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Full-Text Articles in Life Sciences
Dct4—A New Member Of The Dicarboxylate Transporter Family In C4 Grasse, Sarit Weissmann, Pu Huang, Madeline A. Wiechert, Koki Furuyama, Thomas P. Brutnell, Mitsutaka Taniguchi, James C. Schnable, Todd C. Mockler
Dct4—A New Member Of The Dicarboxylate Transporter Family In C4 Grasse, Sarit Weissmann, Pu Huang, Madeline A. Wiechert, Koki Furuyama, Thomas P. Brutnell, Mitsutaka Taniguchi, James C. Schnable, Todd C. Mockler
Center for Plant Science Innovation: Faculty and Staff Publications
Malate transport shuttles atmospheric carbon into the Calvin–Benson cycle during NADP-ME C4 photosynthesis. Previous characterizations of several plant dicarboxylate transporters (DCT) showed that they efficiently exchange malate across membranes. Here, we identify and characterize a previously unknown member of the DCT family, DCT4, in Sorghum bicolor. We show that SbDCT4 exchangesmalateacrossmembranesanditsexpressionpatternisconsistentwitharoleinmalatetransportduringC4 photosynthesis. SbDCT4 is not syntenic to the characterized photosynthetic gene ZmDCT2, and an ortholog is not detectable in the maize reference genome. We found that the expression patterns of DCT family genes in the leaves of Zea mays, and S. bicolor varied by cell type. Our results suggest that …
Isoseq Transcriptome Assembly Of C3 Panicoid Grasses Provides Tools To Study Evolutionary Change In The Panicoideae, Daniel S. Carvalho, Aime V. Nishimwe, James C. Schnable
Isoseq Transcriptome Assembly Of C3 Panicoid Grasses Provides Tools To Study Evolutionary Change In The Panicoideae, Daniel S. Carvalho, Aime V. Nishimwe, James C. Schnable
Center for Plant Science Innovation: Faculty and Staff Publications
The number of plant species with genomic and transcriptomic data has been increasing rapidly. The grasses—Poaceae—have been well represented among species with published reference genomes. However, as a result the genomes of wild grasses are less frequently targeted by sequencing efforts. Sequence data from wild relatives of crop species in the grasses can aid the study of domestication, gene discovery for breeding and crop improvement, and improve our understanding of the evolution of C4 photosynthesis. Here, we used long-read sequencing technology to characterize the transcriptomes of three C3 panicoid grass species: Dichanthelium oligosanthes, Chasmanthium laxum, and …