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Plant Breeding and Genetics

University of the Philippines Los Baños

2021

Articles 1 - 8 of 8

Full-Text Articles in Life Sciences

First Detection Of Naturally Introgressed Bt Cry1ab In Asian Corn Borer [Ostrinia Furnacalis (Guenée)] - Resistant Traditional Maize (Silangan) In The Philippines, Merdelyn T. Caasi-Lit, Eureka Teresa C. Ocampo, Artemio Salazar, Jefferson Paril, Bryan Novio, Bernard Panabang, Angelyn Marta Marmeto Dec 2021

First Detection Of Naturally Introgressed Bt Cry1ab In Asian Corn Borer [Ostrinia Furnacalis (Guenée)] - Resistant Traditional Maize (Silangan) In The Philippines, Merdelyn T. Caasi-Lit, Eureka Teresa C. Ocampo, Artemio Salazar, Jefferson Paril, Bryan Novio, Bernard Panabang, Angelyn Marta Marmeto

The Philippine Agricultural Scientist

Resistance against insect pests is one of the most promising and highly favored characteristics of an economically important crop. In the Philippines, the Asian corn borer (ACB), Ostrinia furnacalis (Guenée), is the most destructive insect pest of maize. The discovery of Bacillus thuringiensis and the Bt toxin, which led to the development of Bt corn, effectively controlled this pest. In this study, four traditional varieties of maize that showed resistance against ACB were tested for the presence of Bt Cry1Ab protein. Out of these four, a traditional variety from San Carlos City, Pangasinan, which was initially thought to have natural …

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Establishment Of The In Vitro Culture And Plant Regeneration Of Anubias Barteri Var. Nana 'Mini', Kuan-Hung Lin, Chih-Peng Kuo, Li-Ru Chen Dec 2021

Establishment Of The In Vitro Culture And Plant Regeneration Of Anubias Barteri Var. Nana 'Mini', Kuan-Hung Lin, Chih-Peng Kuo, Li-Ru Chen

The Philippine Agricultural Scientist

Anubias barteri is an ornamental aquatic plant of economic importance worldwide. Effective removal of contaminants has been a major problem for the in vitro propagation of A. barteri. Hydrogen peroxide (H2O2) was applied as a pre-disinfectant to treat rhizome bud explants of A. barteri var. nana ‘Mini’ followed by disinfection with mercuric chloride (HgCl2) to eliminate in vitro contamination. However, application of 0.1% HgCl2 for 5 min without pre-disinfecting with H2O2 significantly reduced the contamination rate to 44.4% compared to other combinations. Moreover, in vitro shoots of A. barteri …

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Epinasty Accompanies Potassium Nitrate-Induced Flower Bud Break In 'Carabao' Mango [Research Note], Ulysses Besas, Calixto Protacio Dec 2021

Epinasty Accompanies Potassium Nitrate-Induced Flower Bud Break In 'Carabao' Mango [Research Note], Ulysses Besas, Calixto Protacio

The Philippine Agricultural Scientist

The hypothesis that potassium nitrate application promotes epinasty in ‘Carabao’ mango through increased ethylene production was investigated. The degree of drooping, apical primordium growth as affected by shoot ethylene and levels after potassium nitrate spraying were monitored. Evidence presented established for the first time in ‘Carabao’ mango that epinasty occurred just before flower emergence, coinciding with visible bud break, and extended up to 18 days, marking floral anthesis. The possible reasons could be the higher levels of ethylene at 0.31% and earlier increase of ethylene in the shoot apex at four hours and apical primordium growth. Increased ethylene levels at …

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Genetic Diversity And Population Structure Of Sugarcane (Saccharum Officinarum L.) Collected From Seven Philippine Regions For Use As Breeding Stocks For Crop Improvement, Jan Leandro Acedera, Jhun Laurence Rasco, Jemimah Banganan, Antonio Lalusin Sep 2021

Genetic Diversity And Population Structure Of Sugarcane (Saccharum Officinarum L.) Collected From Seven Philippine Regions For Use As Breeding Stocks For Crop Improvement, Jan Leandro Acedera, Jhun Laurence Rasco, Jemimah Banganan, Antonio Lalusin

The Philippine Agricultural Scientist

Selection of parents for hybridization relies on the information on their genetic relationship and diversity which are essential in any breeding program. This study aimed to estimate the extent of genetic diversity and population structure of 76 sugarcane accessions from seven regions in the Philippines using 57 morphological characters and 50 microsatellite markers. The sugarcane collections exhibited moderate to high diversity with mean of H’ = 0.72 for qualitative and H’ = 0.75 for quantitative morphological characters, respectively. This is corroborated by the analysis of variance (ANOVA) of agronomic parameters, except for stalk length. Unweighted Pair Group Method with Arithmetic …

