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- Doctoral Dissertations (3)
- Department of Agronomy and Horticulture: Faculty Publications (2)
- Historical Research Bulletins of the Nebraska Agricultural Experiment Station (2)
- Biology Faculty Publications & Presentations (1)
- Chancellor’s Honors Program Projects (1)
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- Dartmouth Scholarship (1)
- Electronic Theses and Dissertations (1)
- Graduate Theses and Dissertations (1)
- IGC Proceedings (1997-2023) (1)
- Journal of the Department of Agriculture, Western Australia, Series 4 (1)
- Journal of the Minnesota Academy of Science (1)
- Masters Theses 1911 - February 2014 (1)
- Research outputs 2014 to 2021 (1)
- The Cardinal Edge (1)
- USF Tampa Graduate Theses and Dissertations (1)
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Articles 1 - 19 of 19
Full-Text Articles in Life Sciences
Pollen-Mediated Gene Flow From Genetically Modified Herbicide Resistant Creeping Bentgrass, L. S. Watrud, E. H. Lee, A. Fairbrother, C. Burdick, J. R. Reichman, M. Bollman, M. Storm, G. King, P. K. Van De Water
Pollen-Mediated Gene Flow From Genetically Modified Herbicide Resistant Creeping Bentgrass, L. S. Watrud, E. H. Lee, A. Fairbrother, C. Burdick, J. R. Reichman, M. Bollman, M. Storm, G. King, P. K. Van De Water
IGC Proceedings (1997-2023)
Approximately 162 ha of multiple experimental fields of creeping bentgrass (Agrostis stolonifera L.) genetically modified for resistance to Roundup ®herbicide, were planted in central Oregon in 2002. When the fields flowered for the first time in the summer of 2003, a unique opportunity was presented to evaluate methods to monitor potential pollen-mediated gene flow from the experimental GM crop fields to compatible sentinel and resident plants that were located in surrounding, primarily non-agronomic areas.
The Effects Of Ppal-1 In Arabidopsis Gamete Development, Amanda J White, Susana Perez-Martinez, Mark P. Running
The Effects Of Ppal-1 In Arabidopsis Gamete Development, Amanda J White, Susana Perez-Martinez, Mark P. Running
The Cardinal Edge
Prenylation is a type of post-translational modification in which a 15- or 20-carbon lipid is added to the carboxyl (C) terminus of the protein. Arabidopsis thaliana contains the PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT-LIKE (PPAL) gene, which encodes a protein with homology to the α-subunits of the three known prenylation enzymes, PFT, PGGT, and Rab-GGT. We previously identified two mutations in PPAL, one of which is ppal-1, which contains a T-DNA insertion in the fourth intron. We have previously observed that self-fertilizing heterozygous ppal-1 plants produce progeny in which homozygous ppal-1 is underrepresented. This project attempts to ascertain …
Reproductive Ecology And Phenology Of Thalassia Testudinum (Hydrocharitaceae) In Tampa Bay, Florida, Sheila Scolaro
Reproductive Ecology And Phenology Of Thalassia Testudinum (Hydrocharitaceae) In Tampa Bay, Florida, Sheila Scolaro
USF Tampa Graduate Theses and Dissertations
Successful sexual reproduction and recolonization are critical in maintaining genetic diversity within seagrass meadows. Thalassia testudinum flower, fruit and seedling production were assessed visually at 10 sites within Tampa Bay, Florida approximately every 4 weeks from May to July 2017 to determine if there is spatial and temporal variation in sexual reproductive effort and if location within a meadow effects sexual reproductive effort. Results from this study reveal strong temporal variability throughout the reproductive season. The month of May was observed as peak Thalassia testudinum anthesis and June was peak fruiting in Tampa Bay during 2017. Flower and fruit density …
Flower Visitation In Relation To Pollen And Nectar Nutrition: Implications For Pollinator Habitat And Conservation, Megan E. Leach
Flower Visitation In Relation To Pollen And Nectar Nutrition: Implications For Pollinator Habitat And Conservation, Megan E. Leach
Electronic Theses and Dissertations
Pollination of both wild and crop plants is at a crossroads; honey bee populations are experiencing losses at a higher rate than ever before, and some native bee species are declining in abundance to the point of being listed as endangered species. A few examples of these threats include pesticide exposure, habitat loss, and climate change. In response to bee population declines, conservation efforts have been initiated to increase habitat quality for bees by planting pollinator reservoirs or gardens. Plants provide nutrition to bees in the form of pollen and nectar. Several studies have shown links between higher nutritional quality …
Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff
Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff
Department of Agronomy and Horticulture: Faculty Publications
We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.
