Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Deletion Of The Δ12-Oleic Acid Desaturase Gene Of A Nonaflatoxigenic Aspergillus Parasiticus Field Isolate Affects Conidiation And Sclerotial Development, Perng Kuang Chang, Richard A. Wilson, Nancy P. Keller, Thomas E. Cleveland
Deletion Of The Δ12-Oleic Acid Desaturase Gene Of A Nonaflatoxigenic Aspergillus Parasiticus Field Isolate Affects Conidiation And Sclerotial Development, Perng Kuang Chang, Richard A. Wilson, Nancy P. Keller, Thomas E. Cleveland
Fungal Molecular Plant-Microbe Interactions
Aims: To investigate how linoleic acid affects conidial production and sclerotial development in a strictly mitotic Aspergillus parasiticus field isolate as related to improving biocompetitivity of atoxigenic Aspergillus species.
Methods and Results: We disrupted A. parasiticus Δ12-oleic acid desaturase gene (odeA) responsible for the conversion of oleic acid to linoleic acid. We examined conidiation and sclerotial development of SRRC 2043 and three isogenic mutant strains deleted for the odeA gene (DodeA), either with or without supplementing linoleic acid, on one complex potato dextrose agar (PDA) medium and on two defined media: nitrate-containing Czapek agar (CZ) and Cove’s ammonium …