Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Selection Of Winter Wheat Mutant Lines Resistant To Drought Stress, Brian Kontz, Sean Franklin, Charles Brunel
Selection Of Winter Wheat Mutant Lines Resistant To Drought Stress, Brian Kontz, Sean Franklin, Charles Brunel
The Journal of Undergraduate Research
Mutated winter wheat lines were screened for improved drought resistance compared to the wildtype by withholding water for 3 weeks after 9 weeks of normal growth, and noting their responses upon re-watering. Of the 146 mutants grown, 73% were either killed due to water stress or showed poor recovery, while 27% recovered better than the wildtype line. This experiment enabled the selection of candidates for further investigation to better understand the genetic basis of drought tolerance in winter wheat.
Effects Of Stratification, Warm Treatment, And Mechanical And Acid Scarification On The Emergence Of Yellow-Flowered Alfalfa (Medicago Sativa Subsp. Falcata) Seeds, Diane Narem
The Journal of Undergraduate Research
Yellow-flowered alfalfa (YFA) has great potential to improve rangelands in the Northern Plains. Greater than 99% of YFA seeds from soil were viable but less than 4% germinated under standard laboratory conditions. The objective was to determine if low germination was due to physical or physiological seed dormancy. The experiment utilized a random complete block design with two trials, eight replications per trial. Each replication consisted of ten treatments plus a control, four stratification treatments consisting of a combination of two temperatures (-5°C, -20°C) and two durations (48 hr, 72 hr), one warm treatment (4 min, 60°C), four sandpaper scarification …
Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets
Time And Temperature Requirements For Effective Removal Of High Molecular Weight Rna From Winter Wheat Genomic Dna With Licl, Benjamin Meyerink, Ryan Geraets
The Journal of Undergraduate Research
Numerous protocols are available for the isolation of plant genomic DNA. Often times, these protocols utilize a wide variety of solutions. The primary purpose of this project was to examine the use of LiCl for the removal of RNA contaminants within genomic DNA samples and its dependency on incubation time and temperature. Our results indicate that LiCl is sufficient for the removal of high molecular weight RNA contaminants from genomic DNA. In addition, our results illustrate varying incubation times with LiCl yield minimal differences in the recovery of genomic DNA and the removal of RNA contaminants. Alternatively, different incubation temperatures …