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Full-Text Articles in Life Sciences

Virus-Induced Changes In Nuclear Proteins And Membranes In Nicotiana Benthamiana Cells, Caleb Mathias Jan 2020

Virus-Induced Changes In Nuclear Proteins And Membranes In Nicotiana Benthamiana Cells, Caleb Mathias

Theses and Dissertations--Plant Pathology

Viruses rely on host proteins to complete their life cycles. This results in alterations to normal cell physiology to processes which benefit viral processes such as replication and movement to other cells. This may involve the relocalization of host proteins away from their original subcellular targets to sites which may benefit the virus, a process that is not as well understood as it relates to the nucleus. To identify nuclear proteins that may be involved in such processes, a library of random Nicotiana benthamiana cDNAs were expressed as GFP fusions in a transgenic marker lines expressing a histone 2B:RFP nuclear …


Characterizing The Roles Of Pipecolic Acid And Reactive Oxygen Species Metabolic Enzymes In Plant Systemic Immunity, Ruiying Liu Jan 2020

Characterizing The Roles Of Pipecolic Acid And Reactive Oxygen Species Metabolic Enzymes In Plant Systemic Immunity, Ruiying Liu

Theses and Dissertations--Plant Pathology

Systemic acquired resistance (SAR), initiated by a plant upon recognition of microbial effectors, involves the generation of mobile signals at the primary infection site, which translocate to and activate defense responses in distal tissues. Among the signals contributing to SAR include salicylic acid (SA), nitric oxide (NO), reactive oxygen species (ROS), glycerol-3-phosphate (G3P), and pipecolic acid (Pip). Our previous studies show there are two branches of SAR signaling pathways in Arabidopsis: one regulated by NO/ROS-G3P and the other by SA. Both NO/ROS-G3P and SA-mediated signaling branches function in parallel during SAR. To better understand the role of Pip in SAR …


Pathogenicity, Toxigenic Potential, And Genomics Of Fusarium Graminearum And F. Meridionale Causing Ear And Stalk Rot Of Maize, Franklin Jackson Machado Jan 2020

Pathogenicity, Toxigenic Potential, And Genomics Of Fusarium Graminearum And F. Meridionale Causing Ear And Stalk Rot Of Maize, Franklin Jackson Machado

Theses and Dissertations--Plant Pathology

Gibberella ear (GER) and stalk rot (GSR) diseases of maize in Brazil are caused mainly by Fusarium meridionale, a species belonging to the Fusarium graminearum species complex (FGSC). Another species within this complex, F. graminearum sensu stricto (hereafter F. graminearum), is second in importance on maize, but is the most common species found causing Fusarium Head Blight disease of wheat in Brazil. The latter species is the predominant cause of GER and GSR in North America, where F. meridionale has not been found thus far. In this dissertation I undertook a comparative analysis of pathogenic, saprophytic, toxigenic and …


Diversity And Cross-Infection Potential Of Colletotrichum On Apples And Small Fruits In Kentucky Mixed-Fruit Orchards, Madison Julia Eaton Jan 2020

Diversity And Cross-Infection Potential Of Colletotrichum On Apples And Small Fruits In Kentucky Mixed-Fruit Orchards, Madison Julia Eaton

Theses and Dissertations--Plant Pathology

Fungi in the genus Colletotrichum cause apple, blueberry, and strawberry fruit rots, which result in significant losses for Kentucky growers. Most orchards in Kentucky are agritourism-focused and grow multiple fruits in close proximity. These mixed-fruit orchards may facilitate Colletotrichum cross-infection, which has serious management implications. Small fruit and apple Colletotrichum isolates from Kentucky orchards were characterized by morphotype, phylogenetic species identification, cross-inoculation, genome sequencing, and telomere fingerprinting. The small fruit isolates grouped into seven morphotypes, representing two species complexes: C. acutatum and C. gloeosporioides. All blueberry isolates belonged to the species C. fioriniae, and the majority of strawberry …