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Full-Text Articles in Life Sciences

Roles Of Peroxisomes And Peroxisome-Derived Products In Controlling Plant Growth And Stress Responses, Elizabeth May Frick Dec 2017

Roles Of Peroxisomes And Peroxisome-Derived Products In Controlling Plant Growth And Stress Responses, Elizabeth May Frick

Arts & Sciences Electronic Theses and Dissertations

The peroxisome is a vital organelle conserved through the entire eukaryotic lineage. In all examined species, peroxisomes are responsible for such essential processes as fatty acid beta-oxidation and metabolism of reactive oxygen species (ROS). In plants, peroxisomes have taken on additional specialized roles, such as production of some plant hormones and vitamins. In this work, I have uncovered novel factors regulating peroxisome number in model species Arabidopsis thaliana, and novel mechanisms governing how peroxisomes respond to salt stress. I discovered a role for Arabidopsis MAP KINASE17 (MPK17) as a negative regulator of peroxisome division that acts in the salt-stress response …


Analysis Of Argonaute-Small Rna-Transcription Factor Circuits Controlling Leaf Development, John Steen Hoyer Dec 2017

Analysis Of Argonaute-Small Rna-Transcription Factor Circuits Controlling Leaf Development, John Steen Hoyer

Arts & Sciences Electronic Theses and Dissertations

Experimental studies of plant development have yielded many insights into gene regulation, revealing interactions between core transcriptional and post-transcriptional regulatory pathways present in all land plants. This work describes a direct connection between the three main small RNA-transcription factor circuits controlling leaf shape dynamics in the reference plant Arabidopsis thaliana. We used a high-throughput yeast 1-hybrid platform to identify factors directly binding the promoter of the highly specialized ARGONAUTE7 silencing factor. Two groups of developmentally significant microRNA-targeted transcription factors were the clearest hits from these screens, but transgenic complementation analysis indicated that their binding sites make only a small contribution …


Seeds As Artifacts Of Communities Of Practice: The Domestication Of Erect Knotweed In Eastern North America, Natalie Graham Mueller May 2017

Seeds As Artifacts Of Communities Of Practice: The Domestication Of Erect Knotweed In Eastern North America, Natalie Graham Mueller

Arts & Sciences Electronic Theses and Dissertations

Humans are the ultimate ecosystem engineers, and in transforming ecosystems we also change the selective environment for the plants and animals that live among us. The bodies and behaviors of domesticated plants and animals are thus rich artifacts of traditional ecological knowledge and practice. I study the morphology and behavior of domesticated plants as a proxy for ancient agricultural communities of practice. The transition from food procurement to food production is one of the most significant shifts in human history. I consider this process as the evolution and spread of a knowledge system. Domestication studies are usually focused on differentiating …