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Plant Pathology

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Investigating Birds As Dispersal Vectors Of Litylenchus Crenatae Subsp. Mccannii (Anguinidae), The Nematode Associated With Beech Leaf Disease, Spencer Rock Parkinson Jan 2024

Investigating Birds As Dispersal Vectors Of Litylenchus Crenatae Subsp. Mccannii (Anguinidae), The Nematode Associated With Beech Leaf Disease, Spencer Rock Parkinson

Graduate Theses, Dissertations, and Problem Reports

Beech leaf disease (BLD) is an emerging forest pathogen primarily affecting American beech (Fagus grandifolia, Ehrh.) in North America and has been attributed to tree mortality of sapling sized trees within five to seven years of infection. Symptoms typically occur in regenerating American beech thickets sprouting from roots of trees killed by beech bark disease. Scientists first observed BLD in Ohio in 2012 and currently has spread to 15 states in the USA and one Canadian province. The nematode Litylenchus crenatae subsp. mccannii (Lcm) is highly associated with BLD symptoms, interveinal chlorosis and defoliation of leaves, and is currently …

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Environmental Biology Masters Capstone, Antonio Gonzalez-Pita Jan 2024

Environmental Biology Masters Capstone, Antonio Gonzalez-Pita

Regis University Student Publications (comprehensive collection)

Human wildlife interactions (HWI) pose a complex challenge for wildlife managers. Human encroachment into wildlife habitat and the growing number of outdoor recreationists are increasing the frequency of contact and conflict, especially in regions such as the Front Range of Colorado. Geographic information systems (GIS), which use a combination of remote sensing and environmental survey data, allow for predictive spatial analyses of where human wildlife interactions are likely to occur. I used publicly reported observations of moose to create spatial predictive maps in a species distribution model framework. Slope and elevation were shown to be the strongest predictors of HWI, …

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Assessing Endophyte Frequency Distributions And The Effect Of Epichloë Brachyelytri In The Chemotypic And Genotypic Diversity Of Brachyelytrum Erectum, Rachel Ann Sneed Jan 2024

Assessing Endophyte Frequency Distributions And The Effect Of Epichloë Brachyelytri In The Chemotypic And Genotypic Diversity Of Brachyelytrum Erectum, Rachel Ann Sneed

Theses and Dissertations--Plant Pathology

Seed-transmissible epichloid fungal endophytes are best known for their roles as defensive mutualists in cool-season grasses. Historically, the discovery of fungal endophytes was driven by investigations of plant toxicity to livestock, followed by extensive study of their alkaloids and protection against insects and nematodes. Epichloae can produce four classes of alkaloids: ergot alkaloids, lolines (saturated aminopyrrolizidines), indole–diterpenes, and peramine. It is increasingly evident that these hereditary symbionts have much more diverse chemical profiles both in individual populations and between them. To this end, differences in chemotypic profiles of these symbionts may translate to different evolutionary and environmental advantages across plant …

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Habitat And Demography Of The Ozark Chinquapin (Castanea Ozarkensis) At Roaring River State Park In Barry County, Missouri, Danielle Evilsizor Dec 2023

Habitat And Demography Of The Ozark Chinquapin (Castanea Ozarkensis) At Roaring River State Park In Barry County, Missouri, Danielle Evilsizor

Electronic Theses & Dissertations

The Ozark chinquapin, Castanea ozarkensis Ashe, is a chestnut tree with a range concentrated in the Interior Highlands of North America. Like other North American members of Castanea, it was reduced from an overstory tree to an understory shrub by the invasive chestnut blight fungus (Cryphonectria parasitica [Murrill] M.E. Barr) during the early 20th century. However, relatively little is known about the habitat of this species or its health and reproductive capability post chestnut blight. Chapter one of this study analyzed the habitat of this species through a random forest species distribution model (SDM) to predict where …

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Characterization Of Lignin Structural Variability And The Associated Application In Genome Wide Association Studies, Nathan D. Bryant Dec 2023

Characterization Of Lignin Structural Variability And The Associated Application In Genome Wide Association Studies, Nathan D. Bryant

Doctoral Dissertations

Poplar (Populus sp.) is a promising biofuel feedstock due to advantageous features such as fast growth, the ability to grow on marginal land, and relatively low lignin content. However, there is tremendous variability associated with the composition of biomass. Understanding this variability, especially in lignin, is crucial to developing and implementing financially viable, integrated biorefineries. Although lignin is typically described as being comprised of three primary monolignols (syringyl, guaiacyl, p-hydroxyphenyl), it is a highly irregular biopolymer that can incorporate non-canonical monolignols. It is also connected by a variety of interunit linkages, adding to its complexity. Secondary cell wall …

