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A Unique Role For The Host Escrt Proteins In Replication Of Tomato Bushy Stunt Virus, Daniel Barajas, Yi Jiang, Peter D. Nagy
A Unique Role For The Host Escrt Proteins In Replication Of Tomato Bushy Stunt Virus, Daniel Barajas, Yi Jiang, Peter D. Nagy
Plant Pathology Faculty Publications
Plus-stranded RNA viruses replicate in infected cells by assembling viral replicase complexes consisting of viral- and host-coded proteins. Previous genome-wide screens with Tomato bushy stunt tombusvirus (TBSV) in a yeast model host revealed the involvement of seven ESCRT (endosomal sorting complexes required for transport) proteins in viral replication. In this paper, we show that the expression of dominant negative Vps23p, Vps24p, Snf7p, and Vps4p ESCRT factors inhibited virus replication in the plant host, suggesting that tombusviruses co-opt selected ESCRT proteins for the assembly of the viral replicase complex. We also show that TBSV p33 replication protein interacts with Vps23p ESCRT-I …