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Searching For Potential Binding Partners Of Arabidopsis Β-Amylase2 Using Yeast 2-Hybridization, Megan E. Hines
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase2 Using Yeast 2-Hybridization, Megan E. Hines
Senior Honors Projects, 2010-2019
BAM2 is a chloroplast-targeted member of the β-amylase gene family that currently has an unknown function in starch hydrolysis. Previous research indicated that BAM2 did not have significant catalytic activity, but, because the gene is highly conserved and there is a starch-excess phenotype in older plants lacking BAM2, it was hypothesized that BAM2 may instead have a regulatory function. Many regulatory proteins function by interacting with other proteins, so we wanted to test for potential protein binding partners for BAM2 using the yeast two-hybrid system. A plasmid containing the BAM2 gene from Arabidopsis thaliana was co-transformed into Y190-strain yeast ( …