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Cellular Control Of Proteostasis, Cytoskeleton Organization, And Polarity Remodeling During Infection-Related Development By The Rice Blast Fungus, Audra Rogers Dec 2023

Cellular Control Of Proteostasis, Cytoskeleton Organization, And Polarity Remodeling During Infection-Related Development By The Rice Blast Fungus, Audra Rogers

Graduate Theses and Dissertations

Understanding the intricate processes underlying fungal infection-related development is crucial for combating plant pathogens. In this study, we investigate the coordination of morphogenetic transitions during plant infection by Magnaporthe oryzae, the blast fungus. We focus on three key aspects: protein quality control, cytoplasmic microtubule organization, and cell-end marker proteins. M. oryzae forms a specialized infection cell known as an appressorium. The proper formation of this cell is crucial for the pathogen to infect a susceptible host. First, we investigate protein quality control mechanisms during infection-related development. Our findings demonstrate that aggregated proteins are sequestered into quality control compartments within conidia …

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Molecular Identification And Characterization Of Heat Shock Protein 70 Family Proteins Essential For Turnip Mosaic Virus Infection In Arabidopsis Thaliana, Ziwei Tang Sep 2023

Molecular Identification And Characterization Of Heat Shock Protein 70 Family Proteins Essential For Turnip Mosaic Virus Infection In Arabidopsis Thaliana, Ziwei Tang

Electronic Thesis and Dissertation Repository

Turnip mosaic virus (TuMV) belongs to the RNA virus family of Potyviridae and genus Potyvirus. TuMV incurs agricultural losses by causing diseases in vegetable, oilseed, forage, and biofuel crops globally. Viruses are obligate intracellular parasites depending on the host cellular machinery to proliferate. Thus, molecular identification and functional characterization of host factors essential in the viral infection process may open up a new avenue towards developing genetic virus resistance. Eukaryotic translation initiation factor 4E (eIF4E) or its isoform (eIF(iso)4E) is a critical host factor for many potyviruses including TuMV. Heat shock protein 70 family proteins (HSP70) have been identified in …

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Development And Use Of A Detached Leaf Assay To Assess Soybean Cultivar Resistance To Target Spot, Caused By Corynespora Cassiicola, Rachael Wolf Aug 2023

Development And Use Of A Detached Leaf Assay To Assess Soybean Cultivar Resistance To Target Spot, Caused By Corynespora Cassiicola, Rachael Wolf

Graduate Theses and Dissertations

Target spot, caused by the fungus Corynespora cassiicola, was once considered a minor foliar disease of soybean in the US. However, a severe outbreak of target spot occurred in the southeastern U.S in 2016. Due to the lack of information on resistance in soybean cultivars, and the challenges in screening cultivars in the field, a detached leaf assay was developed to evaluate resistance. The objectives of the effort were to 1) develop a greenhouse inoculation method of soybean with C. cassiicola 2) use that method to compare reactions of soybean germplasm lines to target spot 3) collect and characterize isolates …

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Evaluating The Effect Of Biochar Soil Amendments On Belonolaimus Longicaudatus Populations Damaging Bermudagrass In South Carolina, Malone Thomason Aug 2023

Evaluating The Effect Of Biochar Soil Amendments On Belonolaimus Longicaudatus Populations Damaging Bermudagrass In South Carolina, Malone Thomason

All Theses

Belonolaimus longicaudatus Rau, (1958) (sting nematode) is a prevalent plant-parasitic nematode found in association with Cynodon spp. (bermudagrass) in South Carolina (Ye et al., 2012). Due to the persistence of the pest, long-term management strategies are necessary. Applications of biochar and compost amendments have reduced nematodes in various crop systems, but research on turfgrass systems is limited. The objectives of the studies were to determine: (1) The effect of topdressing or soil incorporated biochar amendments on sting nematode populations, and (2) The effect of topdressing or soil incorporated amendments on turf quality. A field study was established to examine topdressings …

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Use Of Biorational Products For Botrytis Management In Floriculture Crops, Josselyn Calidonio Aug 2023

Use Of Biorational Products For Botrytis Management In Floriculture Crops, Josselyn Calidonio

