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Life Sciences Commons

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Plant Breeding and Genetics

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

2016

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Evaluation Of Harvest Time/Temperature And Storage Temperature On Postharvest Incidence Of Red Drupelet Reversion Development And Firmness Of Blackberry (Rubus L. Subgenus Rubus Watson), Jack E. Mccoy, John R. Clarke, Alejandra A. Salgado, Andrew Jecmen Jan 2016

Evaluation Of Harvest Time/Temperature And Storage Temperature On Postharvest Incidence Of Red Drupelet Reversion Development And Firmness Of Blackberry (Rubus L. Subgenus Rubus Watson), Jack E. Mccoy, John R. Clarke, Alejandra A. Salgado, Andrew Jecmen

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Since 1964, the University of Arkansas blackberry breeding program has worked to improve fruit quality and shipping capabilities. A major limitation in blackberry fruit is postharvest handling potential for the shipping market. Maintaining fruit firmness in storage is crucial. Red drupelet reversion (or simply reversion) is also an important postharvest disorder in which drupelets change from black to red during storage. It is hypothesized that reversion is increased when fruit is picked at hot temperatures and exposed to a rapid change of temperature. These studies evaluated harvest time/temperature, as well as storage temperature, on berry firmness and the incidence of …


Efficiency Of The Nuclease I-Scei In Excising Selectable Marker Genes From The Plant Genome, Elliot E. Pruett, Soumen Nandy, Vibha Srivastava Jan 2016

Efficiency Of The Nuclease I-Scei In Excising Selectable Marker Genes From The Plant Genome, Elliot E. Pruett, Soumen Nandy, Vibha Srivastava

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Gene stacking is a method used in biotechnology by which multiple genes can be placed at a single genomic site, thereby simplifying plant breeding. In this approach, DNA nucleases are used for excising selectable marker genes (SMG), which are the unneeded components of transgenic plants. The goal of this project is to evaluate the effectiveness of the nuclease I-SceI in excising DNA in plants. Specifically, this study tests heat-inducible I-SceI through the use of a heat-shock promoter (HS) in order to control SMG excision by heat application. The DNA plasmid containing a visual marker gene flanked by I-SceI target sites …