Life Sciences Commons^™

Changes In Corn Residue Quality Throughout The Grazing Period And Effect Of Supplementation Of Calves Grazing Corn Residue, Amanda J. Burken

Department of Animal Science: Dissertations, Theses, and Student Research

Corn residue is an abundant feed source in Nebraska that can be utilized as an alternative winter feed. Calves were backgrounded on corn residue in order to determine gain and estimate forage intake when supplemented with distillers grains (DGS). Calves grazing the non-irrigated field gained more (1.03 kg/calf daily) when compared to those grazing the irrigated field (0.90 kg/calf daily; P < 0.01). In year 1, a quadratic effect for intake of DGS was present (P < 0.01) while year 2 observed a linear effect for increasing level of DGS (P < 0.01). The nutritional quality of corn residue was evaluated over time in order to determine changes in blade/sheath, cob, husk/shank and stem. Minimal changes in DM of the forage components occurred was grain reached 15.5% moisture. Digestibility of the blade/sheath declined linearly over time (P < 0.01) while the husk remained constant (P = 0.40). Cob digestibility decreased quadratically (P < 0.01) throughout the sampling period with few changes once grain reached 15.5% moisture. Differences observed in the digestibility of the blade/sheath were attributed to the effects of weathering. A third set of trials was conducted to evaluate the effects of by-product supplementation of calves grazing irrigated corn residue and supplemented with DGS or continuous access to lick tubs. The DGS treatment gained more (0.62 kg/calf daily) than the lick tub treatment (0.38 kg/calf daily; P < 0.01). Calves offered DGS consumed more supplement as a percentage of BW (0.52%) when compared with calves offered lick tubs (0.36%; P < 0.01) on a DM basis. Calves supplemented with DGS had a higher supplement efficiency (46.3% to 42.9%, DM basis) although no differences were present between treatments (P = 0.49). When analyzed on an OM basis, however, calves offered lick tubs had a numerically higher supplement efficiency (50.4%) in comparison to calves …

Managing Threats To The Urban Forest: From Dutch Elm Disease To Emerald Ash Borer - Learning From Experience, Christopher J. Borman

Doctor of Plant Health Program: Dissertations and Student Research

The urban forest provides important essential services to all municipalities; however, its value is often overlooked. The urban forest contributes to energy savings, environmental benefits, psychological well-being, and social benefits. Managing the urban forest in a sustainable manner is important if we wish to benefit from these services well into the future. Reliable management techniques have been created through previous experiences with pests, and these should be utilized and improved for use on urban forests.

American elm (Ulmus americana L.) was once a major component of the urban forests of North America. In 1927, Dutch elm disease (DED) was …

Pseudomonas Syringae Type Iii Effectors: Targets And Roles In Plant Immunity, Tania Y. Toruño

Center for Plant Science Innovation: Faculty and Staff Publications

Pseudomonas syringae is a Gram-negative bacterial pathogen that infects many crops. A central virulence strategy P. syringae uses to successfully infect plants is the injection of type III effector proteins (T3Es) into plant cells through a type IIII protein secretion system (T3SS). The T3SS is a molecular syringe found in many Gram-negative bacterial pathogens of plants and animals that transport T3Es from the bacterial cytosol into eukaryotic cells. T3Es disrupt host processes in the plant immune system required to restrict pathogen ingress. The plant innate immune system is divided in two branches, pathogen-associated molecular patterns (PAMP)-triggered immunity (PTI) and effector-triggered …

Metabolic Engineering Of Oilseed Crops To Produce High Levels Of Novel Acetyl Glyceride Oils With Reduced Viscosity, Freezing Point And Calorific Value, Jinjie Liu, Adam Rice, Kathleen Mcglew, Vincent Shaw, Hyunwoo Park, Thomas E. Clemente, Mike Pollard, John Ohlrogge, Timothy P. Durrett

Department of Agronomy and Horticulture: Faculty Publications

Seed oils have proved recalcitrant to modification for the production of industrially useful lipids. Here, we demonstrate the successful metabolic engineering and subsequent field production of an oilseed crop with the highest accumulation of unusual oil achieved so far in transgenic plants. Previously, expression of the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene in wild-type Arabidopsis seeds resulted in the accumulation of 45 mol% of unusual 3-acetyl-1,2- diacyl-sn-glycerols (acetyl-TAGs) in the seed oil (Durrett et al., 2010 PNAS 107:9464). Expression of EaDAcT in dgat1 mutants compromised in their ability to synthesize regular triacylglycerols increased acetyl-TAGs to 65 mol%. Camelina and soybean …

