Life Sciences Commons^™

Where Are All Of Arkansas' Chinquapins? An Ecological Assessment Of Castanea Throughout The State, Logan Pierce Estes

Graduate Theses and Dissertations

Around the turn of the twentieth-century, the chestnut blight fungus (Cryphonectria parasitica) was accidentally introduced into North America. This strong pathogen, which specializes on trees of the genus Castanea, spread rapidly and within half a century had nearly extirpated North America’s Castanea natives from their ranges. During this catastrophe, the American chestnut (Castanea dentata) garnered much of the scientific attention, pushing the other Castanea natives – the chinquapins – to the wayside. More than a century following the spread of the blight, little research into the ecology of North America’s chinquapins had been performed, leaving these trees significantly underrepresented. The …

Evaluation Of Rice Stink Bug, Oebalus Pugnax (F.), Damage And Monitoring Techniques In Rice, Oryza Sativa L., And Grain Sorghum, Sorghum Bicolor (L.), Aaron Joseph Cato

Graduate Theses and Dissertations

Rice stink bug, Oebalus pugnax (F.), is a serious pest of headed rice, Oryza sativa L. and an occasional pest of heading grain sorghum in the Mid-south. Work from this dissertation focuses on resolving gaps in and knowledge of rice stink bug sampling and management, and attempts to create a basis for rice stink bug damage assessment in future studies.

Field experiments were conducted from 2016-2018 to asses variation in sweep net sampling by observing producers, researchers, extension personnel, consultants and their workers. Large levels of variation were found in sweep lengths between observed sweepers and reliability of smaller sweep …

Physiological Characterization Of The Soynam Parental Lines Under Field Conditions, Akshita Mishra

Graduate Theses and Dissertations

The narrow genetic pool of soybean (Glycine max L. Merr.) in North America can limit its future yield gains. Among the worldwide germplasm collection of 45,000 unique landraces, only 80 contribute 99% to the collective parentage of North American soybean cultivars. Among these 80 landraces, just 17 contribute to 86% of the collective parentage of the modern cultivars. The Soybean Nested Association Mapping population (SoyNAM) was therefore developed with the objective of diversifying the soybean gene pool. Forty diverse soybean genotypes from maturity groups (MG) 1 through 5 were crossed with a common MG 3 parent to develop 40 recombinant …

Influence Of Loss Of Function Of The Fatty Acid Desaturase 7 Gene On Photosynthetic Activity And Foliar Redox Status, Janithri Savindya Wickramanayake

Graduate Theses and Dissertations

Fatty Acid Desaturase7 (FAD7) is a chloroplast-localized enzyme that converts 16 and 18 carbon dienoic fatty acids to trienoic fatty acids. The suppressor of prosystemin-mediated response2 (spr2) mutant in tomato (Solanum lycopersicum) and the fad7-1 mutant in Arabidopsis (Arabidopsis thaliana) result in the loss of function of FAD7, which alter the fatty acid profiles of chloroplast membranes and enhance resistance against aphids. This research contributes toward the long-term goal of identifying factors that determine aphid resistance in FAD7 mutants. Previous data suggested constitutive differences between spr2 and WT including increased expression of genes associated with photosynthesis and differences in redox …

Effect Of Allelic Variation In Rht Loci On Plant Height And Grain Yield Of Soft Red Winter Wheat (Triticum Aestivum L.), Habib Hayat

Graduate Theses and Dissertations

Plant height in wheat (Triticum aestivum L.) is controlled in large part by two major Rht genes, Rht-B1 and Rht-D1, which pleiotropically impact lodging and grain yield. Prior to the Green Revolution, wheat varieties contained only ‘wild-type’ Rht alleles (Rht-B1a and Rht-D1a) and were tall and prone to lodging. Introgression of a semi-dominant mutation at either of these two loci (Rht-B1b or Rht-D1b) results in a semi-dwarf phenotype and reduced plant height. When combined (Rht-B1b and Rht-D1b) an extremely short double-dwarf phenotype is observed. The objective of this study was to determine the impact of allelic variation in Rht-B1 and …

