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Plant Breeding and Genetics

Old Dominion University

1976

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An Investigation Of The Nucleotide Cyclic 3'5' Adenosine Monophosphate In Young Hypocotyls Of Pisum Sativum L., Gary Marsh Hilton Jul 1976

An Investigation Of The Nucleotide Cyclic 3'5' Adenosine Monophosphate In Young Hypocotyls Of Pisum Sativum L., Gary Marsh Hilton

Biological Sciences Theses & Dissertations

Concentrations of cAMP increased with time in pea hypocotyls assayed at 24 hour intervals over a period of four days. The highest concentration was 20.1 pmoles/mg protein which occurred in 96 hour hypocotyl tips. Ninety-six hour root bodies (dissected region between tip and embryo) achieved a concentration of 8.6 pmoles/mg. Concentrations of cAMP in embryos remained constant around one pmole/mg for three days and values doubled on the fourth day.

Hypocotyls, after germination for 72 hours, were dissected and incubated for various lengths of time in water and 3xl0-8M IAA. Tips incubated for two hours showed a tenfold …