All Theses

Botrytis cinerea is an important pathogen that has a significant economic impact on the floriculture industry from propagation to the postharvest environment. Chemical fungicide applications have been one of the main approaches that growers use for botrytis blight management; however, studies have shown that the indiscriminative use of these chemicals leads to fungicide resistance (Brent and Hollomon, 1998; Fillinger and Elad 2016). This thesis explores the potential use of biorational products for botrytis blight management. Biorationals are defined as compounds that have low or no direct mammalian toxicity and few effects on the environment (Paulitz and Belanger 2001). A review …

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Screening For Dmi And Mbc Fungicide Resistance In Monilinia Fructicola And Evaluation Of Biorational Products For Control Of Brown Rot On Peach In The Southeast United States, William Gura Aug 2023

Screening For Dmi And Mbc Fungicide Resistance In Monilinia Fructicola And Evaluation Of Biorational Products For Control Of Brown Rot On Peach In The Southeast United States, William Gura

All Theses

Monilinia fructicola (G. Winter) Honey is a fungal pathogen and is the causal agent of blossom blight, twig blight, green fruit rot, preharvest brown rot, and postharvest brown rot of peach (Prunus persica (L.) Batsch). Especially pre-and postharvest brown rot can have devastating economic impacts and negatively effects yield for peach growers throughout the southeastern United States. The most effective method for the control of pre- and postharvest brown rot is the application of synthetic fungicides during preharvest season. However, the consecutive use of fungicides with the same mode of action potentially give rise to resistance. This thesis focuses …

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Fungal Plant Pathogens Affecting Crops In The Lower Rio Grande Valley, Isabel Martinez Aug 2023

Fungal Plant Pathogens Affecting Crops In The Lower Rio Grande Valley, Isabel Martinez

Theses and Dissertations

The Lower Rio Grande Valley (LRGV) located in South Texas has a subtropical and humid environment which is conducive for plant disease. In Chapter 1, Southern blight caused by Athelia rolfsii was collected from Edinburg, Texas in tomato and bean crops. The present study focused on assessing the role of temperature on growth and sclerotia production. We found that there is an optimal growing temperature for both hyphal growth and sclerotia production. In Chapter 2, red rot fungal disease was collected from sugarcane in Texas. Morphology, pathogenicity, effects of temperature, and biocontrol agents were assessed. Isolate morphology closely resembled Fusarium …

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Molecular Identification Of Oomycete Species Associated With Woody Plants In Louisiana And Survey Of Oomycete Species Associated With Live Oak Trees Planted On The Louisiana State University Campus, Hamilton Crockett Jul 2023

Molecular Identification Of Oomycete Species Associated With Woody Plants In Louisiana And Survey Of Oomycete Species Associated With Live Oak Trees Planted On The Louisiana State University Campus, Hamilton Crockett

LSU Master's Theses

Three Oomycetes genera, Phytophthora, Phytopythium and Pythium, contain many important plant pathogens. Historically, Phytophthora infestans, responsible for the great Irish potato famine, and Phytophthora ramorum, causal agent of sudden oak death disease, are the two better-known high impact pathogens. The LSU AgCenter Plant Diagnostic Center (PDC) has been isolating putative Phytophthora and related species from combined root and soil samples collected from various woody ornamentals and trees for over a decade. In August of 2016, Louisiana received torrential rains, which resulted in historic flooding in several southern parishes. During subsequent years, the PDC staff isolated a …

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Factors Affecting Apothecia Production And Primary Infection By Monilinia Vaccinii-Corymbosi On Vaccinium Angustifolium, Ian Leonard May 2023

Factors Affecting Apothecia Production And Primary Infection By Monilinia Vaccinii-Corymbosi On Vaccinium Angustifolium, Ian Leonard

Electronic Theses and Dissertations

Mummy berry, caused by Monilinia vaccinii-corymbosi (MVC), is a prolific disease of Vaccinium angustifolium (wild blueberry) leading to decreased yield in wild blueberry fields throughout the Downeast (DE) and Midcoast (MC) regions of Maine (ME). This study aimed to identify factors affecting primary inoculum production and infection by MVC on wild blueberry, and what bud stages of wild blueberry are most susceptible to infection. Through common garden (CGE), field and incubation experiments conducted in 2021 and 2022, factors affecting carpogenic germination of MVC pseudosclerotia and relationships between susceptible wild blueberry buds and environmental factors were analyzed. The CGE conducted in …

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Epidemiological Approaches For Practical Solutions To Pertinent Peanut Problems, Hope Becton May 2023

Epidemiological Approaches For Practical Solutions To Pertinent Peanut Problems, Hope Becton