Dissection Of Soil Waterlogging Tolerance In Soft Red Winter Wheat Using Genomic Approaches, Marlovi Andrea Acuna-Galindo

Graduate Theses and Dissertations

Genomic methods including genome wide association analysis (GWAS), genomic selection (GS) and RNA-seq allow for faster selection of superior breeding lines and for identification and resolution of candidate genes. A panel of 240 soft red winter wheat (Triticum aestivum L.) cultivars and breeding lines were subjected to soil waterlogging stress over two seasons at Stuttgart, AR and St. Joseph, LA, US. Total concentrations of P, K, Ca, Mg, Mn, Fe, Al, B, Cu, Na, S and Zn were determined in wheat shoots post-waterlogging using inductively coupled plasma spectroscopy. Yield components kernel number per spike (KNPS), kernel weight per spike (KWS) …

Association Mapping And Genomic Selection For Yield And Agronomic Traits In Soft Winter Wheat, Dennis Bulusan Lozada

Graduate Theses and Dissertations

Tools such as genome-wide association study (GWAS) and genomic selection (GS) have expedited the development of crops with improved genetic potential. While GWAS aims to identify significant markers associated with a trait of interest, the goal of GS is to utilize all marker effects to predict the performance of new breeding lines prior to testing. A GWAS for grain yield (GY), yield components, and agronomic traits was conducted using a diverse panel of 239 soft winter wheat (SWW) lines evaluated in eight site-years in Arkansas and Oklahoma. Broad sense heritability of GY (H2=0.48) was moderate compared to other traits including …

Identification And Characterization Of Salinity Tolerance Genes By Activation Tagging In Arabidopsis, Jawaher Alkahtani

Graduate Theses and Dissertations

Salinity often affects irrigated areas in arid and semi-arid regions of the world. The existence and accumulation of soluble salts in the soil layers limit the growth of crops essential for our food. Salt stress dramatically affects plant growth, plant development, as well as crop yield. Arabidopsis thaliana is the plant model that provides a comprehensive knowledge of plant development, genetics and physiology, and response to abiotic stresses such as salinity. The redundancy of genes due to duplication, even in the simple model genome of Arabidopsis, limits the value of knockout (KO) mutagenesis to provide complete information on gene function. …

Combining Translational And Functional Genomic Approaches To Augment Management Strategies Of Plant Parasitic Nematodes, Sandeep Sharma Khatiwada

Graduate Theses and Dissertations

Plant-parasitic nematodes pose a major threat to crop yield worldwide. Discontinued use of harmful chemicals has prompted the search for alternative management strategies that are effective yet environmentally friendly. Harpin proteins, which are derived from bacteria, and nematophagous fungi, natural predators of nematodes, are ideal for biological control of plant-parasitic nematodes. However, research on the efficacy of harpin proteins on nematodes, and biology of nematophagous fungi is minimal. Previously, a taxonomically uncharacterized nematophagous fungus designated ARF18 effectively suppressed nematode populations in soil. The overarching goal of this dissertation is to utilize applied and functional genomic approaches to augment management of …

Non-Target-Site Based Tolerance To Herbicides In Amaranthus Palmeri, Reiofeli Algodon Salas-Perez

Graduate Theses and Dissertations

Palmer amaranth, one of the most aggressive and damaging broadleaf weeds in the USA, has evolved resistance to multiple herbicide modes of action. The overall objective of this research was to elucidate the mechanisms by which Palmer amaranth adapt to herbicide selection stress. This research aimed (1) to evaluate the efficacy of fomesafen, glufosinate, glyphosate and trifloxysulfuron to Amaranthus populations; (2) identify candidate genes for endowing tolerance to glufosinate; (3) investigate the involvement of non-target-site resistance (NTSR) mechanism in an ALS-resistant population; and (4) to examine the molecular basis of resistance to PPO inhibitors in Palmer amaranth populations from Arkansas. …