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Full-Text Articles in Life Sciences
Alteration Of Condensed Tannin Sythesis In Transgenic Forage Legumes, F Paolocci, G Tanner, S Arcioni, F Damiani
Alteration Of Condensed Tannin Sythesis In Transgenic Forage Legumes, F Paolocci, G Tanner, S Arcioni, F Damiani
IGC Proceedings (1997-2023)
The transformation of Lotus corniculatus plants with the maize gene Sn, reorganizes the tissue specificity of condensed tannins accumulation. In particular the transformed plants show an increase of tannin content in roots and a decrease in leaves. Molecular and enzymatic analyses suggest that the transgene can functionally substitute an endogenous unknown gene not expressed in roots and induces its silencing when it is expressed. These findings could have applications for reducing tannin content in unpalatable plants and for cloning genes involved in tannin synthesis.
Nutritional Implications Of Bound Proanthocyanidins, H P.S Makkar, K Becker
Nutritional Implications Of Bound Proanthocyanidins, H P.S Makkar, K Becker
IGC Proceedings (1997-2023)
The correlation between protein binding capacity of proanthocyanidin-rich neutral detergent fiber (NDF) and their proanthocyanidin (PAs) content was very low (r = -0.21; n = 6). These PAs-rich NDF did not affect growth of a tannin-sensitive strain of Clostridium perfringens. A substantial amount of bound PAs (75 to 92 %) disappeared from the NDF in 24 h of fermentation in an in vitro incubation medium containing rumen microbes. Addition of a tannin-binding agent, polyethylene glycol to the incubation medium containing rumen microbes and browses made free of extractable tannins or NDF rich in bound PAs increased the gas production up …
Genetic Manipulation Of Sainfoin Leaf Tannins, M Y. Gruber, P Auser, G Lees, A D. Muir, M Wood
Genetic Manipulation Of Sainfoin Leaf Tannins, M Y. Gruber, P Auser, G Lees, A D. Muir, M Wood
IGC Proceedings (1997-2023)
An isogenic population of sainfoin (Onobrychis viciifolia Scop.) plants was developed by two methods; regeneration after long term tissue culture and plant transformation with an antisense chalcone synthase gene. Tannin (proanthocyanidin) content could be dramatically increased in untransformed callus by long term culture, but was not significantly altered in regenerating plantlets. Some of the mature transgenic plants had dramatically reduced levels of leaf tannin. The antisense gene was eliminated from all transgenic plants later in development.
Induction Of Mutants With Ectopic Expression Of Condensed Tannins, M Y. Gruber, B Skadhauge, P Auser, A D. Muir, K K. Thomsen, J Stougaard, B Coulman, D Von Wettstein
Induction Of Mutants With Ectopic Expression Of Condensed Tannins, M Y. Gruber, B Skadhauge, P Auser, A D. Muir, K K. Thomsen, J Stougaard, B Coulman, D Von Wettstein
IGC Proceedings (1997-2023)
Leaves of 47,000 Lotus japonicus plants were screened using a butanol:HCl histochemical test to select “gain of function” mutants. These plants were progeny from L. japonicus lines which were transformed with T-DNA constructs containing either the maize Ds or Ac transposon (Thykjaer et al., 1995). Among 21 putative leaf tannin mutants, five (tan1-5) were characterized for synthesis of condensed tannins, leucocyanidin reductase activity and the presence of Ac and the selectable marker gene, nptII . A range of leaf tannin content among other Lotus species was also characterized.
The Effect Of Condensed Tannins In Lotus Spp. On The Lesser Migratory Grasshopper, C F. Hinks, G L. Lees, M Y. Gruber, A D. Muir, J Soroka
The Effect Of Condensed Tannins In Lotus Spp. On The Lesser Migratory Grasshopper, C F. Hinks, G L. Lees, M Y. Gruber, A D. Muir, J Soroka
IGC Proceedings (1997-2023)
Hatchling grasshoppers (Melanoplus sanguinipes Fab.) fed one accession of field grown big trefoil (Lotus uliginosis Schkuhr) containing a tannin content of 56 mg. g-1 FW, had a significantly lower mean weight than hatchlings fed field grown Lotus species and accessions with lower tannin content. Adult female grasshoppers fed L. uliginosis foliage with 32.3 mg.g-1 FW tannin also ate more feed and had a 2-fold higher gut trypsin and chymotrypsin activity than adults fed low tannin L. corniculatus foliage (0.13 mg.g-1 FW tannin). In more controlled experiments, growth at 20o C or 30o C was used to increase tannin …
Genotypic Effects On Condensed Tannins In The Leucaena Genus, S A. Dalzell, H M. Shelton
Genotypic Effects On Condensed Tannins In The Leucaena Genus, S A. Dalzell, H M. Shelton
IGC Proceedings (1997-2023)
One hundred and eighteen accessions of the Leucaena genus were assayed for extractable and bound condensed tannins (CT). Leucaena taxa varied from low or no CT (0-1%) to extremely high levels (>15%). There was considerable intraspecific variation in CT within key taxa. The proportion of bound tannin decreased with increasing total CT content.
Effects Of Incorporation Of Sainfoin (Onobrychis Viciafolia Scop.) With Cool Season Grasses On In Vitro Digestibility And Ch4 Emission, H. P. Poudel, Y. Wang, T. Mcallister, Z. J. Zu, B. Eisenreich, S. Acharya
Effects Of Incorporation Of Sainfoin (Onobrychis Viciafolia Scop.) With Cool Season Grasses On In Vitro Digestibility And Ch4 Emission, H. P. Poudel, Y. Wang, T. Mcallister, Z. J. Zu, B. Eisenreich, S. Acharya
IGC Proceedings (1997-2023)
Sainfoin (Onobrychis viciafolia Scop.) is an important non-bloating perennial leguminous forage. The tannins in sainfoin alter protein metabolism in the rumen and have been implicated in altering both nitrous oxide and methane emissions. However, the effect of sainfoin when mixed with cool-season forages is unknown. Therefore, in this study, we evaluated the in-vitro fermentation of sainfoin hay mixed with two other perennial cool-season forages, meadow bromegrass and orchardgrass at five ratios (0:100, 25:75, 50:50, 75:50, and 100:0). Data on dry matter disappearance (DMD), neutral detergent fiber disappearance (NDFD), gas production (GP) methane (CH4) emissions, and ammonia production were collected …
Tannins In Perennial Legume And Forb Functional Forages, J. Macadam, Juan Villalba, S. Lagrange, E. Stewart, S. Hunt, J. Legako, R. Christensen, R. L. Pitcher, A. Bolletta
Tannins In Perennial Legume And Forb Functional Forages, J. Macadam, Juan Villalba, S. Lagrange, E. Stewart, S. Hunt, J. Legako, R. Christensen, R. L. Pitcher, A. Bolletta
IGC Proceedings (1997-2023)
Feed is the greatest input cost for cattle producers. The studies summarized here employed non-bloating, tannin-containing irrigated perennial legume pastures or hay of legumes or a hydrolysable tannin-containing forb that were grown in the Mountain West USA, with non-tannin legume, grass, or feedlot treatments for comparison. Cattle grazing legume pastures or fed legume or forb hays had greater intake, gain and nitrogen retention, and in some cases, reduced enteric methane emissions compared with grass pastures or hay, and methane emissions were not different from feedlot-fed cattle.