All Dissertations

Peanut is an important food crop grown in the southeastern region of the United States. Diseases are the most yield-limiting component in peanut production with one of the most important being late leaf spot (LLS), caused by Nothopassalora personata (Np). The disease initially appears in the lower canopy as dark brown to black lesions and conidiophores bearing conidia are produced on the abaxial side of the lesion. Np is passively dispersed primarily by wind and rain events. Spore traps were used to detect and quantify conidia captured up to 70 m from an inoculum source and to further assess …

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A Small Stem Assay Using Hypovirulent Cryphonectria Parasitica To Screen Castanea Dentata Backcross F2 Families May Set The Stage For Long-Term Survival, Trish Nguyen May 2023

A Small Stem Assay Using Hypovirulent Cryphonectria Parasitica To Screen Castanea Dentata Backcross F2 Families May Set The Stage For Long-Term Survival, Trish Nguyen

Honors Theses

By combining the results of blight resistance breeding and the application of hypovirulence as a biocontrol, populations of resistant hybrid trees could be deployed together with a less pathogenic strain of Cryphonectria parasitica (Murr.) Barr. I used a small stem assay to screen seedlings in thirteen half-sibling backcross F2 families with an attenuated strain of C. parasitica containing the Cryphonectria parasitica hypovirus-1 (CHV-1) Euro7 virus. The experiment was set up as a randomized complete block design in 2-gallon containers. Measurements of canker length and morphology were gathered at 90 days post-inoculation. Although statistically significant differences were seen between canker lengths …

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Role Of Kinases And An Epigenetically Regulated Gene In Soybean Cyst Nematode Resistance, Tracy Elizabeth Hawk May 2023

Role Of Kinases And An Epigenetically Regulated Gene In Soybean Cyst Nematode Resistance, Tracy Elizabeth Hawk

Doctoral Dissertations

Soybean cyst nematode (SCN) is the most devastating and economically important pathogen of soybean across the United States and globally. The current primary control for SCN is with the use of resistant cultivars, however, SCN populations are shifting to overcome most common sources of soybean resistance. This research was conducted to identify novel sources of genetic resistance to SCN. First, I functionally characterized two protein kinase hub genes whose function are predicted to be essential for mediating soybean susceptibility to SCN. I generated kinase-dead variants by mutating two amino acids required for the kinase enzymatic activity. Overexpression of the kinase-dead …

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Oxalic Acid Leaf Disk Soak Assay Is A New Possibility In Screening For Blight Resistance In Castanea Species, Kaitlyn Harden May 2023

Oxalic Acid Leaf Disk Soak Assay Is A New Possibility In Screening For Blight Resistance In Castanea Species, Kaitlyn Harden

Honors Theses

Chestnut blight is a disease caused by the ascomycete fungus Cryphonectria parasitica in the Castanea species. The fungus uses oxalic acid (OA) to attack the tree’s cells. Castanea dentata, the American chestnut, was wiped out by chestnut blight in the early to mid-20th century, but several East Asian Castanea species appear highly resistant to the fungus. To breed resistant American type trees, screening methods are used to enable selection of interspecific hybrids. The alternative small stem assay (aSSA) is a method of screening container-grown hybrid seedlings during their first growing season by directly infecting them with C. parasitica. Some plant …

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Laser Inscribed Graphene Aptasensor For Detection Of Apple Stem Pitting Virus, Yifan Tang May 2023

Laser Inscribed Graphene Aptasensor For Detection Of Apple Stem Pitting Virus, Yifan Tang

All Theses

Apple stem pitting virus (ASPV) is a latent RNA virus that could cause significant loss to apple production; early-stage field diagnosis could reduce the economic loss caused by ASPV infection. Biosensor is a promising user-friendly detection tool that could transduce biological or chemical interactions into electric signals. Among various types of biosensors, aptasensor, which use aptamers as recognition elements show potential as a high throughput, in-field test. The goal of this thesis is to develop an electrochemical aptasensor testing system for ASPV detection. The quality control study of laser inscribed graphene (LIG) based working electrode of the biosensor was conducted …

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Fungicide Sensitivity, Race Structure, And Host Pathogenicity Of Phytophthora Nicotianae In Tennessee And North Carolina, Taylor Giovanna Miller May 2023

Fungicide Sensitivity, Race Structure, And Host Pathogenicity Of Phytophthora Nicotianae In Tennessee And North Carolina, Taylor Giovanna Miller

