera contained IgE that …

Nebraska Vine Lines, Vol. Viii, No. 6. November/December 2004

Viticulture Publications and Research

• Fall Workshop: Instrumentation for Vineyards and Wineries – 95 attend.

• Edward Hellman

• The Grand Banquet to feature “Ragtime”

• Alcohol and Tobacco Tax and Trade Bureau to Speak at the March Forum in Kearney, NE

• Last Chance Winery

• Pioneer Nebraska Winery Turns “10”

• Taste Buds

• Masters Students to speak at 7th Forum in Kearney

Shiga Toxin Binding To Isolated Porcine Tissues And Peripheral Blood Leukocytes, Kellie R. K. Winter, William C. Stoffregen, Evelyn A. Dean-Nystrom

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Shiga toxin (Stx) binding sites in porcine tissues and leukocytes were identified by the use of Stx overlay and anti-CD77/Gb3 immunoassays. Stx1 and Stx2 bound to similar tissue locations and leukocytes, although some differences were noted. Previously unreported Stx binding sites were identified in kidney tubules, intestinal lymphoid aggregates, sinusoidal liver cells, alveolar macrophages, and peripheral blood leukocytes.

Forage News [2004-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Dr. Ray Smith Joins Kentucky Forage Team
• KFGC Presents 2004 Forage Awards
• Forage Spokesman Winner
• Grigson Becomes KFGC President
• KFGC Board Elects Six New Members
• 5th Kentucky Grazing Conference Big Success
• Forage Information
• What Is the Quality of Your Hay?
• How Good Is Stockpiled Tall Fescue?
• Effects of Tall Fescue Endophyte on Reproduction
• American Forage and Grassland Council Update
• Calf Weaning Weights of Cattle Grazing Toxic or MaxQ™ Tall Fescue Stands
• Hay Production
• Upcoming Events

Pineywoods Native Plant Center, Dec 2004, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Newsletters

No abstract provided.

Irrigation System Maintenance, Kelly Kopp, Jennie Hoover

CWEL Extension Fact Sheets

No abstract provided.

Tandem Inverted Repeat System For Selection Of Effective Transgenic Rnai Strains In Chlamydomonas, Jennifer Rohr, Nandita Sarkar, Susan Belanger, Byeong-Ryool Jeong, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty and Staff Publications

RNA interference (RNAi), the double-stranded RNA (dsRNA) triggered post-transcriptional gene silencing, is becoming a powerful tool for reverse genetics studies. Stable RNAi, induced by the expression of inverted repeat (IR) transgenes, has been achieved in protozoa, algae, fungi, plants, and metazoans. However, the level of gene silencing is often quite variable, depending on the type of construct, transgene copy number, site of integration, and target gene. This is a hindrance in functional genomics studies, where it is desirable to suppress target genes reliably to analyze unknown phenotypes. Consequently, we explored strategies for direct selection of effective transgenic RNAi lines in …