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Full-Text Articles in Life Sciences

Genome Wide Association Study On Kernel Size In Avena Sativa (Oats), Koryne Ann Carlson Jan 2021

Genome Wide Association Study On Kernel Size In Avena Sativa (Oats), Koryne Ann Carlson

Electronic Theses and Dissertations

Kernel size is a vital milling characteristic for Avena sativa L. (oats), with larger kernel size being desirable for milling facilities. This project evaluated the effect of genotype, environment, and their genotype by environment interaction (G by E) on kernel width, length, and area for a set of 27 oat cultivars grown at multiple locations in South Dakota over three years. Findings indicated significant differences among oat varieties, and that environment had a substantial effect on kernel size. However, we did not see a strong influence of genotype by environment interaction on kernel size. The lack of impact for genotype …


Studies On Genetic Characterization Of Agronomic Traits And Disease Resistance In Bread Wheat, Jyotirmoy Halder Jan 2021

Studies On Genetic Characterization Of Agronomic Traits And Disease Resistance In Bread Wheat, Jyotirmoy Halder

Electronic Theses and Dissertations

A steady increase in wheat yield is vital to feed the continuously rising world population. Systematic exploitation of wheat germplasm and a better understanding of the underlying genetic control could be pivotal in accelerating the genetic gain for yield and disease management. Various modern techniques such as genome-wide association study (GWAS), genomic selection (GS), fine mapping, and cloning can expedite wheat improvement and broaden our understanding of the complex wheat genome. In the first objective of this study, we evaluated the Watkins core set of 121 landrace cultivars (LCs) to identify novel sources of resistance against the tan spot, Stagonospora …


Genome-Wide Association Analysis Permits Characterization Of Stagonospora Nodorum Blotch (Snb) Resistance In Hard Winter Wheat, Rami Altameemi Jan 2021

Genome-Wide Association Analysis Permits Characterization Of Stagonospora Nodorum Blotch (Snb) Resistance In Hard Winter Wheat, Rami Altameemi

Electronic Theses and Dissertations

Stagonospora nodorum blotch (SNB) is an economically important wheat disease caused by the necrotrophic fungus Parastagonospora nodorum. SNB resistance in wheat is controlled by several quantitative trait loci (QTLs). Thus, the identifying of novel resistance/susceptibility QTLs is crucial for continuous improvement of the SNB resistance. Here, the hard winter wheat association mapping panel (HWWAMP) comprising accessions from breeding programs in the Great Plains region of the US, was evaluated for SNB resistance and necrotrophic effectors (NEs) sensitivity at the seedling stage. A genome-wide association study (GWAS) was performed to identify singlenucleotide polymorphism (SNP) markers associated with SNB resistance and …


Exploring Quantitative Trait Loci Mapping For Bud Fruitfulness And Bud Break Traits In Grapevine F2 Population, Seyma Bozkus Jan 2021

Exploring Quantitative Trait Loci Mapping For Bud Fruitfulness And Bud Break Traits In Grapevine F2 Population, Seyma Bozkus

Electronic Theses and Dissertations

Grapevine (Vitis vinifera L. subsp. vinifera) is one of the most important and valuable fruit crops around the world and grape industries in the USA have been growing increasingly regarding the demand for highly productive and quality grapes that can be grown in the cold region around the USA. The development of new cultivars with these features is performed through grape breeding with the help of quantitative trait loci (QTL) mapping, marker-assisted selection (MAS), and and other technologies. In this study, we evaluated grapevine bud fruitfulness by position and bud break in controlled conditions. These traits are multi-genetic and understanding …


Identification Of Loci Influencing Teosinte Crossing Barrier 1 (Tcb1) Efficacy In Maize By Quantitative Trait Loci (Qtl) Mapping And Genome-Wide Association Study (Gwas), Namrata Maharjan Jan 2021

Identification Of Loci Influencing Teosinte Crossing Barrier 1 (Tcb1) Efficacy In Maize By Quantitative Trait Loci (Qtl) Mapping And Genome-Wide Association Study (Gwas), Namrata Maharjan

Electronic Theses and Dissertations

Pollen cross-contamination has been a major problem for maize breeders. Various mechanical methods applied to avoid the contaminations are ineffective. The genetic factors related to maize fertilization can be used to develop an effective method to prevent pollen contamination. Pollen rejection ability controlled by Teosinte crossing barrier 1 (Tcb1) is such a genetic system. Silks possessing dominant Tcb1-s reject pollen possessing the recessive allele (tcb1). Successful fertilization occurs when Tcb1-s pollen falls upon tcb1 silks. The efficacy of dominant Tcb1-s was, however, reduced when repeatedly backcross with maize inbred lines, which suggests that there are modifiers to Tcb1-s. To find …


A Biocontrol Pesticide Derived From Mycovirus-Infected Sclerotinia Sclerotiorum Can Induce Plant Resistance, Connor Pedersen Jan 2021

A Biocontrol Pesticide Derived From Mycovirus-Infected Sclerotinia Sclerotiorum Can Induce Plant Resistance, Connor Pedersen

Electronic Theses and Dissertations

Soybean leaf-associated gemycircularvirus-1 (SlaGemV-1) is a novel mycovirus discovered through the metagenomic sequencing of soybean leaves which is capable of inducing hypovirulence in the highly pathogenic fungus Sclerotinia sclerotiorum. RNASeq analysis techniques were used to determine the transcriptional changes caused by the infection of virus in S. sclerotiorum, as well as the transcriptional changes in Glycine max caused by the colonization of hypovirulent, SlaGemV-1-infected S. sclerotiorum. RNASeq results indicate that viral infection leading to hypovirulence may attenuate expression of genes relating to cell wall synthesis, microtubule formation, and metabolism of steroids and natural antibiotics. Cytochrome P450-related genes, kinesin domain genes, …