First Report Of Goss's Bacterial Leaf Blight And Wilt Of Corn Caused By Clavibacter Michiganensis Subsp. Nebraskensis In North Dakota, A. Friskop, K. Kinzer, M. Mcconnell, Z. Liu, K. A. Korus, A. Timmerman, T. Jackson

Department of Plant Pathology: Faculty Publications

In August of 2011, the North Dakota State University Plant Diagnostic Lab received a hybrid corn (Zea mays) leaf sample from Burleigh County in southcentral North Dakota (ND). The leaf had long, irregular, water-soaked lesions consistent with Goss's leaf blight of corn. Using a light microscope at 10× magnification, bacterial streaming was observed from the excised edge of leaf tissue. A bacterial suspension was created, streaked onto a semi-selective CNS medium (1), and incubated at 22°C. Dark yellow-orange colonies appeared on the medium after 5 days. Single colonies were subcultured onto additional CNS media. To verify the identity …

Decision Support Tools To Address Climate Change: Climate Model - Land Surface Models, Zea Mays L. (Corn) Phenology And Evapotranspiration-Yield Sensitivity Models For Nebraska, Usa., Jane A. Okalebo

School of Natural Resources: Dissertations, Theses, and Student Research

Nebraska's climate is highly variable and is expected to change in the future with anthropogenic global warming (AGW), resulting in warmer spring and summer temperatures coupled with more erratic rainfall events. This has strong implications for agriculture in the region, yet it is not clear that current modeling and decision-support tools are adequate to address these looming changes and provide planning, mitigation and adaptation strategies. To address climate change and its implications to agriculture in Nebraska, a set of robust decision support tools are very crucial. This study herein are divided into three chapters, with each chapter addressing a specific …

Functional Genomics And Microbiome Profiling Of The Asian Longhorned Beetle (Anoplophora Glabripennis) Reveal Insights Into The Digestive Physiology And Nutritional Ecology Of Wood Feeding Beetles, Erin D. Scully, Scott M. Geib, John E. Carlson, Ming Tien, Duane Mckenna, Kelli Hoover

Department of Agronomy and Horticulture: Faculty Publications

Background: Wood-feeding beetles harbor an ecologically rich and taxonomically diverse assemblage of gut microbes that appear to promote survival in woody tissue, which is devoid of nitrogen and essential nutrients. Nevertheless, the contributions of these apparent symbionts to digestive physiology and nutritional ecology remain uncharacterized in most beetle lineages.

Results: Through parallel transcriptome profiling of beetle- and microbial- derived mRNAs, we demonstrate that the midgut microbiome of the Asian longhorned beetle (Anoplophora glabripennis), a member of the beetle family Cerambycidae, is enriched in biosynthetic pathways for the synthesis of essential amino acids, vitamins, and sterols. Consequently, the midgut …

The Effect Of Agricultural Practices On Sugar Beet Root Aphid (Pemphigus Betae Doane) And Beneficial Epigeal Arthropods, Rudolph J. Pretorius

Department of Entomology: Dissertations, Theses, and Student Research

This study investigated the effect of several agricultural practices on the sugar beet root aphid (Pemphigus betae Doane) and beneficial epigeal natural enemies in western Nebraska sugar beet agroecosystems. Eight glyphosate-tolerant sugar beet varieties were evaluated under field conditions for their resistance to root aphids. High levels of aphid resistance were detected for some varieties. In conjunction to this, pitfall sampling was conducted to determine the beneficial epigeal natural enemy complex in the area, which could contribute to the management of both root aphids and glyphosate-resistant weeds. Ground beetles comprised an important and abundant component of this fauna, with …

Litter Decomposition And Root Production In Response To Grazing On Sandhills Subirrigated Meadow, Ben W. Beckman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Stocking density can be manipulated by management factors including herd size, pasture size, and grazing period length. Ultrahigh stocking density (mob grazing) is commonly characterized at 200,000 kg animal live weight ha^-1 or higher with multiple movements of livestock per day. Practitioners claim increased plant production, soil development, and nutrient cycling rates because of high trampling percentages resulting from ultrahigh socking densities. This study was conducted on subirrigated meadow in the Nebraska Sandhills in 2012 and 2013 to determine grazing method effect on annual root production and litter decomposition. Stocking rates were held constant while stocking densities varied by …

