Life Sciences Commons^™

Is Protection Against Florivory Consistent With The Optimal Defense Hypothesis?, Adrienne L. Godschalx, Lauren Stady, Benjamin Watzig, Daniel J. Ballhorn

Biology Faculty Publications and Presentations

Background: Plant defense traits require resources and energy that plants may otherwise use for growth and reproduction. In order to most efficiently protect plant tissues from herbivory, one widely accepted assumption of the optimal defense hypothesis states that plants protect tissues most relevant to fitness. Reproductive organs directly determining plant fitness, including flowers and immature fruit, as well as young, productive leaf tissue thus should be particularly well-defended. To test this hypothesis, we quantified the cyanogenic potential (HCNp)—a direct, chemical defense—systemically expressed in vegetative and reproductive organs in lima bean (Phaseolus lunatus), and we tested susceptibility of these …

Small Unmanned Aerial Vehicles (Micro-Uavs, Drones) In Plant Ecology, Mitchell B. Cruzan, Ben G. Weinstein, Monica R. Grasty, Brendan F. Kohrn, Elizabeth C. Hendrickson, Tina M. Arredondo, Pamela G. Thompson

Biology Faculty Publications and Presentations

Premise of the study: Low-elevation surveys with small aerial drones (micro–unmanned aerial vehicles [UAVs]) may be used for a wide variety of applications in plant ecology, including mapping vegetation over small- to medium-sized regions. We provide an overview of methods and procedures for conducting surveys and illustrate some of these applications.

Methods: Aerial images were obtained by flying a small drone along transects over the area of interest. Images were used to create a composite image (orthomosaic) and a digital surface model (DSM). Vegetation classification was conducted manually and using an automated routine. Coverage of an individual species …

Distance And Sex Determine Host Plant Choice By Herbivorous Beetles, Daniel J. Ballhorn, Stefanie Kautz, Martin Heil

Biology Faculty Publications and Presentations

Background: Plants respond to herbivore damage with the release of volatile organic compounds (VOCs). This indirect defense can cause ecological costs when herbivores themselves use VOCs as cues to localize suitable host plants. Can VOCs reliably indicate food plant quality to herbivores?

Methodology: We determined the choice behavior of herbivorous beetles (Chrysomelidae: Gynandrobrotica guerreroensis and Cerotoma ruficornis) when facing lima bean plants (Fabaceae: Phaseolus lunatus) with different cyanogenic potential, which is an important constitutive direct defense. Expression of inducible indirect defenses was experimentally manipulated by jasmonic acid treatment at different concentrations. The long-distance responses of male and female beetles to …

Evidence Of Reduced Arbuscular Mycorrhizal Fungal Colonization In Multiple Lines Of Bt Maize, Tanya E. Cheeke, Todd N. Rosenstiel, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Premise of the Study: Insect-resistant Bacillus thuringiensis (Bt) maize is widely cultivated, yet few studies have examined the interaction of symbiotic arbuscular mycorrhizal fungi (AMF) with different lines of Bt maize. As obligate symbionts, AMF may be sensitive to genetic changes within a plant host. Previous evaluations of the impact of Bt crops on AMF have been inconsistent, and because most studies were conducted under disparate experimental conditions, the results are difficult to compare. Methods: We evaluate AMF colonization in nine Bt maize lines, differing in number and type of engineered trait, and five corresponding near-isogenic parental (P) base hybrids …

Sex-Specific Variation In The Interaction Between Distichlis Spicata (Poaceae) And Mycorrhizal Fungi, Sarah M. Eppley, Charlene Ashley Mercer, Christian Haaning, Camille Brianne Graves

Biology Faculty Publications and Presentations

Associations between mycorrhizal fungi and plants can influence intraspecific competition and shape plant population structure. While variation in plant genotypes is known to affect mycorrhizal colonization in crop systems, little is known about how genotypes affect colonization in natural plant populations or how plant sex might influence colonization with mycorrhizal fungi in plant species with dimorphic sexual systems. In this study, we analyzed mycorrhizal colonization in males and females of the wetland dioecious grass Distichlis spicata, which has spatially segregated sexes. Our results suggest that D. spicata males and females interact with mycorrhizal fungi differently. We discuss the implications for …

Investigating The Relationship Between Cryptococcus Fagisuga And Fagus Grandifolia In Great Smoky Mountains National Park, Ashley B. Morris, Randall L. Small, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Focuses on a study which investigated the relationship between Cryptococcus fagisuga and Fagus grandifolia in Great Smoky Mountains National Park. Potential for genetic resistance; Methods; Results and discussion.

Intrapopulation Sex Ratio Variation In The Salt Grass Distichlis Spicata, Sarah M. Eppley, Maureen L. Stanton, Richard K. Grosberg

Biology Faculty Publications and Presentations

In many dioecious plant populations, males and females appear to be spatially segregated, a pattern that is difficult to explain given its potentially high costs. However, in asexually propagating species, spatial segregation of the sexes may be indistinguishable from superficially similar patterns generated by random establishment of a few genets followed by extensive clonal spread and by gender-specific differences in rates of clonal spread. In populations where a significant fraction of individuals are not flowering and gender cannot be assigned to this fraction, apparent spatial segregation of the sexes may be due to differential flowering between the sexes. We confirm …

Genetic Markers In Plant Evolutionary Ecology, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Genetic markers have provided plant ecologists with a method of assessing levels of genetic relatedness among individuals and populations. In recent years a number of techniques based on DNA sequence variation have been developed to complement allozyme methods that are already widely used. Some of these new markers are more variable than protein-based markers, allowing more precise estimates of genetic differences among individuals and populations. Other DNA-based markers are based on organelle genomes that are inherited uniparentally. These cytoplasmic markers can provide a method for assessing the separate effects of seed and pollen dispersal on gene flow within and among …

Temporal Patterns Of Nectar And Pollen Production In Aralia Hispida: Implications For Reproductive Success, James D. Thomas, Mary A. Mckenna, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Large plants of Aralia hispida present their pollen and nectar in hundreds of small flowers than open sequentially over 2—3 wk in a pattern of synchronized protandry that alternates male and female phases. The primary pollinators, bumble bees, are able to discover individual plants with elevated levels of either nectar or pollen, and to return to them more often than to less rewarding plants. Both pollen and nectar are presented gradually over time in such a way as to favor traplining behavior by the bees, with many, frequent visits. In controlled environments, lifetime sugar production per flower varies among plants, …