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Articles 1 - 17 of 17
Full-Text Articles in Life Sciences
Pasturelands As Natural Climate Solutions: A Socioecological Study Of Tree Carbon And Beef Production Trade-Offs, Bela Starinchak
Pasturelands As Natural Climate Solutions: A Socioecological Study Of Tree Carbon And Beef Production Trade-Offs, Bela Starinchak
Masters Theses, 2020-current
Forest restoration is the most effective natural climate solution, with the potential to sequester 37% of the carbon dioxide (CO2) needed to reach the Paris climate mitigation goal. Cattle pastures offer an underutilized opportunity to increase global forest restoration efforts, improve biodiversity, and maximize carbon storage through the adoption of management strategies that prioritize the incorporation of trees into pasturelands. However, remote estimations of tree carbon storage in pastoral systems have never been field-verified and their accuracy is unclear. Furthermore, the effect of increased trees on cattle production is understudied across biomes. Lastly, the restoration potential of these …
Plant-Pollinator Network Dynamics Of The Little Fork Shale Barren, Sarah N. Brown
Plant-Pollinator Network Dynamics Of The Little Fork Shale Barren, Sarah N. Brown
Masters Theses, 2020-current
Shale barrens are steep sloping mountainside ecosystems characterized by rocky Upper Devonian age shale substrate, high light, and low water availability. They form an array of biogeographical “islands” throughout Mid-Appalachia whose niche dynamics, response to disturbance, and pollination ecology remain to be investigated. Using network analysis, this project addresses three objectives to fill gaps in shale barren pollination ecology. (i) Compare vegetative species composition, richness, diversity, and evenness to a descriptive vegetation study completed at the same site 27 years prior. ii) Outline the topology of plant-pollinator networks including identifying phenologically accurate networks, the architecture of such networks, and identifying …
Characterizing The Role Of Β-Amylase3 In Cold Stress Response And Recovery In Arabidopsis Thaliana, Isabelle G. Houston
Characterizing The Role Of Β-Amylase3 In Cold Stress Response And Recovery In Arabidopsis Thaliana, Isabelle G. Houston
Senior Honors Projects, 2020-current
Starch is a polymer of glucose that is used as an energy store in plants. Mobilization of starch has implications in abiotic stress survival and recovery. While the importance of carbon and energy allocation in plant survival has been explored, the specific roles of starch degrading enzymes in plant responses to stress are still unclear. β-Amylase3, or BAM3, is the principle starch degrading enzyme at night and is transcriptionally upregulated in response to cold stress in the plant Arabidopsis thaliana. Using single and quadruple knockout mutant plants, I aimed to clarify the role of BAM3 in the response to …
Modeling Species Distribution And Habitat Suitability Of American Ginseng (Panax Quinquefolius) In Virginia, Jacob D. J. Peters
Modeling Species Distribution And Habitat Suitability Of American Ginseng (Panax Quinquefolius) In Virginia, Jacob D. J. Peters
Masters Theses, 2020-current
American ginseng (Panax quinquefolius) is a well-known and sought-after medicinal plant native to North America that is facing increased threat of extinction due to overharvesting, herbivory, and habitat loss. Species distribution and habitat suitability models may be valuable to landowners interested in sustainable harvest or to institutions interested in the conservation and restoration of the species. With unequal sampling efforts across a region of interest, it is likely that some locations with appropriate habitat may be misrepresented in model predictions. This study refined a state-derived species distribution model for ginseng through increased sampling effort across the Cumberland Plateau …
In Vitro Propagation Of A Critically Endangered African Violets (Saintpaulia Rupicola B.L. Burtt), Thomas W. Lowry Iii
In Vitro Propagation Of A Critically Endangered African Violets (Saintpaulia Rupicola B.L. Burtt), Thomas W. Lowry Iii
Masters Theses, 2010-2019
The in vitro propagation is a promising method for the production of plants in species that are endangered, commercially valuable, and/or otherwise difficult to propagate through traditional horticultural methods. Leaf and petiole explants of critically endangered African violets (Saintpaulia rupicola B.L. Burtt) were cultured on a chemically defined medium that was supplemented with six different concentrations of thidiazuron (TDZ). The concentrations were used for either an induction medium on which the explants remained throughout development, or an induction medium on which the explants remained for 10 days followed by a basal growth medium for the remainder of the 12 …
Sustainable Agriculture: Integration Of Aquaponics At Punta Leona Hotel And Club In Costa Rica, Cailin Sierra Dyer, Paris Riley Smith
Sustainable Agriculture: Integration Of Aquaponics At Punta Leona Hotel And Club In Costa Rica, Cailin Sierra Dyer, Paris Riley Smith
Senior Honors Projects, 2010-2019