Masters Theses

Phytophthora nicotianae, a hemibiotrophic oomycete, is the causal agent of disease of over 300 hosts, notably tobacco, tomato, citrus, strawberry, and ornamentals. Black shank of tobacco is a devastating disease that can result in up to 100% yield loss if left untreated, and buckeye rot of tomato compromises the marketability and yield of fruit impacted by P. nicotianae. Black shank and buckeye rot have been reported throughout the state of Tennessee, and the goal of this work was to analyze fungicide sensitivity of P. nicotianae isolated from tobacco and tomato, characterize the race structure of P. nicotianae, …

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Management Of Nematodes Associated With Tobacco And Sweet Potato Using Non-Fumigant Approach, Md Shah Alam May 2023

Management Of Nematodes Associated With Tobacco And Sweet Potato Using Non-Fumigant Approach, Md Shah Alam

All Theses

Tobacco and sweet potato are attacked by several species of plant-parasitic nematodes leading to substantial yield losses. Because host-plant resistance is lacking in commercial cultivars and other common methods of nematode management either have low efficacy or pose adverse effects to the environment, controlled environment studies were conducted to evaluate the efficacy of non-fumigant chemical and biological nematicides against Meloidogyne enterolobii on tobacco and Rotylenchulus reniformis on sweet potato. Results suggest that fluensulfone was the most effective in suppressing M. enterolobii in tobacco, followed by oxamyl and fluopyram. The biological Burkholderia derived biological nematicide did not suppress the nematode population. …

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Exploring The Effects Of Environmental Factors On Rice Blast Disease, April Ellen Lamb Jan 2023

Exploring The Effects Of Environmental Factors On Rice Blast Disease, April Ellen Lamb

Theses and Dissertations--Plant Pathology

Rice blast disease, caused by the ascomycete fungus Magnaporthe oryzae, occurs in over 85 countries and results in an annual crop loss of 10-30%, a corresponding nutrient value of meals for 60 million people. As a result, it is listed as a critical plant disease by the United Nations. Understanding factors affecting disease severity is of critical concern for food security. M. oryzae has been used as a model system for studying effector-triggered immunity (ETI) by understanding that ETI is primarily a plant response. M. oryzae has been used as a model to study fungal pathogenicity, host specificity, genome …

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Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir Jan 2023

Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir

Electronic Theses and Dissertations

Pyraclostrobin, a QoI fungicide, is extensively used in the management of fungal leaf spot diseases. In this study, 215 P. tritici-repentis isolates collected from wheat fields in South Dakota were evaluated for their sensitivity to Pyraclostrobin. Of 215 isolates, 48 isolates (22%) exhibited insensitivity based on G143A mutation and EC50 values using spore germination assay. Our results indicate reduced sensitivity to insensitivity to pyraclostrobin in some isolates suggesting regular monitoring of the P. tritici-repentis population to QoI fungicides is essential to track the evolution of insensitive population. Spelt wheat (Triticum spelta L.) is an ancient cereal that is gaining small …

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Unveiling Race Diversity And Fungicide Sensitivity Profiles Of Pyrenophora Tritici Repentis Populations In South Dakota Collected From 2021-2023, Jaswinder Kaur Jan 2023

Unveiling Race Diversity And Fungicide Sensitivity Profiles Of Pyrenophora Tritici Repentis Populations In South Dakota Collected From 2021-2023, Jaswinder Kaur

Electronic Theses and Dissertations

Tan spot, a foliar disease of wheat caused by the ascomycetes fungus Pyrenophora tritici repentis (Ptr), poses a significant threat to wheat crops globally. The disease gained economic importance in the late 1970s due to shifts in farming practices, such as minimal tillage and planting on crop residues, providing favorable conditions for the pathogen to overwinter and cause infections. Ptr is classified into eight races based on the production of necrotrophic effectors—Ptr ToxA (necrosis-inducing), Ptr ToxB, and Ptr ToxC (chlorosis-inducing on respective susceptible and sensitive cultivars)—resulting in distinct symptoms on susceptible wheat differentials. The inverse gene-for-gene hypothesis of Ptr suggests …

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Fusarium Head Blight Resistance Assessment In Barley, Tasneem Fathima Jan 2023