Carbohydrate Metabolism And The Trehalose Biosynthetic Pathway In Maize Kernels Grown In Vitro Under Sucrose Starvation Stress, Samuel W. Bledsoe

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Drought is an increasing issue that many farmers encounter especially in hot arid climates with little rainfall. High temperatures and inadequate rainfall at certain stages in crop development can have disastrous consequences to yield. In maize, drought occurring near or during the flowering stage often causes significant kernel abortion that greatly impacts potential yield. The trehalose biosynthetic pathway has recently been found to be important in plant metabolism in response to stress in higher order plants. Trehalose is currently known throughout the plant and animal kingdoms as an osmoprotectant, high energy fuel source, structural component, and involved in pathogen response. …

2015 Spring Seed Guide, Teshome Regassa, Charles A. Shapiro

Department of Agronomy and Horticulture: Faculty Publications

NEBRASKA CORN HYBRID TESTS CROP PRODUCTION SUMMARY: According to the National Agricultural Statistics Service, there were 8.75 million acres of corn harvested in Nebraska in 2014 producing approximately 1.58 billion bushels of grain. The total average corn yield for Nebraska in 2014 was a record 181 bushels per acre (bu/a). Total corn yields from the previous 10 years are reported below.

NEBRASKA SOYBEAN VARIETY TESTS - 2014 - CROP PRODUCTION SUMMARY: According to the National Agricultural Statistics Service, there were 5.4 million acres of soybeans planted in Nebraska in 2014. 5.35 million acres were harvested producing around 288 million bushels. …

Comprehensive Characterization And Rna-Seq Profiling Of The Hd-Zip Transcription Factor Family In Soybean (Glycine Max) During Dehydration And Salt Stress, Vikas Belamkar, Nathan T. Weeks, Arvind K. Bharti, Andrew Farmer, Michelle A. Graham, Steven B. Cannon

Department of Agronomy and Horticulture: Faculty Publications

Background:

The homeodomain leucine zipper (HD-Zip) transcription factor family is one of the largest plant specific superfamilies, and includes genes with roles in modulation of plant growth and response to environmental stresses. Many HD-Zip genes are characterized in Arabidopsis (Arabidopsis thaliana), and members of the family are being investigated for abiotic stress responses in rice (Oryza sativa), maize (Zea mays), poplar (Populus trichocarpa) and cucumber (Cucmis sativus). Findings in these species suggest HD-Zip genes as high priority candidates for crop improvement.

Results:

In this study we have identified members of …

Chlorovirus Atcv-1 Is Part Of The Human Oropharyngeal Virome And Is Associated With Changes In Cognitive Functions In Humans And Mice, Robert H. Yolken, Lorraine Jones-Brando, David D. Dunigan, Geetha Kannan, Faith Dickerson, Emily Severance, Sarven Sabunciyan, C. Conover Talbot Jr., Emese Prandovszky, James R. Gurnon, Irina V. Agarkova, Flora Leister, Kristin L. Gressitt, Ou Chen, Bryan Deuber, Fangrui Ma, Mikhail V. Pletnikov, James L. Van Etten

James Van Etten Publications

Chloroviruses (family Phycodnaviridae) are large DNA viruses known to infect certain eukaryotic green algae and have not been previously shown to infect humans or to be part of the human virome. We unexpectedly found sequences homologous to the chlorovirus Acanthocystis turfacea chlorella virus 1 (ATCV-1) in a metagenomic analysis of DNA extracted from human oropharyngeal samples. These samples were obtained by throat swabs of adults without a psychiatric disorder or serious physical illness who were participating in a study that included measures of cognitive functioning. The presence of ATCV-1 DNA was confirmed by quantitative PCR with ATCV-1 DNA being …

Nebline, Nov./Dec. 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Tiny Tastes Can Total BIG CALORIES Over the Holiday Season

Food & Health

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Control Of Herbicide-Resistant Volunteer Corn In Herbicide-Resistant Soybean, Parminder Chahal

