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Degradation Of Wheat Streak Mosaic Virus Capsid Protein During Leaf Senescence, Myron K. Brakke, Rose N. Skopp, L. C. Lane

Department of Plant Pathology: Faculty Publications

Wheat streak mosaic virus capsid protein degraded in vivo by proteolysis as leaves senesced. The capsid protein of virus purified from young systemically infected leaves had an apparent size or 45 kDa in 12% sodium dodecyl sulfate-polyacrylamide gels with minor amounts of 43, 42, 33, and 3E kDa proteins. The proportion of smaller proteins increased with the age of the leaf. In some virus preparations only 3 1 kDa capsid protein was detected. In vitro proteolysis of virions with 45 kDa protein produced virions with 31 kDa protein. Virions with 31 kDa capsid protein sedimented slightly more slowly than those …