Fusarium Head Blight Resistance Assessment In Barley, Tasneem Fathima

Electronic Theses and Dissertations

Fusarium head blight (FHB), commonly called scab is the most devastating disease of wheat and barley caused by the ascomycete fungus, Fusarium graminearum. FHB affects both the quality and quantity of cereal grains affecting the supply chain of the food and feed industry and the marketability of the produce. Barley (Hordeum vulgare L.) is among the several crop species most vulnerable to FHB infection. An estimated 25% of Spring barley grain production in the United States is used to make malted beverages such as beer. Malting barley is a relatively high-value crop with high demands regarding grain quality. FHB directly …

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The Effects Of Residue Amounts And Variety Selection On The Development Of Wheat Fungal Pathogens In South Dakota, Abrielle Tembreull Jan 2023

The Effects Of Residue Amounts And Variety Selection On The Development Of Wheat Fungal Pathogens In South Dakota, Abrielle Tembreull

Electronic Theses and Dissertations

Roughly 50% of South Dakota cropland is under the practice of no-till, with large increases in acres of no-till in the last 20 or so years. No-till is beneficial for Great Plains states, such as South Dakota, as soil residues are withheld. Residues are shown to increase soil health through retention of moisture and increase of microorganisms, but residues can serve as a source of inoculum for fungal pathogens that can overwinter on them. As wheat is one of the most important food crops in the world, it is important to protect this vital crop from diseases. Diseases of wheat …

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Biological Control Of Bacterial Leaf Streak Of Wheat Using Antagonistic Rhizobacteria, Ashley Frederickson Jan 2023

Biological Control Of Bacterial Leaf Streak Of Wheat Using Antagonistic Rhizobacteria, Ashley Frederickson

Electronic Theses and Dissertations

Wheat (Triticum aestivum L.) is one of the world’s most valuable crops, and the primary food grain produced in the United States. A major disease in wheat production is Bacterial Leaf Streak (BLS) caused by the gram-negative bacterium Xanthomonas translucens pv. undulosa (Xtu). Very few management strategies are available to growers to manage this disease, as common chemicals do not provide adequate control. A number of studies have been conducted on biological control using Rhizospheric bacteria for the control of various plant diseases, but limited data is available on the efficacy of biological control using Rhizospheric bacteria for controlling Bacterial …

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Natural Transmission Of Grapevine Vein Clearing Virus, Matthew Manu Jan 2023

Natural Transmission Of Grapevine Vein Clearing Virus, Matthew Manu

MSU Graduate Theses

More than 60 million tons of grapes are produced annually in the world, making them one of the most widely grown fruit crops. Despite grapes’ economic and health benefits, biotic stressors, such as viruses, cause significant loss to the grape and wine industry. One such virus is grapevine vein clearing virus (GVCV) which seriously threatens grape cultivation in the Midwest region of the United States. This virus has caused the removal of seven commercial vineyards since its discovery in 2004. About 34% of Ampelopsis cordata wild vines are infected with GVCV and serve as a primary inoculum for the spread …

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Distribution Patterns Of Allorhizobium Vitis In Missouri Vineyards And Non-Vineyard Soils, Jacquelyn M. Wray Jan 2023

Distribution Patterns Of Allorhizobium Vitis In Missouri Vineyards And Non-Vineyard Soils, Jacquelyn M. Wray

MSU Graduate Theses

Crown gall disease causes significant economic loss to the grape and wine industry. Preventive strategies are most effective for mitigating the loss of grapevines in vineyards, as there is no known cure for this disease. The bacterium Allorhizobium vitis carrying a tumor-inducing (Ti) plasmid is the causative agent. A. vitis bacteria live systemically in the grapevine before causing visible symptoms and can survive in residual plant tissues and soil for more than two years. Diagnostic methods have been developed to detect A. vitis bacteria in grapevines and soil. However, more reliable, specific, and high-throughput diagnostics are still needed for screening …

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Investigation Of Fungal Pathogens And Woodboring Beetles Of Sugarbush Stands In The Central Appalachian Region, Molly A. Sherlock Jan 2023

Investigation Of Fungal Pathogens And Woodboring Beetles Of Sugarbush Stands In The Central Appalachian Region, Molly A. Sherlock

Graduate Theses, Dissertations, and Problem Reports

The production of maple syrup is a valuable source of income and cultural identity for many rural communities throughout the central Appalachian region. Regardless, maple syrup producers face difficulties maintaining the health and productivity
of their stands due to various impacts of climate change, which have given rise to extreme weather events that exacerbate damage caused by pests and diseases. To ensure the long-term sustainability of the maple syrup industry, it is crucial to obtain a comprehensive understanding of the current biotic threats within the region, particularly woodboring insects and fungal pathogens. However, insufficient information exists regarding the community assemblages …

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