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Volunteer corn is a problem weed in soybean fields because it reduces yield and seed quality, and potentially harbors insects, pests, and diseases. Several pre-packaged herbicides have been registered in soybean in recent years, but response of volunteer corn to these herbicides has not yet been documented. Therefore, the first objective of this study was to evaluate the response of glufosinate-, glyphosate-, and imidazolinone-resistant volunteer corn to 20 pre-emergence (PRE) and 17 post-emergence (POST) soybean herbicides. The results indicated that PRE soybean herbicides partially controlled (< 80%) volunteer corn except clomazone, while acetyl CoA carboxylase (ACCase) inhibiting herbicides provided ≥ 85% control. Germination and emergence are critical stages in weed seed establishment and persistence. Scientific literature is not available about the factors affecting germination and emergence of volunteer corn. The second objective was to determine the effects of different environmental and agronomic factors on the germination and emergence of glyphosate-resistant hybrid and volunteer corn. The results indicated that response of hybrid and volunteer corn to majority of the variables tested was similar, suggesting that volunteer corn can germinate and emerge in a wide range of climatic conditions. Majority of growers control volunteer corn when it is visible above the soybean canopy, but this can results in early season competition with soybean. The third objective was to evaluate the impact of different densities of glyphosate-resistant volunteer corn at different control timings, and late season volunteer corn emergence on soybean yields. Late season volunteer corn emergence had no significant effect on soybean yield. Yield did not decrease with all volunteer corn densities, except with the highest density (10,000 plants and 500 clumps ha^-1) at all control timings. Soybean growers are looking for alternative herbicides, such …

South Loup River Valley Floristics: A Survey Of Selected Sites In Buffalo County, Nebraska, Steven J. Rothenberger, April M. Christman, Kevin Turek, Emmylou A. O'Brien

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Originating in the Nebraska Sand Hills, the South Loup River flows 30 km north of Kearney, Nebraska and traverses the northern edge of Buffalo County as it flows eastward to its confluence with the Middle Loup River in southwestern Howard County. Several diverse plant communities are associated with the South Loup Valley, including wet-meadows, wetland/oxbows, sand prairie, and riparian forest. Over a period of 6 years (2006-2012), we surveyed representative wet meadow, wetland, and sand prairie communities. We compiled an annotated vascular plant checklist that included 324 vascular plant species of which 11 were new county records. Three species of …

Genetic And Aggressiveness Variation Among Sclerotinia Sclerotiorum Dry Bean Isolates From Brazil Fields, Willian Luis Antonio Zancan, James R. Steadman, Rebecca Higgins, Rachana Jhala, Jose Da Cruz Machado

Department of Plant Pathology: Faculty Publications

Sclerotinia sclerotiorum, infection of bean fields, has increased in Brazil. Fungicides application is the control strategy used due to lack of cultivars with complete disease resistance. To guide the use of isolates in resistance screening 25 S. sclerotiorum isolates from Brazilian dry bean fields were characterized using microsatellite markers, mycelial compatibility groups (MCGs) and aggressiveness. Microsatellite primer pairs were used to identify polymorphisms among the S. sclerotiorum isolates and MCGs were determined from interaction of all isolates grown side by-side. Aggressiveness was derived from a straw test where fungal mycelium was placed over a cut bean stem and rated for …

An Eriophyid Mite-Transmitted Plant Virus Contains Eight Genomic Rna Segments With Unusual Heterogeneity In The Nucleocapsid Protein, Satyanarayana Tatineni, Anthony J. Mcmechan, Everlyne N. Wosula, Stephen N. Wegulo, Robert A. Graybosch, Roy French, Gary L. Hein

Department of Plant Pathology: Faculty Publications

Eriophyid mite-transmitted, multipartite, negative-sense RNA plant viruses with membrane-bound spherical virions are classified in the genus Emaravirus. We report here that the eriophyid mite-transmitted Wheat mosaic virus (WMoV), an Emaravirus, contains eight genomic RNA segments, the most in a known negative-sense RNA plant virus. Remarkably, two RNA 3 consensus sequences, encoding the nucleocapsid protein, were found with 12.5% sequence divergence, while no heterogeneity was observed in the consensus sequences of additional genomic RNA segments. The RNA-dependent RNA polymerase, glycoprotein precursor, nucleocapsid, and P4 proteins of WMoV exhibited limited sequence homology with the orthologous proteins of other emaraviruses, while …

