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Full-Text Articles in Life Sciences
15n Effects On Chlamydomonas Reinhardtii, Ryan Geraets, Brett Kollars
15n Effects On Chlamydomonas Reinhardtii, Ryan Geraets, Brett Kollars
The Journal of Undergraduate Research
Non-radioactive isotopic labeling has become a regular technique for efficiently labeling a wide range of macromolecules. The overall goal for this project was to develop a method of globally measuring plant protein turnover rates. In order to do so, an isotopic labeled environment that does not induce stress had to be used. The objective of this particular segment of research was to develop a 15N-labeled Tris-Acetate-Phosphate (TAP) media in which Chlamydomonas reinhardtii can successfully grow without eliciting stressed physiological responses. Our results illustrate that we have successfully developed 15N-labeled TAP that does not stress Chlamydomonas reinhardtii.
Effects Of Deuterium On Chlamydomonas Reinhardtii, Brett Kollars, Ryan Geraets
Effects Of Deuterium On Chlamydomonas Reinhardtii, Brett Kollars, Ryan Geraets
The Journal of Undergraduate Research
In order to develop software tools to monitor rates of total protein turnover in plants, attempts were made to maximally label all the proteins in vivo. Our study focused on the use of the stable isotope of hydrogen, deuterium, to label proteins of C. reinhardtii. The goal of this study was to determine the effect of the label on algal growth. C. reinhardtii is a unicellular green algae that is heterotrophic, photoautotrophic and mixotrophic. The results showed that increasing concentrations of deuterium in the algal growth medium had a negative effect on the growth of the algae. These preliminary results …