Sustainable agriculture is becoming an increasingly important method of food production. As human populations continue to grow, attendant food demand has been increasingly met via agribusiness, including monoculture crop production and factory farming. As is well documented, the rise of agribusiness has led to resource degradation and declining stocks on which “sustainable agriculture” relies. This paper describes a local attempt to re-establish “sustainable agriculture” through the development of an aquaculture system that mimics a naturally occurring cycle that integrates fish and plants. The system was constructed over a three-week period in Punta Leona, Costa Rica. First, the ground was cleared …
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase9 Using Yeast 2-Hybridization, Sheikh Hossain
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase9 Using Yeast 2-Hybridization, Sheikh Hossain
Senior Honors Projects, 2010-2019
In plants, starch is a major carbon and energy storage compound. Starch is made as a product of photosynthesis while plants are in light and is degraded at night. Our lab is interested in the cellular mechanism of starch degradation in plants and for our studies we use Arabidopsis thaliana as a model. β-amylases are primarily responsible for the hydrolysis of starch in plants and a total of nine β-amylases genes are encoded in Arabidopsis thaliana. These nine genes are identified as BAM1-9. BAM9 is located in the chloroplast where starch is located, and is present …
The Combined Effects Of Soil Fertility And Soil Amendments On The Growth And Mycorrhizal Associations Of American Ginseng (Panax Quinquefolius), Emily Ann Murray
The Combined Effects Of Soil Fertility And Soil Amendments On The Growth And Mycorrhizal Associations Of American Ginseng (Panax Quinquefolius), Emily Ann Murray
Masters Theses, 2010-2019
Arbuscular mycorrhizal fungi (AMF) are fungi that form symbiotic associations with 70-90% of plant families. They are known to allow for the extension of the root system as well as an increase in plant size by assisting with the uptake of nutrients such as nitrogen and phosphorus. The role that AMF play in plant health and success has led to the development of commercial inoculum, which is used in agricultural settings. However, soil fertility, and soil amendments are known to affect AMF and plant associations. This study intends to look at how cultivated American Ginseng seedlings are affected by commercial …
Investigating The Ecology Of A Rare Species On St. John, Usvi: Reintroducing Solanum Conocarpum In Light Of Climate Change, Cecilia Rogers
Investigating The Ecology Of A Rare Species On St. John, Usvi: Reintroducing Solanum Conocarpum In Light Of Climate Change, Cecilia Rogers
Senior Honors Projects, 2010-2019
Approximately two thirds of St. John is National Park territory. However, the land has been threatened with tourism and development, greatly impacting island biodiversity. One species that may become extinct due to this degradation is Solanum conocarpum. S. conocarpum is a rare shrub, endemic to the dry tropical forests of St. John, USVI. This plant is a species of conservation concern and is one of very few native and endemic plants on this island. Very little is known about the ecology and reproduction of S. conocarpum. Most plants are found on the southern half of the island. Recent observations …
Effects Of The Microenvironment Surrounding Cys433 In Arabidopsis Β-Amylase-1 And -3 On The Sensitivity To Glutathionylation By Nitrosoglutathione, Matthew R. Kohler
Effects Of The Microenvironment Surrounding Cys433 In Arabidopsis Β-Amylase-1 And -3 On The Sensitivity To Glutathionylation By Nitrosoglutathione, Matthew R. Kohler
Senior Honors Projects, 2010-2019
Glutathionylation is a reversible post-translational modification of proteins involving the transfer of glutathione to the thiols of specific cysteine residues. While the mechanism behind glutathionylation is known, the specificity of cysteine glutathionylation is not understood. It is known, however, that the two main factors affecting the susceptibility to glutathionylation are the reactivity and accessibility of cysteines in proteins, which is determined by the microenvironment. Using β-amylases (BAMs) 1 and 3 from Arabidopsis thaliana, which have different sensitivities to nitrosoglutathione (GSNO), as a model, I attempted to provide insight into why some cysteines are glutathionylated by GSNO and others are …
Using Ancient Practices To Fix Modern Problems: The Effect Of Biochar On Einkorn Wheat And Soil Quality, Sarah L. Miller
Using Ancient Practices To Fix Modern Problems: The Effect Of Biochar On Einkorn Wheat And Soil Quality, Sarah L. Miller
Senior Honors Projects, 2010-2019
As farmland soils become more and more depleted, the importance of effective soil amendments grows. Biochar is a potential soil and carbon amendment that could improve water and nutrient holding capacity and foster growth of beneficial microbes and fungi. Biochar does not contain nutrients but acts like a sponge, absorbing nutrients around it. It is so effective at holding nutrients that in the first year, if applied alone, it can make the nutrients unavailable to plants and lowers crop yields. To get the best results biochar must be saturated, also referred to as inoculated or charged, with nutrients. Once the …
The Effect Of Cattle Management On Soil Carbon: Implications For Climate Change, Kelly M. Livernoche
The Effect Of Cattle Management On Soil Carbon: Implications For Climate Change, Kelly M. Livernoche