Biosafety: Evaluation And Regulation Of Genetically Modified (Gm) Crops In The United States, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

This review of the safety assessment of genetically modified (GM) crops is focused primarily on the process and progress in the United States (US). It reviews the development of the safety evaluation process from the Asilomar conference in 1975 considering issues relevant to recombinant DNA technology, to discussions between the US government, academic and industrial scientists between 1984 and 1994 when the first GM crops were being field tested and evaluated commercial release for food and feed production. International guidelines were also reviewed for consistency with the US system. The overall process includes consideration of information relating to history of …

Overexpression Of Patatin-Related Phospholipase Aiiiβ Altered The Content And Composition Of Sphingolipids In Arabidopsis, Maoyin Li, Jennifer E. Markham, Xuemin Wang

Department of Biochemistry: Faculty Publications

In plants, fatty acids are primarily synthesized in plastids and then transported to the endoplasmic reticulum (ER) for synthesis of most of the complex membrane lipids, including glycerolipids and sphingolipids. The first step of sphingolipid synthesis, which uses a fatty acid and a serine as substrates, is critical for sphingolipid homeostasis; its disruption leads to an altered plant growth. Phospholipase As have been implicated in the trafficking of fatty acids from plastids to the ER. Previously, we found that overexpression of a patatin-related phospholipase, pPLAIIIβ, resulted in a smaller plant size and altered anisotropic cell expansion. Here, we determined the …

Nebline, October 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Smart Snacking

Food & Health

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Selection And Genetic Drift In North American Maize, Collin Lamkey

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Characterizing the impact of selection and genetic drift in the formation of heterotic groups and patterns in maize can reveal important insights into the mechanism underlying adaptation, and the relative importance of each force in defining population structure. The objectives were to characterize the role selection for hybrid performance had in defining population structure in both a reciprocal full-sib selection (RFS) program and a large collection of historically important inbred lines.

The Illumina GoldenGate Assay was used to genotype the University of Nebraska-Lincoln Replicated Recurrent Selection (UNL-RpRS) program. Eight cycles of S1-progeny and RFS selection were conducted for an index …

Chlorovirus Skp1-Binding Ankyrin Repeat Protein Interplay And Mimicry Of Cellular Ubiquitin Ligase Machinery, Eric A. Noel, Ming Kang, Jiri Adamec, James L. Van Etten, George A. Oyler

James Van Etten Publications

The ubiquitin-proteasome system is targeted by many viruses that have evolved strategies to redirect host ubiquitination machinery. Members of the genus Chlorovirus are proposed to share an ancestral lineage with a broader group of related viruses, nucleo-cytoplasmic large DNA viruses (NCLDV). Chloroviruses encode an Skp1 homolog and ankyrin repeat (ANK) proteins. Several chlorovirus-encoded ANK repeats contain C-terminal domains characteristic of cellular F-boxes or related NCLDV chordopox PRANC (pox protein repeats of ankyrin at C-terminal) domains. These observations suggested that this unique combination of Skp1 and ANK repeat proteins might form complexes analogous to the cellular Skp1-Cul1-F-box (SCF) ubiquitin ligase complex. …

Msh1-Induced Non-Genetic Variation Provides A Source Of Phenotypic Diversity In Sorghum Bicolor, Roberto De La Rosa Santamaria, Mon-Ray Shao, Guomei Wang, David O. Nino-Liu, Hardik Kundariya, Yashitola Wamboldt, Ismail M. Dweikat, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

MutS Homolog 1 (MSH1) encodes a plant-specific protein that functions in mitochondria and chloroplasts. We showed previously that disruption or suppression of the MSH1 gene results in a process of developmental reprogramming that is heritable and non-genetic in subsequent generations. In Arabidopsis, this developmental reprogramming process is accompanied by striking changes in gene expression of organellar and stress response genes. This developmentally reprogrammed state, when used in crossing, results in a range of variation for plant growth potential. Here we investigate the implications of MSH1 modulation in a crop species. We found that MSH1-mediated phenotypic variation in Sorghum bicolor …

Cold Frames, High Tunnels, And Greenhouses: Choose A Growing Structure Best For You, Stacy A. Adams, Kim A. Todd

Department of Agronomy and Horticulture: Faculty Publications

Cold frames, high tunnels, and greenhouses are plant growth structures that can be used to extend the growing season or allow for use year around for growing high value specialty crops. This publication discusses the various attributes and applications of each so that plant producers in teaching, research, extension, commercially or as a hobby can determine the best structure to meet individual needs.