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Spectral Lights Based Treatment Enhanced Biomass Accumulation And Secondary Metabolites Production In Callus Culture Of Citrus Reticulata, Faiza Anum, Naveed Iqbal Raja, Tahira Sultana, Abeer Kazmi, Amir Ali, Bushra Qayyum, Anza Afzal, Aneela Nijibat, Zia-Ur-Rehman Mashwani, Abeer Kazmi Sep 2021

Spectral Lights Based Treatment Enhanced Biomass Accumulation And Secondary Metabolites Production In Callus Culture Of Citrus Reticulata, Faiza Anum, Naveed Iqbal Raja, Tahira Sultana, Abeer Kazmi, Amir Ali, Bushra Qayyum, Anza Afzal, Aneela Nijibat, Zia-Ur-Rehman Mashwani, Abeer Kazmi

The Philippine Agricultural Scientist

Citrus reticulata is a common fruit, belongs to the family Rutaceae. It contains terpenes, phenolics, flavonoids, and vitamin C in conjugation with sugars, amino acids, minerals, organic acids and other biologically important compounds. Application of light elicitation strategies in in vitro plant cultures is acquiring more interest for their potential in feasible biomass accumulation and therapeutically important secondary metabolites production. The present study was designed to investigate the effects of different monochromatic lights (white, red, blue, green, and yellow) on the proliferation and secondary metabolites accumulation in callus cultures of Citrus reticulata raised in response to treatment with AgNPs and …

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Efficacy Study Of Selected Maize (Zea Mays L.) Genotypes Against The Asian Corn Borer, Ostrinia Furnacalis (Guenée), Yoryncita Marfori, Merdelyn Caasi-Lit, Tonette Laude Sep 2021

Efficacy Study Of Selected Maize (Zea Mays L.) Genotypes Against The Asian Corn Borer, Ostrinia Furnacalis (Guenée), Yoryncita Marfori, Merdelyn Caasi-Lit, Tonette Laude

The Philippine Agricultural Scientist

Laboratory and screen house studies were conducted to assess the efficacy of selected twenty-six maize varieties against Asian corn borer (ACB), Ostrinia furnacalis (Guenée). Hierarchical tree based on resistance parameters clearly revealed that the twenty-six maize varieties are best classified into three groups, viz. resistant, intermediate, and susceptible. In laboratory test, the varieties RCS2, RCS4, RCS5, 'IPB Var13', Bt2, Bt3, and Bt4 were consistently identified as highly resistant. Further examinations in stalk feeding assay showed that the resistant varieties had sharp decline in larval survival (LS) on the 2nd to the 5th day of larval feeding in …

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Weedy Rice Conserved Ex Situ Characterized Using Morphological And Simple Sequence Repeat (Ssr) Markers, Rubylyn Mijan Infante, Maria Elizabeth B. Naredo, Merlyn S. Mendioro Jun 2021

Weedy Rice Conserved Ex Situ Characterized Using Morphological And Simple Sequence Repeat (Ssr) Markers, Rubylyn Mijan Infante, Maria Elizabeth B. Naredo, Merlyn S. Mendioro

The Philippine Agricultural Scientist

A clear understanding of genetic diversity in weedy rice is important in improving protocols for its conservation, ex situ. To this end, Asian collections of weedy rice together with accessions of the wild AA genome species Oryza nivara Sharma et Shastry and Oryza rufipogon Griffith, and the cultivated species, Oryza sativa L. were characterized using 16 qualitative morphological traits and 18 SSR markers. The Shannon-Weaver index (H’) revealed higher diversity in the weedy (mean H’ = 0.76) and wild O. nivara (mean H’ = 0.79) and O. rufipogon samples (mean H’ = 0.69) compared to the cultivated …

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Grain Yield Variations In Rice Genotypes Under Different Growing Environments In The Philippines, Kim Nyka C. Perdiguerra, Shiro Mitsuya, Akira Yamauchi, Felino P. Lansigan, Maria Victoria O. Espaldon, Jose E. Hernandez, Pompe C. Sta. Cruz Jun 2021

Grain Yield Variations In Rice Genotypes Under Different Growing Environments In The Philippines, Kim Nyka C. Perdiguerra, Shiro Mitsuya, Akira Yamauchi, Felino P. Lansigan, Maria Victoria O. Espaldon, Jose E. Hernandez, Pompe C. Sta. Cruz

The Philippine Agricultural Scientist

Grain yield variations in PSB Rc18, NSIC Rc222 (inbreds) and NSIC Rc202H (hybrid) were determined across growing environments as a function of cropping seasons and locations in rice producing areas in the Philippines. Contribution of location to variation in grain yield is 61.0%, while 12.7% for the season, and 6.1% for the genotype and this must be due location by season by genotype interactions. Dry season cropping in Nueva Ecija produced the highest mean grain yield. On the other hand, wet season cropping in Davao del Sur produced the least mean grain yield. The genotypes differed in their response to …

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