The Effect Of Insects On Seed Set Of Ozark Chinquapin, Castanea Ozarkensis, Colton Zirkle
The Effect Of Insects On Seed Set Of Ozark Chinquapin, Castanea Ozarkensis, Colton Zirkle
Graduate Theses and Dissertations
Ozark chinquapin (Castanea ozarkensis), once found throughout the Interior Highlands of the United States, has been decimated across much of its range due to accidental introduction of chestnut blight, Cryphonectria parasitica. Efforts have been made to conserve and restore C. ozarkensis, but success requires thorough knowledge of the reproductive biology of the species. Other Castanea species are reported to have characteristics of both wind and insect pollination, but pollination strategies of Ozark chinquapin are unknown. Experiments were conducted to determine the influence of insects on successful pollination of C. ozarkensis, as measured by production of burs and seeds. Exclosure treatments …
Muts Homolog1 Silencing Mediates Orf220 Substoichiometric Shifting And Causes Male Sterility In Brassica Juncea, Na Zhao, Xinyue Xu, Yashitola Wamboldt, Sally Ann Mackenzie, Xiaodong Yang, Zhongyuan Hu, Jinghua Yang, Mingfang Zhang
Muts Homolog1 Silencing Mediates Orf220 Substoichiometric Shifting And Causes Male Sterility In Brassica Juncea, Na Zhao, Xinyue Xu, Yashitola Wamboldt, Sally Ann Mackenzie, Xiaodong Yang, Zhongyuan Hu, Jinghua Yang, Mingfang Zhang
Department of Agronomy and Horticulture: Faculty Publications
Cytoplasmic male sterility (CMS) has consistently been associated with the expression of mitochondrial open reading frames (ORFs) that arise from genomic rearrangements. Spontaneous fertility reversion in CMS has been observed in several cases, but a clear understanding of fertility reversion controlled by nuclear genetic influences has been lacking. Here, we identified spontaneous fertile revertant lines for Brassica juncea CMS cytoplasm in which the mitochondrial genome has undergone substoichiometric shifting (SSS) to suppress ORF220 copy number. We placed ORF220, with or without a mitochondrial targeting presequence, under the control of the CaMV35S and AP3 promoters in Arabidopsis to confirm that ORF220 …
Mechanosensitive Channel Msl8 Regulates Osmotic Forces During Pollen Hydration And Germination, Eric S. Hamilton, Gregory S. Jensen, Grigory Maksaev, Andrew Katims, Ashley M. Sherp, Elizabeth S. Haswell
Mechanosensitive Channel Msl8 Regulates Osmotic Forces During Pollen Hydration And Germination, Eric S. Hamilton, Gregory S. Jensen, Grigory Maksaev, Andrew Katims, Ashley M. Sherp, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
Pollen grains undergo dramatic changes in cellular water potential as they deliver the male germ line to female gametes, and it has been proposed that mechanosensitive ion channels may sense the resulting mechanical stress. Here, we identify and characterize MscS-like 8 (MSL8), a pollen-specific, membrane tension–gated ion channel required for pollen to survive the hypoosmotic shock of rehydration and for full male fertility. MSL8 negatively regulates pollen germination but is required for cellular integrity during germination and tube growth. MSL8 thus senses and responds to changes in membrane tension associated with pollen hydration and germination. These data further suggest that …