Cold frames, high tunnels, and greenhouses are structures that provide hobbyists and growers with options for plant protection and season extension. They vary in cost, size, structural complexity, and ability for year-round usage. The small cold frame …

Nebline, September 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Youth Showcase Their Achievements at Super Fair

Food & Health

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The Trehalose Pathway In Maize: Conservation And Gene Regulation In Response To The Diurnal Cycle And Extended Darkness, Clémence Henry, Samuel W. Bledsoe, Allison Siekman, Alec Kollman, Brian M. Waters, Regina Feil, Mark Stitt, L. Mark Lagrimini

Department of Agronomy and Horticulture: Faculty Publications

Energy resources in plants are managed in continuously changing environments, such as changes occurring during the day/night cycle. Shading is an environmental disruption that decreases photosynthesis, compromises energy status, and impacts on crop productivity. The trehalose pathway plays a central but not well-defined role in maintaining energy balance. Here, we characterized the maize trehalose pathway genes and deciphered the impacts of the diurnal cycle and disruption of the day/night cycle on trehalose pathway gene expression and sugar metabolism. The maize genome encodes 14 trehalose-6-phosphate synthase (TPS) genes, 11 trehalose-6-phosphate phosphatase (TPP) genes, and one trehalase gene. Transcript abundance of most …

Contrasting Metabolism In Perenniating Structures Of Upland And Lowland Switchgrass Plants Late In The Growing Season, Nathan Palmer, Aaron J. Saathoff, Christian M. Tobias, Paul Twigg, Yuannan Xia, Kenneth P. Vogel, Soundararajan Madhavan, Scott E. Sattler, Gautam Sarath

Department of Agronomy and Horticulture: Faculty Publications

Background: Switchgrass (Panicum virgatum L.) is being developed as a bioenergy crop for many temperate regions of the world. One way to increase biomass yields is to move southern adapted lowland cultivars to more northern latitudes. However, many southerly adapted switchgrass germplasm can suffer significant winter kill in northerly climes.

Materials and Methods: Here, we have applied next-generation sequencing in combination with biochemical analyses to query the metabolism of crowns and rhizomes obtained from two contrasting switchgrass cultivars. Crowns and rhizomes from field-grown lowland (cv Kanlow) and upland (cv Summer) switchgrass cultivars were collected from three randomly selected post-flowering …

Diversity Of Bacteria Carried By Pinewood Nematode In Usa And Phylogenetic Comparison With Isolates From Other Countries, Diogo Neves Proença, Luís Fonseca, Thomas Powers, Isabel M.O. Abrantes, Paula V. Morais

Department of Plant Pathology: Faculty Publications

Pine wilt disease (PWD) is native to North America and has spread to Asia and Europe. Lately, mutualistic relationship has been suggested between the pinewood nematode (PWN), Bursaphelenchus xylophilus the causal nematode agent of PWD, and bacteria. In countries where PWN occurs, nematodes from diseased trees were reported to carry bacteria from several genera. However no data exists for the United States. The objective of this study was to evaluate the diversity of the bacterial community carried by B. xylophilus, isolated from different Pinus spp. with PWD in Nebraska, United States. The bacteria carried by PWN belonged to Gammaproteobacteria …

Bot Canker Pathogens Could Complicate The Management Of Phytophthora Black Pod Of Cocoa, Idowu Jaiyeola, Rufus J. Akinrinlola, Gbodope S. Ige, Oluwatimilehin O. Omoleye, Abiola Oyedele, Bayode J. Odunayo, Omotayo J. Emehin, Marcus O. Bello, Anthony O. Adesemoye

Department of Plant Pathology: Faculty Publications

Black pod is a major hindrance to cocoa production in Nigeria. It is caused by three different Phytophthora species with Phytophthora megakarya as the most important species in Nigeria and West African subregion. Phytophthora spp. may enhance infections by opportunistic pathogens such as members of the Botryosphaeriacea that cause branch and trunk cankers in many woody plants across the world. Botryosphaeriacea has not been reported in cocoa nor in any woody plants in Nigeria to our knowledge. In the cocoa belt of Nigeria, research and understanding on cocoa black pod and Phytophthora is limited partly because of delayed or no …