Masters Theses, 2010-2019
In naturally occurring ecosystems, forests function as substantial carbon sinks, storing carbon in soil and in biomass that would otherwise exist in the atmosphere as carbon dioxide. The conversion of forested land to cattle pastures and their associated operational processes are noteworthy contributors to recent increases in global carbon emissions and subsequent climate change. However, appropriately managed cattle pastures have potential to be reservoirs for carbon. Rotational cattle pastures, where cattle are moved between enclosed sections of pasture, may improve soil carbon content compared to conventional practices. In rotational cattle pastures, a more even distribution of manure increases plant biomass, …
Impact Of Burkholderia Phytofirmans Strain Psjn On The Ex Vitro Acclimatization And In Vitro Propagation Of Tissue Cultured Dionaea Muscipula (Venus Fly Trap), Peter L. Riley
Masters Theses, 2010-2019
Dionaea muscipula, Venus fly trap, is an endangered plant that is propagated primarily through tissue culture. Tissue cultured plants must be acclimatized to their new environments when removed from their test tubes and the ensuing stress can result in plant mortality. A plant growth promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, has been utilized in biotic hardening of tissue cultured plants and in the amelioration of stresses, particularly drought stress in potted plants. We investigated whether this bacterium could improve either propagation or acclimatization of tissue cultured Venus fly traps. Standardized inoculations were performed by adjusting cell density of inoculant …
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase2 Using Yeast 2-Hybridization, Megan E. Hines
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase2 Using Yeast 2-Hybridization, Megan E. Hines
Senior Honors Projects, 2010-2019
BAM2 is a chloroplast-targeted member of the β-amylase gene family that currently has an unknown function in starch hydrolysis. Previous research indicated that BAM2 did not have significant catalytic activity, but, because the gene is highly conserved and there is a starch-excess phenotype in older plants lacking BAM2, it was hypothesized that BAM2 may instead have a regulatory function. Many regulatory proteins function by interacting with other proteins, so we wanted to test for potential protein binding partners for BAM2 using the yeast two-hybrid system. A plasmid containing the BAM2 gene from Arabidopsis thaliana was co-transformed into Y190-strain yeast ( …
Compost Land Management And Soil Carbon Sequestration, Kylene A. Hohman
Compost Land Management And Soil Carbon Sequestration, Kylene A. Hohman
Senior Honors Projects, 2010-2019
Extensive fossil fuel burning has released carbon dioxide into the atmosphere. Under proper ecological conditions plants convert atmospheric carbon dioxide into stable soil organic matter, a natural and efficient means of mitigating climate change. In the symbiotic relationship between mycorrhizae and plants, mycorrhizae provide plants with essential nutrients in exchange for carbon sugars leaked from the plants. Mycorrhizae convert carbon sugars to an exudate called glomalin, a protein that assists in developing soil aggregates composed of sand, silt, and clay. These aggregates, called humus, store carbon for hundreds of years under healthy ecological conditions. Compost prompts soil microbes to aerobically …
Using Morphometrics To Evaluate Taxonomic Relationships In Eleocharis Tenuis (Cyperaceae), Lane D. Gibbons
Using Morphometrics To Evaluate Taxonomic Relationships In Eleocharis Tenuis (Cyperaceae), Lane D. Gibbons
Masters Theses, 2010-2019
North America is host to multiple complex taxa, and unresolved relationships in the genus Eleocharis (Cyperaceae). Of noteworthy significance, are lasting taxonomic uncertainties involving plants referable to E. tenuis (var. tenuis, var. verrucosa, and var. pseudoptera) of the E. tenuis species complex. Morphology of E. tenuis is traditionally recognized as being widely variable, and intermediate among taxa along a broad geographic gradient. Cytological and morphometric data however, indicate that the varieties of E. tenuis are unique in cytology, and are consistently delimited by five morphological characters. Furthermore, this study shows that cytological data correlates with unique morphological …
The Relationship Between Riparian Zone Width And Floristic Quality In Shenandoah County, Virginia, Jamie D. Smith
The Relationship Between Riparian Zone Width And Floristic Quality In Shenandoah County, Virginia, Jamie D. Smith
Masters Theses, 2010-2019
Riparian zones harbor an above average plant biodiversity. This biodiversity is threatened by invasive species and increasing human disturbance, the latter of which includes deforestation from agriculture and urban development. In this study, I examine relationship between the width of a forested riparian zone and the vegetation growing there. By using floristic quality assessment as a measure of anthropogenic disturbance, one can determine if wider riparian zones foster exclusion of non-native species while providing higher quality habitats for native plants. A randomized block design was used with three forested riparian treatments: deforested, moderately forested (woody vegetationstream), and extensively forested (woody …