Pore Selectivity And Gating Of Arabidopsis Nodulin 26 Intrinsic Proteins And Roles In Boric Acid Transport In Reproductive Growth, Tian Li
Doctoral Dissertations
Plant nodulin-26 intrinsic proteins (NIPs) are members of the aquaporin superfamily that serve as multifunctional channels of uncharged metabolites and water. They share the same canonical hourglass fold as the aquaporin family. The aromatic arginine (ar/R) selectivity filter controls transport selectivity based on size, hydrophobicity, and hydrogen bonding with substrates. In Arabidopsis thaliana, NIP II subclass proteins contain a conserved ar/R “pore signature” that is composed of Alanine at the helix 2 position (H2), Valine/Isoleucine at the helix 5 position (H5), and an Alanine (LE1) and an invariant Arginine (LE2) at the two loop E positions. In this study, …
The Movement Ecology Of Seagrasses, Kathryn Mcmahon, Kor-Jent Van Dijk, Leonardo Ruiz-Montoya, Gary A. Kendrick, Siegfried L. Krauss, Michelle Waycott, Jennifer Verduin, Ryan Lowe, John Statton, Eloise Brown, Carlos Duarte
The Movement Ecology Of Seagrasses, Kathryn Mcmahon, Kor-Jent Van Dijk, Leonardo Ruiz-Montoya, Gary A. Kendrick, Siegfried L. Krauss, Michelle Waycott, Jennifer Verduin, Ryan Lowe, John Statton, Eloise Brown, Carlos Duarte
Research outputs 2014 to 2021
A movement ecology framework is applied to enhance our understanding of the causes, mechanisms and consequences of movement in seagrasses: marine, clonal, flowering plants. Four life-history stages of seagrasses can move: pollen, sexual propagules, vegetative fragments and the spread of individuals through clonal growth. Movement occurs on the water surface, in the water column, on or in the sediment, via animal vectors and through spreading clones. A capacity for long-distance dispersal and demographic connectivity over multiple timeframes is the novel feature of the movement ecology of seagrasses with significant evolutionary and ecological consequences. The space–time movement footprint of different life-history …
A Comparative Analysis Of The Relative Water Content Of The Pollen Of Early Diverging Angiosperms, Andrew Robert Moffatt
A Comparative Analysis Of The Relative Water Content Of The Pollen Of Early Diverging Angiosperms, Andrew Robert Moffatt
Chancellor’s Honors Program Projects
No abstract provided.
Developing Biocontainment Strategies To Suppress Transgene Escape Via Pollen Dispersal From Transgenic Plants, Hong Seok Moon
Developing Biocontainment Strategies To Suppress Transgene Escape Via Pollen Dispersal From Transgenic Plants, Hong Seok Moon
Doctoral Dissertations
Genetic engineering is important to enhance crop characteristics and certain traits. Genetically engineered crop cultivation brings environmental and ecological concerns with the potential of unwanted transgene escape and introgression. Transgene escape has been considered as a major environmental and regulatory concern. This concern could be alleviated by appropriate biocontainment strategies. Therefore, it is important to develop efficient and reliable biocontainment strategies.
