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Plant Biology

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University of Tennessee, Knoxville

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Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Constitutive Expression Of Thioglucoside Glucohydrolase 1 (Tgg1) Decreases Intercellular Trafficking In Arabidopsis Thaliana, Alessandro Francesco Sarno Apr 2018

Constitutive Expression Of Thioglucoside Glucohydrolase 1 (Tgg1) Decreases Intercellular Trafficking In Arabidopsis Thaliana, Alessandro Francesco Sarno

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Plasmodesmata (PD) are pores that traverse plant cell walls, providing a route for intercellular trafficking of essential metabolites, nutrients, and signaling molecules between adjacent plant cells, thereby aiding communication. The increased size exclusion limit 2 (ise2) mutant of Arabidopsis thaliana has an increased abundance of branched PD, as well as a greater flux of intercellular trafficking. A search for proteins that interact with ISE2 identified THIOGLUCOSIDE GLUCOHYDROLASE 2 (a myrosinase). A. thaliana also encodes a second, closely-related myrosinase, TGG1. Myrosinases are enzymes that catalyze the hydrolysis of glucosinolates, a type of secondary metabolite that are amino acid derivatives. The breakdown …


Production, Purification, And X-Ray Crystallography Of The Potra Domains Of Pstoc75, Gabriela Jordan Little Apr 2015

Production, Purification, And X-Ray Crystallography Of The Potra Domains Of Pstoc75, Gabriela Jordan Little

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Plastids arose via endosymbiosis when a cyanobacterium was engulfed by a primitive eukaryote. The cyanobacterium was enslaved by the eukaryote, eventually giving rise to a new organelle, termed the plastid. The transition from a free-living cyanobacterium to a cell-dependent organelle demonstrates how the chloroplast ancestor underwent many changes in its physiology and biological processes. The majority of the DNA belonging to the cyanobacterium was scavenged by the nucleus of the host cell. This led to higher fidelity of genetic duplication, due to the proofreading abilities of the DNA polymerase of the host cell. This left the cell with the problem …


Phosphorylation Regulates Myosin Driven Organelle Movements, Peter Andrew Duden Mar 2013

Phosphorylation Regulates Myosin Driven Organelle Movements, Peter Andrew Duden

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Cytoplasmic streaming in plant cells is the continuous flow of cytoplasm and organelles throughout the cell, with the first observation of cytoplasmic streaming being publicized in 1774. However, the mechanism of cytoplasmic streaming remained unclear until components of the cytoskeleton were researched. Research now supports that the motive force generating cytoplasmic streaming is the interaction of myosin XI motor proteins with organelles while sliding along actin filaments. From this, a key topic of interest is how myosin driven organelle movement is regulated. Our research focuses on whether phosphorylation affects the regulation of myosin XI motor proteins. Specifically, the goal of …