Life Sciences Commons^™

Suberin Biosynthesis And Deposition In The Wound-Healing Potato (Solanum Tuberosum L.) Tuber Model, Kathlyn Natalie Woolfson

Electronic Thesis and Dissertation Repository

Suberin is a heteropolymer comprising a cell wall-bound poly(phenolic) domain (SPPD) covalently linked to a poly(aliphatic) domain (SPAD) that is deposited between the cell wall and plasma membrane. Potato tuber skin contains suberin to protect against water loss and microbial infection. Wounding triggers suberin biosynthesis in usually non-suberized tuber parenchyma, providing a model system to study suberin production. Spatial and temporal coordination of SPPD and SPAD-related metabolism are required for suberization, as the former is produced soon after wounding, and the latter is synthesized later into wound-healing. Many steps involved in suberin biosynthesis remain uncharacterized, and the mechanism(s) that regulate …

Evaluation Of The Water Stress-Inducible Promoter Wsi18 In The Model Monocot Brachypodium Distachyon, Patrick D. Langille

Electronic Thesis and Dissertation Repository

Water deficit-inducible promoters that function in multiple species are valuable components for engineering stress-tolerant crops. Wsi18 is a water deficit-inducible promoter native to Oryza sativa. In this study, Brachypodium distachyon (B. distachyon) was used to determine if Wsi18 retained its water deficit-inducible characteristics in another monocot. Transgenic B. distachyon plants, in which the Wsi18 promoter drove the expression of the uidA reporter gene, were developed and exposed to osmotic stress generated by mannitol, salt stress conditions, and the water deficit-signaling phytohormone abscisic acid (ABA). GUS histochemical assays demonstrated increased uidA expression in the leaves and stem of …

Study Of Thermotolerance Mechanism In Gossypium Hirsutum Through Identification Of Heat Stress Genes, Jin Zhang

Graduate Theses and Dissertations

ABSTRACT

Heat stress causes major losses to cotton seed and lint yield. Introduction of heat stress tolerance to Arkansas cotton varieties is highly desirable. However, very little is known about the molecular basis of heat stress tolerance in cotton. The present study attempted to identify heat stress tolerance genes in two heat-tolerant cotton cultivars, VH260 and MNH456, originating from Pakistan. Towards this, the expression profile of the cotton orthologs of sevenArabidopsisheat stress tolerance genes was studied in these two cultivars, and compared with the two heat-susceptible cotton cultivars, ST213 and ST4288, originating from Mississippi Delta region. In addition, physiological parameters …