Removing transgenes from pollen has been known to be the most environmentally friendly biocontainment strategy. A transgene excision vector containing a codon optimized serine resolvase CinH recombinase (CinH) and its recognition sites RS2 were constructed and transformed into tobacco …
Developmental Evolution Of The Progamic Phase In Nymphaeales, Mackenzie Lorraine Taylor
Developmental Evolution Of The Progamic Phase In Nymphaeales, Mackenzie Lorraine Taylor
Doctoral Dissertations
The period between pollination and fertilization, or the progamic phase, is a critical life history stage in seed plants and innovations in this life history stage are hypothesized to have played an important role in the diversification of flowering plants. Over the course of this dissertation research, I investigated programic phase development in Nymphaeales (water lilies), an ancient angiosperm lineage that diverged from the basalmost or next most basal node of the angiosperm phylogenetic tree and that is represented in the oldest angiosperm fossil record. I used field experiments and microscopy to document pollination biology, breeding system, and reproductive developmental …
Functional Analysis Of Receptor-Like Kinases In Pollen-Pistil Interactions In Arabidopsis Thaliana, Mini Aggarwal
Functional Analysis Of Receptor-Like Kinases In Pollen-Pistil Interactions In Arabidopsis Thaliana, Mini Aggarwal
Masters Theses 1911 - February 2014
Rac/Rop GTPases are molecular switches in plants that control the growth of polarized cells such as pollen tubes and root hairs, differentiation, development, actin dynamics, production of reactive oxygen species (ROS) and disease resistance. These small GTPases are activated by guanine nucleotide exchange factors (GEFs) that replace GDP for GTP and are referred to as RopGEFs in plants. To identify upstream components of the RopGEF regulated signaling pathways, GEF1 from Arabidopsis thaliana was used as a bait to screen a seedling cDNA library in a yeast two-hybrid system. This yielded members of a small family of the Catharanthus roseus Receptor-like …
Nectar Secondary Compounds Affect Self-Pollen Transfer: Implications For Female And Male Reproduction, Rebecca E. Irwin, Lynn S. Adler
Nectar Secondary Compounds Affect Self-Pollen Transfer: Implications For Female And Male Reproduction, Rebecca E. Irwin, Lynn S. Adler
Dartmouth Scholarship
Pollen movement within and among plants affects inbreeding, plant fitness, and the spatial scale of genetic differentiation. Although a number of studies have assessed how plant and floral traits influence pollen movement via changes in pollinator behavior, few have explored how nectar chemical composition affects pollen transfer. As many as 55% of plants produce secondary compounds in their nectar, which is surprising given that nectar is typically thought to attract pollinators. We tested the hypothesis that nectar with secondary compounds may benefit plants by encouraging pollinators to leave plants after visiting only a few flowers, thus reducing self-pollen transfer. We …
Pollen Of The Myricaceae - A Preliminary Report, Marshall D. Sundberg
Pollen Of The Myricaceae - A Preliminary Report, Marshall D. Sundberg
Journal of the Minnesota Academy of Science
Pollen of 16 species and one variety of the Myricaceae was examined, including members of each of the genera proposed by Chevalier (1901) in addition to Canacomyrica (Guillaumin, 1939). Comparison of pollen characters within genera, within sections, between sections, and between genera suggests that the Myricaceae can be divided into four genera: Canacomyrica; Comptonia; Gale; and Myrica. The genus Myrica can be further divided into three sections; Cerophora, Fava, and Morella.
Plants And Asthma, Robert Dunlop Royce
Plants And Asthma, Robert Dunlop Royce
Journal of the Department of Agriculture, Western Australia, Series 4
ASTHMA is defined in the dictionary as being a disease characterised by recurring attacks of difficulty in breathing, and the causes are stated to include the inhalation of dust, or the odour of plants, flowers or animals.
Hay fever is a similar condition and this too, is caused by plants, usually at the time of flowering, when large quantities of pollen are produced.
The Significance Of Xenia Effects On The Kernel Weight Of Corn, T. A. Kiesselbach
The Significance Of Xenia Effects On The Kernel Weight Of Corn, T. A. Kiesselbach
Historical Research Bulletins of the Nebraska Agricultural Experiment Station
The purpose of this paper is to appraise the xenia effects of the pollen parent on the kernel weight of corn (Zea mays L.), and to interpret so far as possible their genetic, physiologic and applied significance. The conclusions will be based on local experiments and a review of the literature.
The Effects Of Thermal-Neutron Irradiation Of Maize And Barley Kernels, John W. Schmidt, E. F. Frolik
The Effects Of Thermal-Neutron Irradiation Of Maize And Barley Kernels, John W. Schmidt, E. F. Frolik
Historical Research Bulletins of the Nebraska Agricultural Experiment Station
It is the purpose of this paper to report the effects of thermal-neutron irradiation of maize and barley kernels on subsequent germination, plant establishment, and early plant development. Comparisons are made with equivalent neutron treatments of maize pollen, results of which have been reported in part in previous publications.