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Suberin Biosynthesis And Deposition In The Wound-Healing Potato (Solanum Tuberosum L.) Tuber Model, Kathlyn Natalie Woolfson
Suberin Biosynthesis And Deposition In The Wound-Healing Potato (Solanum Tuberosum L.) Tuber Model, Kathlyn Natalie Woolfson
Electronic Thesis and Dissertation Repository
Suberin is a heteropolymer comprising a cell wall-bound poly(phenolic) domain (SPPD) covalently linked to a poly(aliphatic) domain (SPAD) that is deposited between the cell wall and plasma membrane. Potato tuber skin contains suberin to protect against water loss and microbial infection. Wounding triggers suberin biosynthesis in usually non-suberized tuber parenchyma, providing a model system to study suberin production. Spatial and temporal coordination of SPPD and SPAD-related metabolism are required for suberization, as the former is produced soon after wounding, and the latter is synthesized later into wound-healing. Many steps involved in suberin biosynthesis remain uncharacterized, and the mechanism(s) that regulate …
Role Of Fatty Acid Omega-Hydroxylase 1 And Abscisic Acid In Potato Tuber Suberin Formation, Meg Haggitt
Role Of Fatty Acid Omega-Hydroxylase 1 And Abscisic Acid In Potato Tuber Suberin Formation, Meg Haggitt
Electronic Thesis and Dissertation Repository
Suberin is a complex biopolymer composed of two distinct but covalently-linked domains. The first domain is composed of polymerized phenolic monomers, whereas the second domain is predominately fatty acid derivatives esterified with glycerol. Deposited in specialized cells during development or in response to abiotic stress, suberin functions as a barrier to water loss and pathogen attack. In potato, more than 65% of suberin monomers undergo ω-hydroxylation, representing a major class of fatty acids in the final biopolymer. The ω-hydroxylation reaction is catalyzed by Cytochrome P450 (CYP) proteins, of which few have been characterized to date. In 2009, CYP86A33 from potato …
Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma
Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma
Electronic Thesis and Dissertation Repository
Part of the resistance mechanism of soybean (Glycine max [L.] Merr.) to Phytopthora sojae Kauf. & Gerd. involves pre-formed root suberin. In order to investigate the role of suberin in this host-pathogen interaction, I characterized hairy roots, formed as a result of Agrobacterium rhizogenes (Riker et al.) Conn infection, as a model to be used as a reliable soybean transformation system. I established hairy root cultures and demonstrated that they were a result of A. rhizogenes infection. The anatomy and suberin deposition in soybean hairy roots was examined, and found to be very similar to that of wild-type roots. …