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Grapevine Vein Clearing Virus: Epidemiological Patterns And Construction Of A Clone, Cory Von Keith
Grapevine Vein Clearing Virus: Epidemiological Patterns And Construction Of A Clone, Cory Von Keith
MSU Graduate Theses
Grapevine vein clearing virus (GVCV) is a recently discovered virus belonging to the Badnavirus genus. Characteristic to its name, the virus is associated with a disease where symptoms manifest as pronounced vein-clearing, resulting in severe berry deformation and vine decline in susceptible grape varieties. Sustainable production of wine is dependent on healthy plants. The associated disease is mainly found in Midwest vineyards. Attempts were made in this thesis to provide evidence of causality of the virus to the associated disease and to infer the historical path and migration pattern of GVCV. Conclusions and discussions will provide grape producers with the …
Assessment Of Resistance To Botrytis Cinerea In Arabidopsis Expressing Grapevine Sts Genes And Analysis Of New Grapevine Vein Clearing Virus Isolates, Li Su
MSU Graduate Theses
Grapevine stilbene synthase gene (STS) family is unusually large with more than 32 members of full-length genes. Resveratrol and piceid are synthesized in VaSTS7-, VvSTS22-, VaSTS22-transgenic Arabidopsis. To study the function of these STS genes in the resistance against Botrytis cinerea in vivo, lesion sizes on these STS transgenic Arabidopsis were compared to that on non-transgenic control Col-0, at 96 hours post inoculation with B. cinerea spores at 0, 200, or 1,000 spores/μl. In each experiment there were three biological replicates. Three lesion sizes from each replicate were averaged. The experiment was repeated three …